The formation of mRNA 3′‐ends in plants

The Polyadenylation of RNA in Plants

“…A detailed picture of poly(A) signals has emerged from studies of a number of plant genes. A number of reviews published in the past 3 years provide a comprehensive treatment of this subject and may be consulted for specific information (Hunt, 1994;Wu et al, 1995;Rothnie, 1996).…”

“…NUEs are A-rich sequences, between 6 and 10 nts, that are situated between 10 and 40 nts from their associated polyadenylation site. Based on similarities in sequence composition and relative location, it has been suggested that NUEs are functionally analogous to the mammalian polyadenylation signal AAUAAA (Wu et al, 1995). However, the contrasting properties of NUEs and AAUAAA, as revealed by directed mutagenesis studies (Hunt, 1994;Rothnie, 1996) Wahle (1995).…”

Divergent Fructokinase Genes Are Differentially Expressed in Tomato

“…An AATAAA motif was located at 71 bp upstream from the polyadenylation site, and a GT-rich sequence was also located upstream from the motif in the 3' untranslated region. In plant mRNAs an AAUAAA-like sequence is typically located 10 to 30 nucleotides upstream from the polyadenylation site and a GU-rich sequence is typically located upstream from the polyadenylation signal (Wu et al, 1995). Because there were no other AATAAAlike sequences between the AATAAA motif and the polyadenylation site in the 3' untranslated region of Frk2 cDNA, it is appears that the Frk2 transcript has a polyadenylation signal unusually far from the polyadenylation site.…”

Alternative processing of the maize Ac transcript in Arabidopsis

“…The correctly polyadenylated transposase transcripts are virtually indistinguishable in Arabidopsis and, due the relatively small variance in transcript size and low transcript abundance, they have not been resolved clearly. upstream regulatory element in the CaMV polyadenylation signal (Rothnie et aL, 1994) and a series of TG-like repeats that may be analogous to a motif found in the maize zein mRNA polyadenylation signal (Wu et aL, 1993(Wu et aL, , 1995.…”

“…Using Northern blotting, we confirmed that the Ac message was correctly processed in tobacco, and provided evidence that the 3'-truncated form of transposase mRNA is by far the most prevalent form of transcript in Arabidopsis. Recent studies of transcript polyadenylation in plants have suggested that there is a conservation of the mRNA 3'-end processing machinery between monocot and dicot plant species (Wu et al, 1994(Wu et al, , 1995. The frequent employment by Arabidopsis of alternative poly(A) sites far from the correct region of transcript polyadenylation suggests that, as with transcript splicing, this species does not conform to existing models.…”