The first radiation hybrid map of a perch-like fish: The gilthead seabream (Sparus aurata L)

Abstract

Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RH panel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a cost-effective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

“…Comparison between sea bream linkage and the RH map: A preliminary RH map for the gilthead sea bream has been recently reported (Senger et al 2006). It contains 440 markers, including 121 microsatellite loci that have been mapped onto the linkage map presented here.…”

“…The great importance of the gilthead sea bream for marine aquaculture has fueled an increasing number of studies in many different areas such as immunology, endocrinology, bone morphology, and muscle physiology. Moreover, the genomic tool kit for S. aurata has been constantly improving, as a firstgeneration cDNA microarray was recently reported (Sarropoulou et al 2005), a preliminary radiation hybrid (RH) map has been constructed (Senger et al 2006), and a medium-scale expressed sequence tag (EST) sequencing project has been recently completed (A. Canario, personal communication). An improved RH map with .1000 markers (G. Kotoulas, personal communication) and an oligo array representing .10,000 unique transcripts (L. Bargelloni, personal communication) are expected by the end of 2006.…”

Construction of Genetic Linkage Maps and Comparative Genome Analysis of Catfish Using Gene-Associated Markers

“…To date, no whole-genome sequence exists for any aquaculture species. Major progress, however, has been made in the generation of other genome resources for some economically important aquaculture species such as tilapia (Katagiri et al 2005;Lee et al 2005;Ferreira and Martins 2008), rainbow trout (Rexroad and Palti 2003;Guyomard et al 2006Guyomard et al , 2007, Atlantic salmon (Moen et al 2004(Moen et al , 2008, gilthead seabream (Sparus aurata) Senger et al 2006;Sarropoulou et al 2008), and the European sea bass (Dicentrarchus labrax) (Chistiakov et al 2005;Whitaker et al 2006).…”

Food and Feeding Habits of Red Lionfish Pterois volitans from Cuddalore Coast, South East Coast Construction of Radiation Hybrid Panels for Two Major Aquaculture Species: Sturgeon and Oyster

“…Splenocytes can be obtained in very large numbers, ready to use after cutting the whole organ into pieces with scissors and gentle dissociation in a Potter device. Fusion between fish splenocytes and hamster cells was straightforward: we have been able to produce hybrid cells with all the species we have tested so far, including seabream [6], seabass [7], tilapia [8], sturgeon (this work) and trout (unpublished data). For oyster RH lines, once we had overcome the technical issue of the difference in the osmotic pressures appropriate for oyster cells and the hamster cells, we had no major difficulty in obtaining enough donor cells from oyster muscle.…”

“…Conversely cells prepared from non-adapted oysters did not survive when subjected to the same conditions. Sturgeon and oyster cells prepared as described were irradiated with a dose of 3500 rads, which past experience suggests is suitable for constructing panels of manageable resolution [6][7][8]12,13]. Following fusions, the cells were incubated in HAT medium to allow the growth of the hybrid cells only.…”