The first isolation and identification of canine parvovirus (CPV) type 2c variants during 2016–2018 genetic surveillance of dogs in Mongolia

Temuujin, Uyangaa; Tserendorj, Ariunaa; Fujiki, Jumpei; Sakoda, Yoshihiro; Erdene-Ochir, Tseren-Ochir; Okamatsu, Masatoshi; Matsuno, Keita; Sharav, Tumenjargal; Horiuchi, Motohiro; Umemura, Takashi; Chultemdorj, Tungalag

doi:10.1016/j.meegid.2019.05.006

Cited by 15 publications

(15 citation statements)

References 33 publications

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“…Among 146 strain of CPV-2b, 116 strain had the same complete sequence for VP2. CPV-2c strains are currently circulating in East and South-East Asian countries [16][17][18][19][20][21][22]. However, we demonstrated that none of the dogs in Japan was infected with CPV-2c.…”

Section: Discussionmentioning

confidence: 72%

“…Similarly, an epidemiological study in Laos and Vietnam demonstrated that over 90% of infected dogs had the CPV-2c variant [17, 18]. CPV-2c was identi ed in various countries in Asia except in Japan [15][16][17][18][19][20][21][22]. Collectively, these studies suggest that the composition of antigenic variants in Japan is rare compared to other neighboring countries.…”

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confidence: 99%

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Predominance of canine parvovirus 2b in Japan: an epidemiological study during 2014-2019

Takano

Hamaguchi²,

Hasegawa³

et al. 2021

Arch Virol

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Canine parvovirus-2 (CPV-2) is an important pathogen of domestic dogs and wild canids. In Japan, CPV-2 infection remains one of the most common infection diseases among dogs. We analyzed samples collected between 2014 and 2019 to determine the antigenic variants of CPV-2 among dogs in Japan. Our results demonstrated that the CPV-2b variant was predominant. The CPV-2c variant was not found among our samples. Our ndings demonstrate that the distribution of CPV-2 antigenic variants in Japan was more similar to the distribution in Australia compared with that of neighboring countries in Asia. IntroductionCanine parvovirus-2 (CPV-2) is an important pathogen of domestic dogs and wild canids. CPV-2 is small non-enveloped virus with a single-stranded DNA genome, and classi ed into the family Parvoviridae, subfamily Parvovirinae, genus Protoparvovirus, species Carnivore protoparvovirus-1 [1]. The CPV-2 genome encodes for 4 proteins: NS1, NS2, VP1, and VP2. NS1 and NS2 are non-structural proteins that regulate replication of the viral genome, while VP1 and VP2 are structural proteins that constitute capsids of the virion [2,3]. Amino acid residue in VP2 de nes the antigenic variants of CPV-2 [3]. CPV-2 infection is characterized by gastroenteritis-like symptoms such as fever, anorexia, diarrhea, and vomiting [4]. Unvaccinated adult dogs usually have subclinical infection [5].CPV-2 was identi ed in the late 1970s in dogs [6]. CPV-2 is genetically similar to other carnivore parvoviruses, and presumably derived from feline panleukopenia virus (FPLV) [7]. Within a few years after the outbreak of CPV-2 infection across the United States and Australia in 1978, CPV-2 infection was identi ed in dogs across a number of countries. In the 1980s, two antigenic variants of CPV-2, namely CPV-2a and CPV-2b, emerged [8]. In 2000, a third variant, CVP-2c, was detected in dogs in Italy [9, 10]. These three antigenic variants have a different amino acid in residue 426 of VP2 protein (CPV-2a: N, CPV-2b: D, CPV-2c: E) [8,10,11]. Epidemiological studies over the past 20 years have identi ed these three antigenic variants in the dog population. However, the rst CPV-2 (original CPV-2) has virtually disappeared in the dog population. Studies suggest that there is no difference among the antigenic variants in terms of pathogenicity [12]. However, the licensed CPV2 vaccine is thought to be less effective in protecting dogs against CPV-2c [13].An epidemiological study by Soma et al. [14] demonstrated that CPV-2b was the dominant antigenic variant among dogs in Japan between 2009 and 2011. However, a recent study demonstrated that other antigenic variants are more common in other Asian countries that are geographically close to Japan. For example, the dominant antigenic variant among dogs in Taiwan has shifted from CPV-2b to 16]. Similarly, an epidemiological study in Laos and Vietnam demonstrated that over 90% of infected dogs had the CPV-2c variant [17, 18]. CPV-2c was identi ed in various countries in Asia except in Japan [15][16][17][18][19][20]...

