The first experimental research on the pathogenicity of Arcobacter butzleri in zebrafish

Açık, Mehmet Nuri; Yüksel, Hayati; Ulucan, Aykut; Çetinkaya, Burhan

doi:10.1016/j.vetmic.2016.04.016

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…The in vitro interactions of Arcobacter isolates on human colon cell line HT-29/B6 were demonstrated by Karadas et al (2016). Açik et al (2016) showed that A. butzleri induced enteritis, congestion, and necrosis in the liver, kidney, and spleen in zebrafish. Gölz et al (2016) showed that infected gnotobiotic mice had an increased secretion of pro-inflammatory cytokines in small and large intestine and serum samples.…”

Section: Resultsmentioning

confidence: 99%

Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

Oliveira

Pressinotti²,

Carvalho³

et al. 2017

Trop Anim Health Prod

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The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans.

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Section: Resultsmentioning

confidence: 99%

Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

Oliveira

Pressinotti²,

Carvalho³

et al. 2017

Trop Anim Health Prod

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“…Similarly, experimental infection of adult rats with Arcobacter led to watery diarrhea and imbalanced serum electrolytes which depends on the bacterial load, while neonatal albino rats suffered from self-limiting diarrhea, small intestinal and hepatic necrosis (Adesiji 2010;Adesiji et al 2012). Açik et al (2016) for the first time experimentally studied A. butzleri infection in wild zebrafish. The zebrafish were inoculated with mean infective dose (ID 50 ) of A. butzleri as 1.3 £ 10 8 CFU/mL by immersion and 1 £ 10 5 CFU/mL by intraperitoneal injection.…”

Section: Disease In Animals Caused By Arcobactersmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

135

130

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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“…The genus Arcobacter sp. is usually associated with water from sewage [80] or farm e uents such as piggeries [81] but can be common in marine invertebrates such as crabs and oysters [82] and includes some intestinal pathogens of both humans and sh [83,84]. In our study, it was particularly abundant in sh fed the 'control' diets without any supplementation ( ve times more abundant).…”

Section: Discussionmentioning

confidence: 57%

Influence of Seaweed Supplements on the Intestinal Bacteria in the Rabbitfish Siganus Fuscescens: Evidence for a Core Microbiome

Thépot

Slinger

Rimmer

et al. 2020

Preprint

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BackgroundWe know very little about natural variation in microbiomes of marine herbivorous fish in the wild or in captivity (aquaculture). Understanding how the consumption of seaweed influences intestinal microbial communities will shed light on how such phytobiotics could enhance the health and productivity of farmed fish. Here we screened the effects of supplementing the diets of mottled rabbitfish (Siganus fuscescens), a candidate species for international aquaculture development, with 15 different species of seaweeds and functional supplements currently used in aquaculture, on the bacterial communities that colonised their hindguts. ResultsRemarkably, the second most abundant phylum and the majority (53%) of the bacterial genera were not assigned, highlighting a significant knowledge gap for the field of animal microbiomes. Dietary supplementation increased alpha diversity by up to 23% relative to the control fish. Furthermore, most supplements significantly increased the relative abundance of Firmicutes, with similar trends for Proteobacteria and consistent decreases in Bacteroides. Seaweed supplementation also had important effects at the genus level, including significant increases in Fusobacterium sp. in fish fed seaweed - especially the green Caulerpa taxifolia – and overall trends for reduced levels of Arcobacter sp., a genus that includes fish and human pathogens. When we compared microbiomes in our fish to those from two recently published studies of conspecific populations sampled many thousands of kilometres away, the populations were clearly distinct, however there were 55 ASVs that were shared across the three fish populations, of which 35 were present in 50% of all fish sampled.ConclusionThe identification of a core microbiome suggests that a host organism relies on certain microbes for key functions and our findings suggest that this candidate aquaculture species has a core microbiome in its hindgut which is robust to dietary manipulations and broad geographical and temporal variation. This insight will help guide future work investigating the functional and mechanistic bases of these relationships, as will improvements in microbial taxonomic resolution. Supplementation with seaweeds did have subtle influences on bacteria in the hindgut of Siganus fuscescens which could have important impacts on fish health and should be considered as aquaculture systems are developed for this species.

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The first experimental research on the pathogenicity of Arcobacter butzleri in zebrafish

Cited by 10 publications

References 28 publications

Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Influence of Seaweed Supplements on the Intestinal Bacteria in the Rabbitfish Siganus Fuscescens: Evidence for a Core Microbiome

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