1997
DOI: 10.1046/j.1365-313x.1997.11050933.x
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Alternative processing of the maize Ac transcript in Arabidopsis

Abstract: SummaryThe successful application of the maize transposable element system AciDs as a genome mutagen in heterologous plant species has recently proved the versatility and power of this technique in plant molecular biology. However, the frequency of Ac/Ds transposition is considerably lower in Arabidopsis thaliana than in most other dicot plant species that have been studied. Since previous research has established that transcripts derived from monocot genes can be alternatively processed in dicot plants, we ha… Show more

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Cited by 22 publications
(28 citation statements)
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References 38 publications
(51 reference statements)
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“…Therefore, cop1-8 3 with the single splice site mutation displayed the same pattern of splicing (exon skipping) as cop1-8 in Arabidopsis. The differences between Arabidopsis and tobacco splicing emphasize the recent finding that different plant species may show variation in their ability to recognize and splice out different introns (Jarvis et al, 1997;Martin et al, 1997).…”
Section: Splicing Analysis Of Cop1-8mentioning
confidence: 81%
“…Therefore, cop1-8 3 with the single splice site mutation displayed the same pattern of splicing (exon skipping) as cop1-8 in Arabidopsis. The differences between Arabidopsis and tobacco splicing emphasize the recent finding that different plant species may show variation in their ability to recognize and splice out different introns (Jarvis et al, 1997;Martin et al, 1997).…”
Section: Splicing Analysis Of Cop1-8mentioning
confidence: 81%
“…This contention is supported by the finding that even a transcript normally produced in one plant species can be differentially polyadenylated when it is transcribed in another. Specifically, the maize activator (Ac) transposase transcript is polyadenylated at four sites within a 200-bp region of exon 2 when it is expressed in Arabidopsis plants (Jarvis et al, 1997;Martin et al, 1997). Recognition of these poly(A ϩ ) addition sites has been suggested to contribute to the low abundance of correctly processed transposase transcripts and hence the low frequency of transposition in this plant species.…”
Section: Discussionmentioning
confidence: 99%
“…5). Primers A-F and A-R are identical to primers ZZZ;aZZZ; by Martin et al (1997). Analysis of introns III and IV was performed using primers INT3-F (5′-GTCTGCGTTCAG-TGCTGGT-3′) and INT4-R (5′-CACTTGCTCACATCTGGAT-CA-3′) (Jarvis et al 1997) (see Fig.…”
Section: Rt-pcr Analysis Of Ac St Transcriptsmentioning
confidence: 99%
“…5C). While the sizes of these products do not correspond to incomplete processing of introns III and IV, they may represent products arising from the use of alternative 5′ and 3′ splice sites within intron IV (Martin et al 1997). Alternatively, they may represent non-specific products associated with the Ac st sequences present in all groups of plants.…”
Section: The Ac St Transcript Is Not Fully Processed In B Napusmentioning
confidence: 99%
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