The formation of 2,5-dimethyl-4-hydroxy-2H-furan-3-one by cell-free extracts of Methylobacterium extorquens and strawberry (Fragaria×ananassa cv. Elsanta)

Koutsompogeras, Panagiotis; Kyriacou, Adamantini; Zabetakis, Ioannis

doi:10.1016/j.foodchem.2007.03.025

Cited by 19 publications

(5 citation statements)

References 18 publications

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“…Recently, an oxidoreductase, highly similar to strawberry and tomato enone oxidoreductase, capable of reducing α, β-unsaturated carbonyls, has been characterized from cucumber (Yamauchi et al 2011). Apart from the pathway contributed by the plant, biosynthesis of furaneol in strawberry has also been suggested to occur in collaboration with enzymes from an epiphytic bacterium, Methylobacterium extorquens (Zabetakis 1997; Koutsompogeras et al 2007; Verginer et al 2010; Zabetakis et al 2006). …”

Section: Introductionmentioning

confidence: 99%

An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls

et al. 2013

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Two furanones, furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone), are important constituents of flavor of the Alphonso cultivar of mango (Mangifera indica). To get insights into the biosynthesis of these furanones, we isolated an enone oxidoreductase gene from the Alphonso mango. It has high sequence similarity to an alkenal/one oxidoreductase from cucumber (79% identity) and enone oxidoreductases from tomato (73% identity) and strawberry (72% identity). The complete open reading frame was expressed in E. coli and the (his)6-tagged recombinant protein was purified by affinity chromatography. The purified protein assayed with NADH as a reducing agent converted D-fructose-1,6-diphosphate into furaneol, the immediate precursor of mesifuran. The enzyme was also able to convert two highly reactive carbonyls, 3-buten-2-one and 1-penten-3-one, produced by lipid peroxidation in plants, into their saturated derivatives. Expression profiling in various ripening stages of Alphonso fruits depicted an expression maxima at 10 days after harvest stage, shortly before the appearance of the maximum amount of furanones (completely ripe stage, 15 days after harvest). Although no furanones were detected at the 0 day after harvest stage, significant expression of this gene was detected in the fruits at this stage. Overall, the results suggest that this oxidoreductase plays important roles in Alphonso mango fruits.

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Section: Introductionmentioning

confidence: 99%

An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls

et al. 2013

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“…Methylotrophic bacteria induce formation of morphogenic calli and shoots of Triticum aestivum L., Nicotiana tabacum L., Solanum tuberosum L., and Linum usitatissimum L., and promote development of the regenerated plants (Kalyaeva et al 2001(Kalyaeva et al , 2003. Some Methylobacterium strains participate in biosynthesis of plant compounds (Zabetakis 1997;Koutsompogeras et al 2006) and others produce plant growth hormones such as cytokinins and auxins (Ivanova et al 2000Koenig et al 2002). Also the Scots pine endophytes produce adenine ribosides that can affect morphology and extend viability of bud explants in vitro (Pirttilä et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.)

Pirttilä

Podolich

Koskimäki

et al. 2008

Plant Cell Tiss Organ Cult

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Callus tissues originating from buds of mature Scots pine (Pinus sylvestris L.) trees exhibit the typical problem of browning, which leads to degeneration and death of the tissues. The effects of medium, origin (tree and location) and endophyte infection were studied on the browning and growth of bud-derived tissue cultures. The calli growing on medium with higher kinetin content and source of organic nitrogen, and originating from the southern location grew better and exhibited less browning. Endophytic microbial cells were detected in the brown callus tissues by transmission electron microscopy. The natural endophyte infection frequency of Scots pine buds was studied and found dependent on the tree, but not on the location. A well-growing, green callus line was artificially infected by an endophytic strain of Methylobacterium extorquens, and browning was not observed on solid media compared to the uninfected control clones of the same callus. However, suspension cultures started from the infected callus died faster than cultures started from the uninfected callus. The endophyte species composition and plant genotype together with tissue culture conditions are the key factors for gaining plant tissue cultures with high regeneration capacity.

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“…M. extorquens, was grown in a nutrient medium containing 0.75% (v/v) 1,2-propanediol, 0.25% (v/ v) methanol and 1.0% (w/v) peptone, as described previously (Koutsompogeras et al, 2007). Cells were harvested at the end of their exponential phase then centrifuged (using a Gallenkamp centrifuge) at 7000 rpm for 10 min, washed twice with 0.1 M phosphate buffer (pH 8.0) containing 2% w/v soluble polyvinylpyrrolidone (PVP) as a phenolic scavenger, 1 mM ZnSO 4 , 10 mM b-mercaptoethanol (BME) as a reducing agent, and the following protease inhibitors: 0.8 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM benzamidine hydrochloride (BHC), and 5 mM 3-amino-n-caproic acid (ACA).…”

Section: Organisms Cultivation and Cell Extract Preparationsmentioning

confidence: 99%

“…The formation of DMHF by cell-free extracts of Methylobacterium extorquens and strawberry (Fragaria x ananassa cv. Elsanta) was recently reported (Koutsompogeras, Kyriacou, & Zabetakis, 2007). The authors showed that no doubt existed on the cooperation between the plant and the bacterial cells in the formation of DMHF.…”

Section: Introductionmentioning

confidence: 96%

Characterization of NAD-dependent alcohol dehydrogenase enzymes of strawberry's achenes (Fragaria x ananassa cv. Elsanta) and comparison with respective enzymes from Methylobacterium extorquens

Koutsompogeras

Kyriacou

Zabetakis

2010

LWT - Food Science and Technology

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The formation of 2,5-dimethyl-4-hydroxy-2H-furan-3-one by cell-free extracts of Methylobacterium extorquens and strawberry (Fragaria×ananassa cv. Elsanta)

Cited by 19 publications

References 18 publications

An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls

An oxidoreductase from ‘Alphonso’ mango catalyzing biosynthesis of furaneol and reduction of reactive carbonyls

Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.)

Characterization of NAD-dependent alcohol dehydrogenase enzymes of strawberry's achenes (Fragaria x ananassa cv. Elsanta) and comparison with respective enzymes from Methylobacterium extorquens

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