Development of apatite-based composites by a biomimetic process for biomedical applications

Oyane, Ayako

doi:10.2109/jcersj2.118.77

Cited by 32 publications

(24 citation statements)

References 55 publications

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“…The EVOH substrate was subjected to an alternate dipping treatment [26,28,30,31] that was simplified from the Taguchi's method [29]. First, the substrate was dipped in 20 ml of 200 mM aqueous solution of CaCl 2 (Nacalai Tesque, Japan) for 10 s, then dipped in 20 ml of ultrapure water for 1 s, and dried in air for a few minutes.…”

Section: Alternate Dipping Treatment For Pre-coating With Acpmentioning

confidence: 99%

“…apatite layers immobilizing both DNA and antibody molecules within them, and to evaluate their gene transfer efficiency for application in cell-targeted gene transfer. The DA-Ap layers were fabricated on an ethylene-vinyl alcohol copolymer (EVOH) substrate utilizing an amorphous calcium phosphate (ACP)-assisted biomimetic coating process [26]. In this process, the substrate was pre-coated with ACP nanoparticles and then immersed in a coating solution, i.e., a metastable supersaturated calcium phosphate solution (CP solution [27]) supplemented with DNA and an antibody.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Yazaki

Oyane

Araki

et al. 2012

Science and Technology of Advanced Materials

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Surface-mediated gene transfer systems using apatite (Ap)-based composite layers have received increased attention in tissue engineering applications owing to their safety, biocompatibility and relatively high efficiency. In this study, DNA-antibody-apatite composite layers (DA-Ap layers), in which DNA and antibody molecules are immobilized within a matrix of apatite nanocrystals, were fabricated using a biomimetic coating process. They were then assayed for their gene transfer capability for application in a specific cell-targeted gene transfer. A DA-Ap layer that was fabricated with an anti-CD49f antibody showed a higher gene transfer capability to the CD49f-positive CHO-K1 cells than a DNA-apatite composite layer (D-Ap layer). The antibody facilitated the gene transfer capability of the DA-Ap layer only to the specific cells that were expressing corresponding antigens. When the DA-Ap layer was fabricated with an anti-N-cadherin antibody, a higher gene transfer capability compared with the D-Ap layer was found in the N-cadherin-positive P19CL6 cells, but not in the N-cadherin-negative UV♀2 cells or in the P19CL6 cells that were pre-blocked with anti-N-cadherin. Therefore, the antigen-antibody binding that takes place at the cell-layer interface should be responsible for the higher gene transfer capability of the DA-Ap than D-Ap layer. These results suggest that the DA-Ap layer works as a mediator in a specific cell-targeted gene transfer system.

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Section: Alternate Dipping Treatment For Pre-coating With Acpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Yazaki

Oyane

Araki

et al. 2012

Science and Technology of Advanced Materials

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“…The apatite nucleation efficacy of the functional groups, such as Si-OH, is dependent not only on their composition but also on their kind [48], number [49], and arrangement [50]. Kokubo's group has demonstrated that various metal oxide gels, namely, silica (SiO 2 ) [51], titania (TiO 2 ) [52], zirconia (ZrO 2 ) [53], niobate (Nb 2 O 5 ) [54], and tantalum pentoxide (Ta 2 O 5 ) [55], prepared by sol-gel methods and then soaked in SBF, are able to form an apatite layer on their surfaces.…”

Section: Is a Functional Group Enough To Render Biomaterials Self-minmentioning

confidence: 99%

“…So, it has been proposed that such type of composite could be synthesized if the organic fibers are arranged in a 3D structure similar to that of collagen fibers in living bone and if they are modified to contain functional groups effective for apatite nucleation on their surface when soaking in SBF solution [71]. Oyane et al [49,50] successfully produced bioactive films textured on 3D template of polymers by coupling and hydrolysis of isocyanatopropyltriethoxysilane or sol-gel coupling of calcium silicate on ethylene-vinyl alcohol (EVOH) polymer. Balas et al [72,73] demonstrated that treating organic polymers, namely, polyethylene terephthalate (PET), EVOH, and Nylon 6, with a silane coupling agent and a titanium solution, induced the formation of bonelike apatite.…”

Section: Designing a Properly Functionalized Surfacementioning

confidence: 99%

Surfaces Inducing Biomineralization

Alves

Leonor

Azevedo

et al. 2012

Biomimetic Approaches for Biomaterials Development

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It is well known that there is a wide diversity of biological structural materials in Nature resulting from the intricate combination of inorganic minerals with organic polymers. Together, these are fashioned into a fascinating variety of shapes and forms. The study of these natural biomineralized structures has generated a growing awareness in materials science that the adaptation of biological processes may lead to significant advances in designing complex structures and controlling intricate processing routes that lead to the final shape. To date, it has not yet been possible to fully replicate these structures by nonbiological processing.Mineralized biological tissues are produced by living organisms of all kingdoms of life for different purposes such as body support, protection of vital organs, or defense against predators. They are highly optimized materials with outstanding properties and, moreover, these materials are produced at mild temperature and pressure conditions, with relatively low energy consumption, contrary to what typically occurs in synthetic materials. The majority of the organisms have their mineralized tissues composed of calcium phosphates and carbonates or amorphous silica.Bone is an example of a natural mineralized composite that has been extensively studied because of its unique structure and mechanical properties [1]. Bone is built according to a bottom-up approach, as typically occurs in these natural mineralized composites, in which the material is built starting at atomic and molecular scales, leading to the formation of nanostructured building blocks that in turn organize themselves in complex hierarchical structures [2]. The components at each level are related with each other, enabling the optimization of the performance for the required functions. Another characteristic of these natural composites is that the hard mineral component exhibits nanometric sizes, at least in one direction; displays an anisotropic geometry, and is immersed in a soft organic matrix. Gao [3] showed that the hierarchical organization, nanometer scale, and anisotropy of the mineral crystals are fundamental for the superior mechanical properties of these biocomposites, as evidenced in bone.

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“…Hydroxyapatite (HAp: Ca 10 (PO 4 ) 6 (OH) 2 ) granules and ceramics have been clinically applied for bioactive and substituted materials of hard tissues in dental and medical fields because of excellent biocompatibility and osteoconduction (Oyane, 2010;Yoshikawa, 2009;. In recent years, orthopedists and oral surgeons require the high capability of commercial HAp products for biomaterials, such as improvement of bio-absorption at implanted regions and early incorporation into bio-metabolic system (Artzi et al, 2004;Okuda et al, 2007;.…”

Section: Introductionmentioning

confidence: 99%

Microstructure and Biocompatibility of Hydroxyapatite Porous Ceramics Designed by a Partial Dissolution-Precipitation Technique with Supersonic Treatment for Bone Regeneration

Akazawa¹,

Murata²,

Tabata³

et al. 2012

Bone Regeneration

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Development of apatite-based composites by a biomimetic process for biomedical applications

Cited by 32 publications

References 55 publications

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Fabrication of DNA-antibody–apatite composite layers for cell-targeted gene transfer

Surfaces Inducing Biomineralization

Microstructure and Biocompatibility of Hydroxyapatite Porous Ceramics Designed by a Partial Dissolution-Precipitation Technique with Supersonic Treatment for Bone Regeneration

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