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Section: Discussionmentioning

confidence: 72%

mentioning

confidence: 99%

Predominance of canine parvovirus 2b in Japan: an epidemiological study during 2014-2019

Takano

Hamaguchi²,

Hasegawa³

et al. 2021

Arch Virol

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“…In the 2000s, this variant was found with high frequency in Europe and South America, but was relatively rare in Asia, where CPV‐2a was predominant (Decaro & Buonavoglia, ). However, the distribution of CPV‐2c has since expanded, and it has recently been found in Asia including China (Geng et al, ; Wang et al, ; Zhao et al, ), Taiwan (Chiang, Wu, Chiou, Chang, & Lin, ; Lin et al, ), Vietnam (Hoang et al, ), Laos (Vannamahaxay et al, ), Mongolia (Temuujin et al, ) and Thailand (Charoenkul et al, ).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of canine parvovirus type 2c from domestic dogs in Korea

Moon

Jang

Kim

et al. 2020

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV-2) is a viral agent that causes acute haemorrhagic enteritis and, less frequently, fatal myocarditis in domestic and wild carnivores. Non-immunized puppies are particularly susceptible, and the virus causes severe enteritis accompanied with loss of appetite, depression, high fever, vomiting, rapid dehydration and bloody diarrhoea, as well as a wide range of cardiac clinical signs ranging from tachypnea or dyspnoea with exercise to respiratory distress, and resulting in acute heart failure (Appel, Cooper, Greisen,

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“…Changes in the nucleotide sequence were found to occur at a rate of 1-10 -4 to 4-10 -4 changes/nt/year [4] . Although commercial vaccines currently available are mostly based on CPV 2 or CPV 2b, and it is claimed that they can cross-protect against all antigenically distinct types, but the CPV infections are still at large which suggest regular monitoring as an important tool to identify the types of CPV variants related to the infection [9] . Keeping the above points in view, the present study was conducted to detect the variant of Carnivore protoparvovirus 1 present in Mizoram and molecular characterization of the detected variant based on partial VP2 gene.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of 2c variant of Carnivore protoparvovirus1 from Mizoram, India

Akter¹,

Roychoudhury²,

Dutta³

et al. 2020

J. Entomol. Zool. Stud.

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Canine parvovirus2 (CPV2) is associated with acute fatal hemorrhagic diarrhea in pups. The present study was conducted to detect the variant of Carnivore protoparvovirus 1from a case of severe hemorrhagic gastroenteritis in a pup by polymerase chain reaction (PCR) combining with partial VP2 gene sequencing. PCR was performed, targeting a partial segment of the VP2 protein gene, which gives an amplicon of 536 bp. PCR amplicon was cloned and sequenced. Phylogenetic analysis was performed by comparing sequences with all other variants of CPV2 viruses, revealed that the virus belonged to the CPV2c variant (MN218607). The partial VP2 gene sequence revealed a 99.4 to 99.6% sequence similarity with other reported CPV2c variant. Typical substitution of Glutamine (E) instead of Asparagine (N) in the amino acid position 426 could be observed, further confirming the CPV2c variant. Till now, there is no published report available that show the presence of CPV 2c variant in the dog population of Mizoram.

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The first isolation and identification of canine parvovirus (CPV) type 2c variants during 2016–2018 genetic surveillance of dogs in Mongolia

Cited by 15 publications

References 33 publications

Predominance of canine parvovirus 2b in Japan: an epidemiological study during 2014-2019

Predominance of canine parvovirus 2b in Japan: an epidemiological study during 2014-2019

Genetic characterization of canine parvovirus type 2c from domestic dogs in Korea

Molecular detection of 2c variant of Carnivore protoparvovirus1 from Mizoram, India

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