The fluorescence detection of glutathione by ∙OH radicals’ elimination with catalyst of MoS2/rGO under full spectrum visible light irradiation

Zhang, Nan; Ma, Weiguang; Han, Dongxue; Wang, Lingnan; Wu, Tongshun; Niu, Li

doi:10.1016/j.talanta.2015.07.003

Cited by 39 publications

(9 citation statements)

References 65 publications

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“…9b, inset). According to the 3σ rule, the LOD of GSH was calculated to be 0.5043 μM, which is comparable or lower than that of some reported methods, including fluorimetry [57][58][59] and electrochemistry [60]. From , and Cl − , respectively.…”

Section: Robustness Of Peroxidase Activity Of Nis/mmt/go Nanocompositesmentioning

confidence: 52%

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Chen

Zhu

Yang

et al. 2018

Adv Compos Hybrid Mater

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In the present study, a facile method was proposed to prepare nickel sulfide (NiS) nanoparticles well-dispersed on the surface of montmorillonite/graphene oxide (MMT/GO). The NiS/MMT/GO nanocomposites were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), fluorescence spectrophotometry, etc. Significantly, the novel NiS/MMT/GO nanocomposites exhibited high peroxidase-like activity toward the typical chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). However, glutathione (GSH) could inhibit the peroxidase-like activity of NiS/ MMT/GO along with a visible color variation. Based on the color change of the system of nanocomposites-H 2 O 2 -TMB, a novel colorimetric sensor was designed and conveniently used to quantitatively detect H 2 O 2 as well as GSH. The limit of detection (LOD) of H 2 O 2 and GSH is 9.73 and 0.5043 μM, respectively. The proposed H 2 O 2 sensor has potential application in the field of biosensing, food, and environment.

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Section: Robustness Of Peroxidase Activity Of Nis/mmt/go Nanocompositesmentioning

confidence: 52%

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Chen

Zhu

Yang

et al. 2018

Adv Compos Hybrid Mater

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“…Different Mo compounds in nanosized forms were tested on mammalian cell lines in various in vitro experimental setups. Human cell lines, such as MCF-7 (breast cancer) important role in organism homeostasis, e.g., H 2 O 2 or glutathione [26,27]. Singh et al [28] have created a biosensor allowing fluorescent pathogen detection.…”

Section: Biological Effects Of Nanoparticles Of Mo Compounds Basic Cymentioning

confidence: 99%

Biological effects of molybdenum compounds in nanosized forms under <i>in vitro</i> and <i>in vivo</i> conditions

Sobańska¹,

Zapór²,

Szparaga³

et al. 2020

Int J Occup Med Environ Health

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Nanoparticles of transition metal dichalcogenides, particularly of molybdenum (Mo), have gained a lot of focus due to their exceptional physicochemical properties and the growing number of technological applications. These nanoparticles are also considered as potential therapeutic tools, biosensors or drug carriers. It is crucial to thoroughly examine their biocompatibility and ensure safe usage. The aim of this review is to analyze the available data on the biological effects of different nanoforms of elemental Mo and its compounds. In the reviewed publications, different conditions were described, including different experimental models, examined nanoforms, and their used concentrations. Due to these differences, the results are rather difficult to compare. Various studies classify Mo related nanomaterials as very toxic, mildly toxic or non-toxic. Similarly, the mechanisms of toxicity proposed in some studies are different, including oxidative stress induction, physical membrane disruption or DNA damage. Quite promising, however, are the potential medical applications of MoS 2 nanoparticles in therapy of cancer and Alzheimer's disease. Further studies on biocompatibility of nanomaterials based on Mo compounds are warranted.

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“…We used glutathione (GSH) as a hydroxyl radical scavenger to further verify the generation of • OH according to the previous report. 19 When GSH is introduced into the system, it is able to capture and eliminate • OH radicals as a competitor to TA, which will decrease the fluorescent response of TAOH. With the introduction of GSH, the fluorescence intensity was observed to decrease as expected (Figure S1C).…”

Section: T H Imentioning

confidence: 99%

When Fluorescent Sensing Meets Electrochemical Amplifying: A Powerful Platform for Gene Detection with High Sensitivity and Specificity

et al. 2021

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Ultrasensitive and ultraselective detection of the gene requires emergency development to meet the medical demands and infectious disease control. Herein we report a versatile and scalable method based on electrochemical-chemical-cyclic amplification (EC-CA) and fluorescence detection for ultrasensitive gene sensing. The EC-CA is achieved by an electro-Fenton reaction (EFR). The hydroxyl radicals generated at EFR are trapped by terephthalic acid to form highly fluorescent 2-hydroxyterephthalic acid, which can be sensitively detected by a fluorescence spectrophotometer. The method is the first to be able to amplify the signal and reduce the noise simultaneously by using the conventional analytical methods directly. This described method can be used for reliable Fe3+ quantification in the range from 0.1 nM to 0.08 mM. The calculated limit of detection (LOD) is 0.02 nM. Then, hepatitis B virus (HBV) and p53 gene were detected by this proposed method through introducing the Fe3O4 nanoparticles into the gene hybridization system. The LODs for HBV and p53 gene even topped out at 2.6 pM and 1.7 fM, respectively. We demonstrated that the finally recorded signal was triply amplified through the EC cycle, Fe3O4 nanoparticles, and sensitive fluorescence detection. At the same time, the background signal arisen from matrix effects and readout noise was effectively suppressed. This method shows it is simple, convenient, and operational through the detection of Fe3+, HBV, and the p53 gene in blood samples, respectively. We believe our method will make a significant, near-term impact on the development of high-sensitivity methods that are versatile and scalable.

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The fluorescence detection of glutathione by ∙OH radicals’ elimination with catalyst of MoS2/rGO under full spectrum visible light irradiation

Cited by 39 publications

References 65 publications

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Novel synthesis of NiS/MMT/GO nanocomposites with enhanced peroxidase-like activity for sensitive colorimetric detection of glutathione in solution

Biological effects of molybdenum compounds in nanosized forms under <i>in vitro</i> and <i>in vivo</i> conditions

When Fluorescent Sensing Meets Electrochemical Amplifying: A Powerful Platform for Gene Detection with High Sensitivity and Specificity

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