Characterization of NAD-dependent alcohol dehydrogenase enzymes of strawberry's achenes (Fragaria x ananassa cv. Elsanta) and comparison with respective enzymes from Methylobacterium extorquens

Koutsompogeras, Panagiotis; Kyriacou, Adamantini; Zabetakis, Ioannis

doi:10.1016/j.lwt.2010.01.017

Cited by 6 publications

(5 citation statements)

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“…A previous study revealed that the molecular weight of ADH was 24.6 kDa and the optimum pH was 6 for ADH activity in strawberry callus cultures . The appropriate substrates of ADH included 1‐propanol (Michaelis constant (Km) = 3.54 mmol L −1 ) and ethanol (Km = 6.66 mmol L −1 ) . The characteristics of ADH in strawberry achenes differed depending on callus culture.…”

Section: Characteristic Volatile Compounds In Strawberrymentioning

confidence: 99%

“…The characteristics of ADH in strawberry achenes differed depending on callus culture. The optimum substrates for achene ADH were ethanol (Km = 5.950 mmol L −1 ) and methanol (Km = 12.610 mmol L −1 ) . The ADH gene was the first protein‐encoding gene completely sequenced in strawberries .…”

Section: Characteristic Volatile Compounds In Strawberrymentioning

confidence: 99%

“…41 The appropriate substrates of ADH included 1-propanol (Michaelis constant (Km) = 3.54 mmol L −1 ) and ethanol (Km = 6.66 mmol L −1 ). 42 The characteristics of ADH in strawberry achenes differed depending on callus culture. The optimum substrates for achene ADH were ethanol (Km = 5.950 mmol L −1 ) and methanol (Km = 12.610 mmol L −1 ).…”

Section: Estersmentioning

confidence: 99%

“…The optimum substrates for achene ADH were ethanol (Km = 5.950 mmol L −1 ) and methanol (Km = 12.610 mmol L −1 ). 42 The ADH gene was the first protein-encoding gene completely sequenced in strawberries. 43 This gene and its intron-containing sequence were commonly applied in ongoing phylogenetic studies on Fragaria species.…”

Section: Estersmentioning

confidence: 99%

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The aroma volatile repertoire in strawberry fruit: a review

et al. 2018

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Aroma significantly contributes to flavor, which directly affects the commercial quality of strawberries. The strawberry aroma is complex as many kinds of volatile compounds are found in strawberries. In this review, we describe the current knowledge of the constituents and of the biosynthesis of strawberry volatile compounds, and the effect of postharvest treatments on aroma profiles. The characteristic strawberry volatile compounds consist of furanones, such as 2,5-dimethyl-4-hydroxy-3(2H)-furanone and 4-methoxy-2,5-dimethyl-3(2H)-furanone; esters, including ethyl butanoate, ethyl hexanoate, methyl butanoate, and methyl hexanoate; sulfur compounds such as methanethiol, and terpenoids including linalool and nerolidol. As for postharvest treatment, the present review discusses the overview of aroma volatiles in response to temperature, atmosphere, and exogenous hormones, as well as other treatments including ozone, edible coating, and ultraviolet radiation. The future prospects for strawberry volatile biosynthesis and metabolism are also presented. © 2018 Society of Chemical Industry.

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Section: Characteristic Volatile Compounds In Strawberrymentioning

confidence: 99%

Section: Characteristic Volatile Compounds In Strawberrymentioning

confidence: 99%

Section: Estersmentioning

confidence: 99%

Section: Estersmentioning

confidence: 99%

See 2 more Smart Citations

The aroma volatile repertoire in strawberry fruit: a review

et al. 2018

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“…Sets of ADH genes or ADH-like genes have been identified in the genomes of poaceae, rosaceae, brassicaceae, fabaceae, and pinaceae plants ( Thompson et al, 2010 ; Zheng et al, 2011 ). So far, most members of the ADHs in plants characterized at the gene level belonged to the medium-chain ADH protein superfamily (including ADH1, EC 1.1.1.1 and FDH, Class III ADH, EC 1.2.1.1; Chase, 1999 ), which usually contains zinc ligands in their active site ( Tesnière and Verriès, 2000 ; Garabagi et al, 2005 ; ManrÍquez et al, 2006 ; Koutsompogeras et al, 2010 ; Singh et al, 2010 ; Komatsu et al, 2011 ; Pathuri et al, 2011 ; Bukh et al, 2012 ; Iaria et al, 2012 ; Min et al, 2012 ; Cheng F.F. et al, 2013 ), and the short-chain ADH protein superfamily, which lacks zinc-liganding cysteine residues in their coenzyme binding regions, and the molecular functions of only a few short-chain ADH genes were known ( ManrÍquez et al, 2006 ; Gonzalez-Agüero et al, 2009 ; Kim et al, 2009 ; Strommer, 2011 ; Moummou et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

The Alcohol Dehydrogenase Gene Family in Melon (Cucumis melo L.): Bioinformatic Analysis and Expression Patterns

et al. 2016

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Alcohol dehydrogenases (ADH), encoded by multigene family in plants, play a critical role in plant growth, development, adaptation, fruit ripening and aroma production. Thirteen ADH genes were identified in melon genome, including 12 ADHs and one formaldehyde dehydrogenease (FDH), designated CmADH1-12 and CmFDH1, in which CmADH1 and CmADH2 have been isolated in Cantaloupe. ADH genes shared a lower identity with each other at the protein level and had different intron-exon structure at nucleotide level. No typical signal peptides were found in all CmADHs, and CmADH proteins might locate in the cytoplasm. The phylogenetic tree revealed that 13 ADH genes were divided into three groups respectively, namely long-, medium-, and short-chain ADH subfamily, and CmADH1,3-11, which belongs to the medium-chain ADH subfamily, fell into six medium-chain ADH subgroups. CmADH12 may belong to the long-chain ADH subfamily, while CmFDH1 may be a Class III ADH and serve as an ancestral ADH in melon. Expression profiling revealed that CmADH1, CmADH2, CmADH10 and CmFDH1 were moderately or strongly expressed in different vegetative tissues and fruit at medium and late developmental stages, while CmADH8 and CmADH12 were highly expressed in fruit after 20 days. CmADH3 showed preferential expression in young tissues. CmADH4 only had slight expression in root. Promoter analysis revealed several motifs of CmADH genes involved in the gene expression modulated by various hormones, and the response pattern of CmADH genes to ABA, IAA and ethylene were different. These CmADHs were divided into ethylene-sensitive and –insensitive groups, and the functions of CmADHs were discussed.

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