The formation of an antibacterial agent–apatite composite coating on a polymer surface using a metastable calcium phosphate solution

Oyane, Ayako; Yokoyama, Yoshiro; Uchida, Masaki; Ito, Atsuo

doi:10.1016/j.biomaterials.2006.01.029

Cited by 57 publications

(59 citation statements)

References 28 publications

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“…19 During the subsequent immersion in the CP solution, ACP induces heterogeneous nucleation and crystal growth of HA at the specimen surface. The great advantage of our coating process is the immobilization of biomolecules (such as proteins, [20][21][22][23] antibiotics, 5 and DNA [24][25][26] ), which retain their biological activity, into the HA layer on polymer specimens. In the present study, laminin, [27][28][29] which is a major cell adhesion protein, was immobilized into the HA layer to increase the shear strength of the layer 30 and promote cell adhesion onto the layer, 20,31 which would facilitate implant-skin integration.…”

Section: Contract Grant Sponsors: Industrial Technology Research Granmentioning

confidence: 99%

“…Incorporation of antibiotics onto percutaneous devices and the usage of percutaneous devices in combination with dressings incorporating antibiotics have also been proposed. [2][3][4][5] However, the continuous use of antibiotics has a secondary risk of developing antibiotic-resistant bacteria. 6 Integration of a percutaneous device with the surrounding skin tissue would fill the gap between the device and the skin tissue and is likely to be a promising approach to preventing bacterial infection with no such secondary risk.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

et al. 2011

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A polymeric material coated with a hydroxyapatite (HA) layer would be useful as a flexible percutaneous device with good biocompatibility and resistance to bacterial infection. We have recently developed a simple, safe, and mild coating process to form an HA layer on the surfaces of polymeric materials. In this study, our coating process was applied to an ethylene-vinyl alcohol copolymer film. The resulting HA-coated film was percutaneously implanted in the scalp of a rat to examine the stability and biocompatibility of the HA layer. From the results of histological analysis, the HA layer remained undissolved on the film surface under the skin tissue even 3 days after implantation. Owing to the good biocompatibility of HA, the HA-coated film suppressed a host's foreign-body response and integrated with the surrounding skin tissue for as long as 14 days, in a similar fashion to a conventional percutaneous device composed of ceramic HA. Immobilization of a cell adhesion protein, laminin, into the HA layer was found to improve the adhesion strength between the film and the surrounding skin tissue without compromising good biocompatibility of HA. Our coating process to form HA and laminin-HA composite layers would be useful in fabricating polymeric percutaneous devices with a reduced risk of bacterial infection, although further in vivo studies are required.

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Section: Contract Grant Sponsors: Industrial Technology Research Granmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

et al. 2011

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“…The DA-Ap layers are thought to form in the coating solutions via the growth of an apatite layer on the EVOH surface and the simultaneous immobilization of DNA and antibody, on the basis of our previous report on the ACP-assisted biomimetic coating process [35]. During this coating process, a crucial step affecting the DNA and antibody contents of the DA-Ap layer could be the adsorption of DNA and antibody molecules through electrostatic interactions on apatite crystals or their precursors including an amorphous phase [36][37][38][39] coating solution resulted in the higher mean antibody content of the resulting DA-Ap layer ( figure 3(b)), probably by accelerating the antibody adsorption rate. Sample DA20 had a lower DNA content than samples DA5 and DA10 ( figure 3(a)), although the initial DNA concentration in the coating solutions was the same.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Yazaki

Oyane

Araki

et al. 2012

Science and Technology of Advanced Materials

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Surface-mediated gene transfer systems using apatite (Ap)-based composite layers have received increased attention in tissue engineering applications owing to their safety, biocompatibility and relatively high efficiency. In this study, DNA-antibody-apatite composite layers (DA-Ap layers), in which DNA and antibody molecules are immobilized within a matrix of apatite nanocrystals, were fabricated using a biomimetic coating process. They were then assayed for their gene transfer capability for application in a specific cell-targeted gene transfer. A DA-Ap layer that was fabricated with an anti-CD49f antibody showed a higher gene transfer capability to the CD49f-positive CHO-K1 cells than a DNA-apatite composite layer (D-Ap layer). The antibody facilitated the gene transfer capability of the DA-Ap layer only to the specific cells that were expressing corresponding antigens. When the DA-Ap layer was fabricated with an anti-N-cadherin antibody, a higher gene transfer capability compared with the D-Ap layer was found in the N-cadherin-positive P19CL6 cells, but not in the N-cadherin-negative UV♀2 cells or in the P19CL6 cells that were pre-blocked with anti-N-cadherin. Therefore, the antigen-antibody binding that takes place at the cell-layer interface should be responsible for the higher gene transfer capability of the DA-Ap than D-Ap layer. These results suggest that the DA-Ap layer works as a mediator in a specific cell-targeted gene transfer system.

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“…As determined by a preliminary in vivo test, 58) the LAp-coated percutaneous implant successfully suppressed the host's foreign body response and integrated with the surrounding skin tissue that corresponds to a previously developed percutaneous device 59) made of apatite ceramics. Application studies of anti-infective percutaneous devices are currently in progress not only on this LAp coating but also on apatite coatings immobilizing antibacterial agents, 42) fibroblast growth factor-2, 38)-40) and/or ascorbate.…”

Section: Jcs-japanmentioning

confidence: 99%

“…34) Consequently, the content of the biomolecule immobilized in the apatite layer strongly correlates with the adsorption affinity between the biomolecule and apatite; the content increases with increasing adsorption affinity. 42) We have paid particular attention to the LAp layer and examined its physicochemical and biological properties in detail. As determined by transmission electron microscopy observation, the laminin molecules were not localized but dispersively immobilized in a matrix of apatite crystals on the nanoscale in the LAp layer (Fig.…”

Section: Biomimetic Process For Apatite Coatingmentioning

confidence: 99%

Development of apatite-based composites by a biomimetic process for biomedical applications

Oyane

2010

J. Ceram. Soc. Japan

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A new biomimetic process for apatite coating on polymeric materials has been developed. In this process, the surface of a polymer is modified with amorphous calcium phosphate (ACP), and then the polymer is immersed in a supersaturated calcium phosphate solution. The new biomimetic process has the advantages of safety, simplicity, and applicability to various types and forms of polymeric materials. By adding a biomolecule (such as protein, antibacterial agent, or DNA) to the supersaturated solution, immobilization of a biomolecule into the apatite coating while retaining the intrinsic biological activity of the biomolecule is possible. As a result of this, the base polymer would possess biological activity to control cell behaviors (such as adhesion, proliferation, and differentiation), in addition to good biocompatibility owing to the apatite. Hence, the new biomimetic process and the resulting composites have a wide variety of biomedical applications including tissue engineering scaffolds, percutaneous devices, and gene delivery carriers.

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The formation of an antibacterial agent–apatite composite coating on a polymer surface using a metastable calcium phosphate solution

Cited by 57 publications

References 28 publications

Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

Preliminary in vivo study of apatite and laminin‐apatite composite layers on polymeric percutaneous implants

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Development of apatite-based composites by a biomimetic process for biomedical applications

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