Affecting mechanism of Chlorella sorokiniana meal replacing fish meal on growth and immunity of Litopenaeus vannamei based on transcriptome analysis

Xu, Zhihua; Li, Menglu; Wang, Yuanyuan; Feng, Miaolin; Gan, Zhending; Leng, Xiangjun; Li, Xiaoqin

doi:10.1016/j.aqrep.2023.101645

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…To date, research has also been conducted on substituting FM with CHL and CAP in L. vannamei. Xu et al found that a 60% substitution of FM with CHL resulted in a significant increase in the growth performance of L. vannamei and promoted significant enrichment of its protein digestion and lysosomal pathways [35]. Jiang et al [36] and Yao et al [37] discovered that substituting a moderate quantity of FM with CAP in the diet increased the nonspecific immune-related enzyme activities of L. vannamei and affected a number of pathways in the muscle and hepatopancreas, including protein digestion and absorption, pancreatic secretion, amino acid and fatty acid metabolism, and ribosomal pathways.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Three Protein Sources Used in Medium-Sized Litopenaeus vannamei: Effects on Growth, Immunity, Intestinal Digestive Enzyme Activity, and Microbiota Structure

Yuan,

Hu,

Zheng

et al. 2023

Fishes

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The type of protein source in diets has many effects on shrimp. In this study, Litopenaeus vannamei with an initial body weight of 3.68 ± 0.002 g were fed for 8 weeks on three experimental diets (isoproteic: 41.00%; isolipidic: 7.61%) that were formulated using fish meal (FM), Chlorella sorokiniana (CHL), and Clostridium autoethanogenum protein (CAP) as the primary protein sources, respectively. This study examined the growth, non-specific immunity, intestinal digestion, and microbiota of L. vannamei after the feeding experiment concluded. Compared to the FM group, the findings indicate that the weight gain rate (WGR), specific growth rate (SGR), and protein efficiency ratio (PER) of L. vannamei were notably enhanced via dietary CAP. The CHL group exhibited the highest levels of catalase (CAT), phenoloxidase (PO), and superoxide dismutase (SOD) activities in the gills of L. vannamei, whereas the FM group had the lowest levels. Conversely, the malondialdehyde (MDA) content showed the opposite trend. Both dietary CHL and CAP promoted the digestive enzyme activities of L. vannamei, with dietary CAP having a more pronounced promotional effect. An analysis of alpha diversity indicated that the consumption of dietary CHL substantially enhanced the abundance and diversity of microbiota in the intestinal tract of L. vannamei. Furthermore, the dietary CHL significantly increased the colonization of immune-associated beneficial bacteria and inhibited the colonization of pathogenic bacteria in the intestinal tract of L. vannamei, whereas dietary CAP mainly increased the colonization of growth-associated beneficial bacteria. Functional predictions showed that different dietary protein sources affect various metabolic activities and signaling pathways of L. vannamei, and some functions, including signal transduction, cell motility, and the immune system, were significantly enhanced in the CHL group. In summary, both dietary CHL and dietary CAP promoted growth and immunity in L. vannamei compared to dietary FM. The results of this study could be helpful for the sustainable development of shrimp farming.

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Section: Introductionmentioning

confidence: 99%

A Comparison of Three Protein Sources Used in Medium-Sized Litopenaeus vannamei: Effects on Growth, Immunity, Intestinal Digestive Enzyme Activity, and Microbiota Structure

Yuan,

Hu,

Zheng

et al. 2023

Fishes

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Effective valorization of Chlorella biomass and Brewers spent grain substituting fish meal and soybean meal in the diet of herbivorous fish Cirrhinus reba for higher growth, digestibility and sustainable cultivation

Chattaraj,

Behera,

Das Mohapatra

2023

J Appl Phycol

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Effects of Substituting Fish Meal by Chlorella Meal in Practical Diet on Growth, Feed Utilization, and Flesh Quality of Pacific White Shrimp (Penaeus vannamei)

Li,

Yao

et al. 2024

Aquaculture Nutrition

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This study investigated the effects of substituting fish meal (FM) in practical diet by chlorella meal on the growth, feed utilization, and flesh quality of Pacific white shrimp (Penaeus vannamei). First, a basal diet was prepared with 200 g/kg FM inclusion (FM‐20), and then chlorella meal was used to reduce FM inclusion to 150 g/kg (FM‐15), 100 g/kg (FM‐10), 50 g/kg (FM‐5), and 0 g/kg (FM‐0), corresponding to the replacement levels of 25%, 50%, 75%, and 100% of dietary FM, respectively. Shrimp (1.37 ± 0.10 g) were fed with the five isonitrogenous and isolipidic diets for 56 days. No significant difference was observed in feed conversion ratio (FCR) and weight gain (WG) between FM‐20 and FM‐15 group (P > 0.05), but when chlorella meal substituted 50% of dietary FM, WG, protein and lipid retention, and n‐3/n‐6 PUFAs in flesh were significantly reduced with significant increase in FCR (P < 0.05). Survival, feed intake, meat yield, apparent digestibility coefficient of crude protein, dry matter, and flesh shear force showed no significant difference between FM‐20 and substituted groups (P > 0.05). When dietary FM was totally substituted by chlorella meal, the body yellowness and redness and essential amino acid content in flesh, including Lys and Met, were significantly reduced (P < 0.05). No significant differences were found in flesh total collagen, crude lipid, crude protein, serum biochemical indexes, flesh texture profiles (hardness, springiness, etc.), water holding capacity, antioxidant capacity, fatty acid, free amino acid composition, and muscle fiber density among the five treatments (P > 0.05). To sum up, in a practical diet with 200 g/kg FM inclusion, chlorella meal successfully replaced 25% of dietary FM without adverse impacts on the growth and feed utilization, and the substitution of 75% of dietary FM did not negatively affect the flesh quality of Pacific white shrimp.

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Affecting mechanism of Chlorella sorokiniana meal replacing fish meal on growth and immunity of Litopenaeus vannamei based on transcriptome analysis

Cited by 3 publications

References 53 publications

A Comparison of Three Protein Sources Used in Medium-Sized Litopenaeus vannamei: Effects on Growth, Immunity, Intestinal Digestive Enzyme Activity, and Microbiota Structure

A Comparison of Three Protein Sources Used in Medium-Sized Litopenaeus vannamei: Effects on Growth, Immunity, Intestinal Digestive Enzyme Activity, and Microbiota Structure

Effective valorization of Chlorella biomass and Brewers spent grain substituting fish meal and soybean meal in the diet of herbivorous fish Cirrhinus reba for higher growth, digestibility and sustainable cultivation

Effects of Substituting Fish Meal by Chlorella Meal in Practical Diet on Growth, Feed Utilization, and Flesh Quality of Pacific White Shrimp (Penaeus vannamei)

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