The forkhead box O transcription factor regulates lipase and anti-microbial peptide expressions to promote lipid catabolism and improve innate immunity in the Eriocheir sinensis with hepatopancreatic necrosis disease

Zhao, Yuqi; Nie, Ximei; Han, Zhengxiao; Liu, Peng; Xu, Hao; Huang, Xin; Ren, Qian

doi:10.1016/j.fsi.2022.03.047

Cited by 10 publications

(5 citation statements)

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“…For instance, FOXO1 and FOXO3α are expressed in multiple tissues, while FOXO4 is predominantly expressed in the kidney and muscle, and FOXO6 exhibits high expression in the liver [9]. Our results showed that the EsFOXO-like transcripts were expressed in various tissues, such as hepatopancreas, hemocytes, heart, stomach, gills, muscles, and eyestalks, with the highest level of expression in the hepatopancreas, which is similar to the distribution of FOXO genes in crustaceans [3,19]. The hepatopancreas of crustaceans has been identified to serve as a vital immunologic and metabolic organ [63].…”

Section: Discussionsupporting

confidence: 71%

“…The ORF of EsFOXOlike was 852 bp and encoded a 283 amino acid polypeptide. The EsFOXO-like sequence perfectly contains a typical Forkhead (FH) domain [19]. The DBD domain, also named the FH domain, was found to be highly conserved in different species [60,61].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, many studies have demonstrated that FOXO regulates molting and metamorphosis in insects [16]. Although FOXO has been identified in several crustacean species, most studies only focus on its regulatory role in the immune response against bacterial invasions [3,19]. Thus, the potential involvement of FOXO in growth still needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on the physiological role of FOXO in crustaceans are mainly focused on the immune response. It has been reported that the expression of FOXO was significantly decreased in the intestine of crabs after hepatopancreatic necrosis disease (HPND) stimulation [19]. In the Chinese mitten crab, FOXO has also been found to have a positive impact on the expression levels of genes coding antimicrobial peptides (AMPs) [19].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that the expression of FOXO was significantly decreased in the intestine of crabs after hepatopancreatic necrosis disease (HPND) stimulation [19]. In the Chinese mitten crab, FOXO has also been found to have a positive impact on the expression levels of genes coding antimicrobial peptides (AMPs) [19]. In Marsupenaeus japonicas, FOXO was discovered to upregulate the expression of AMPs via the IMD pathway as well as enhance the phagocytosis of hemocytes against pathogenic bacteria [1,3].…”

Section: Introductionmentioning

confidence: 99%

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FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

Yang

et al. 2023

JMSE

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The Forkhead Box O (FOXO) gene plays a key role in various biological processes, such as growth, metabolism, development, immunity and longevity. Molting is an essential process for crustacean growth, which is mainly regulated by 20-hydroxyecdysone (20E) and molt-inhibiting hormone (MIH). Although the role of FOXO in regulating the immune response of crustaceans is well documented, its involvement in controlling crustacean molting remains unclear. In this study, a FOXO-like gene (designed as EsFOXO-like) was identified in Eriocheir sinensis, and the regulation of the 20E pathway by EsFOXO-like was also investigated. The coding sequence of EsFOXO-like was 852 bp, which consisted of 283 amino acids including a conserved Forkhead (FH) domain. EsFOXO-like shared high similarity with FOXO genes from other crustaceans, and the mRNA expression levels of the EsFOXO-like gene were highest in the hepatopancreas and lowest in the hemocytes. However, transcription and protein expression of the EsFOXO-like gene were found to be up-regulated only during the pre-molt stage in the hepatopancreas, with lower expression levels observed at the post-molt stage. To explore the role of EsFOXO-like in the 20E pathway, EsFOXO-like was firstly inhibited by a specific FOXO inhibitor (AS1842856) and then through an EsFOXO-like dsRNA injection, respectively, and the results showed that the relative expression levels of EsFOXO-like were notably decreased in the hepatopancreas after both the inhibitor and dsRNA treatments. The 20E concentration, the mRNA expression levels of the 20E receptors including the ecdysone receptor (EcR) and the retinoid-X receptor (RXR) and EsmTOR transcription in the AS1842856 group or the EsFOXO-RNAi group were all significantly higher than that in the control group, while the mRNA expression level of EsMIH was significantly decreased after EsFOXO-like inhibition. To further investigate whether the EsFOXO-like acts through mTOR or not, Rapamycin was administered to inhibit mTOR activity in EsFOXO-like inhibited crabs. The results revealed a significant reduction in the concentration of 20E and the expression level of EsMIH in the AS1842856 + Rapamycin group compared to the AS1842856 + DMSO group, accompanied by an increase in EsEcR and EsRXR expression. These findings collectively suggest that EsFOXO-like regulates the 20E pathway through mTOR, which offered valuable insights into the understanding of the molting process in crustaceans.

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