The Fissiparous Race of the Planarian<i>Dugesia Lugubris S.L.</i>Found in Barcelona (Spain) Belongs to the Biotype G: Comparative Analysis of the Karyotypes

Benazzi, Mario; Ballester, Rafael; Baguñà, Jaume; Puccinelli, Ileana

doi:10.1080/00087114.1972.10796465

Cited by 24 publications

(11 citation statements)

References 3 publications

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“…Clonal line CIW4 (Sánchez Alvarado et al, 2002) of the diploid, asexual strain of Schmidtea mediterranea (Benazzi et al, 1972) was used for all experiments. Animals were maintained at 20°C as previously described (Newmark and Sánchez Alvarado, 2000).…”

Section: Methodsmentioning

confidence: 99%

Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea

Oviedo

Newmark

Alvarado

2003

Developmental Dynamics

155

142

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The regulation of scale and proportion in living organisms is an intriguing and enduring problem of biology. Regulatory mechanisms for controlling body size and proportion are clearly illustrated by the regeneration of missing body parts after amputation, in which the newly regenerated tissues ultimately attain a size that is anatomically congruent with the size of the rest of the organism. Understanding the molecular processes underpinning scaling would have deep consequences for our comprehension of tissue regeneration, developmental ontogeny, growth, and evolution. Although many theories have been put forward to explain this process, it is interesting that no satisfactory mechanistic explanation is currently available to explain scalar relationships. We chose to investigate the freshwater planarian, a commonly used model system for the study of metazoan regeneration, to delineate a strategy for the molecular dissection of scale and proportion mechanisms in metazoans. Here, we report on the cloning and discrete expression pattern of a novel planarian gene, which shares homology with the DEG/ENaC super-family of sodium channels. We have named H.112.3c cintillo ("head ribbon" in Spanish) and present a strategy for using the expression of this gene to monitor scale and proportion regulation during regeneration, growth and degrowth in the freshwater planarian Schmidtea mediterranea. Developmental Dynamics 226:326 -333, 2003.

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Section: Methodsmentioning

confidence: 99%

Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea

Oviedo

Newmark

Alvarado

2003

Developmental Dynamics

155

142

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“…Asexual strains of the freshwater flatworm Schmidtea mediterranea (Benazzi et al 1972) were maintained at the dark in a climatised room (20°C) in double distilled water with 1.6 mM NaCl, 1.0 mM CaCl 2 , 1.0 mM MgSO 4 , 0.1 mM MgCl 2 , 0.1 mM KCl and 1.2 mM NaHCO 3 . In order to generate genetically identical animals, strains were created by serially amputating individual worms and allowing the fragments to regenerate.…”

Section: Schmidtea Mediterraneamentioning

confidence: 99%

Reference genes for qPCR assays in toxic metal and salinity stress in two flatworm model organisms

et al. 2011

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The flatworm species Schmidtea mediterranea and Macrostomum lignano have become new and innovative model organisms in stem cell, regeneration and tissue homeostasis research. Because of their unique stem cell system, (lab) technical advantages and their phylogenetic position within the Metazoa, they are also ideal candidate model organisms for toxicity assays. As stress and biomarker screenings are often performed at the transcriptional level, the aim of this study was to establish a set of reference genes for qPCR experiments for these two model organisms in different stress situations. We examined the transcriptional stability of nine potential reference genes (actb, tubb, ck2, cox4, cys, rpl13, gapdh, gm2ap, plscr1) to assess those that are most stable during altered stress conditions (exposure to carcinogenic metals and salinity stress). The gene expression stability was evaluated by means of geNorm and NormFinder algorithms. Sets of best reference genes in these analyses varied between different stress situations, although gm2ap and actb were stably transcribed during all tested combinations. In order to demonstrate the impact of bad normalisation, the stress-specific gene hsp90 was normalised to different sets of reference genes. In contrast to the normalisation according to GeNorm and NormFinder, normalisation of hsp90 in Macrostomum lignano during cadmium stress did not show a significant difference when normalised to only gapdh. On the other hand an increase of variability was noticed when normalised to all nine tested reference genes together. Testing appropriate reference genes is therefore strongly advisable in every new experimental condition.

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“…A clonal line of the diploid, asexual strain of Schmidtea mediterranea (Benazzi et al, 1972) was used. Animals were maintained at 18°C in 1ϫ Montjuïch salts (1.6 mmol/l NaCl, 1.0 mmol/l CaCl 2 , 1.0 mmol/l MgSO 4 , 0.1 mmol/l MgCl 2 , 0.1 mmol/l KCl and 1.2 mmol/l NaHCO 3 prepared in Milli-Q water).…”

Section: Organismsmentioning

confidence: 99%

Planarian homologs ofnetrinandnetrin receptorare required for proper regeneration of the central nervous system and the maintenance of nervous system architecture

2005

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The Fissiparous Race of the PlanarianDugesia Lugubris S.L.Found in Barcelona (Spain) Belongs to the Biotype G: Comparative Analysis of the Karyotypes

Cited by 24 publications

References 3 publications

Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea

Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea

Reference genes for qPCR assays in toxic metal and salinity stress in two flatworm model organisms

Planarian homologs ofnetrinandnetrin receptorare required for proper regeneration of the central nervous system and the maintenance of nervous system architecture

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