The First Epidemic of Dengue Hemorrhagic Fever in French Guiana

Reynes, J.; Laurent, Angela M.; Denis, Vincent; Telliam, E.; Moreau, Jean‐Paul

doi:10.4269/ajtmh.1994.51.545

Cited by 44 publications

(34 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All sera obtained during this study were used for virus isolation in tissue culture as described by Reynes et al (15). Sera were diluted 1:10 in tissue culture medium (Leibovitz; Sigma) and applied to a confluent monolayer of AP61 cells.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Use of Capillary Blood Samples as a New Approach for Diagnosis of Dengue Virus Infection

et al. 2007

View full text Add to dashboard Cite

We evaluated the use of capillary blood samples stored on filter papers for diagnosis of dengue virus infection. Venous and capillary blood samples were collected from 130 patients suspected of having dengue fever. We compared the performances of standard reference methods using capillary blood samples absorbed onto filter papers versus venous blood samples. The resulting sensitivity, specificity, and positive predictive value of tests performed on filter paper compared to those performed on venous blood samples were 81.6% (62/76; 95% confidence interval [CI], 74.9% to 88.3%), 90.7% (49/54; 95% CI, 85.7% to 95.7%), and 92.5% (62/67; 95% CI, 86.2% to 98.8%), respectively. During the acute phase of dengue virus infection (day 1 to day 4), the tests performed on capillary blood samples had a sensitivity of 88.5% (95% CI, 82.0% to 95.0%) and a specificity of 93.8% (95% CI, 88.9% to 98.7%). During the convalescent phase of infection, this method allowed the viral serotype to be determined for 4 of 15 (27%) dengue virus-infected patients for whom virological diagnosis using venous samples was negative. Capillary blood samples could therefore be a good alternative for the diagnosis of dengue virus infection in tropical areas. Indeed, these samples are convenient for storage and transport without the need for a cold chain and simplify the collection of samples from children. Moreover, our results suggest that viral particles persist longer in capillary blood than in peripheral blood. Analysis of the viability of viral particles under these conditions may give new insights into the physiopathology of dengue virus infection and the transmission of dengue virus during outbreaks.

show abstract

Section: Methodsmentioning

confidence: 99%

“…A SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen Life Technologies) was used for first-strand cDNA synthesis according to the manufacturer's recommendations. The initial RT-PCR and subsequent seminested PCR were carried out as previously described (15).…”

Section: Methodsmentioning

confidence: 99%

Use of Capillary Blood Samples as a New Approach for Diagnosis of Dengue Virus Infection

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Isolation was performed on AP 61 and Vero E6 cells with admission serum (diluted 1:10) and CSF (diluted 1:2) as previously described. 4 Dengue virus (DENV) type-specific, JEV, and flavivirus monoclonal antibodies, and Chikungunya virus (CHIKV) and Langat virus (LGTV) immune ascitic fluids were used for identification.…”

Section: Methodsmentioning

confidence: 99%

Etiology of encephalitis syndrome among hospitalized children and adults in Takeo, Cambodia, 1999-2000.

Srey¹,

Sadones²,

Ong³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…The annual number of P. falciparum and P. vivax malaria cases ranges from 3,500 to 4,500. In addition, all 4 dengue virus serotypes have been isolated in the country (7). To determine the frequency of concurrent infection with dengue and malaria in French Guiana, we conducted a 1-year study of patients evaluated in the emergency department of Cayenne Hospital.…”

mentioning

confidence: 99%