The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA

Salaba, O.; Rylková, Kateřina; Vadlejch, Jaroslav; Petrtýl, Miloslav; Scháňková, Š.; Brožová, A.; Jankovská, Ivana; Jebavý, L; Langrová, Iva

doi:10.1007/s00436-012-3215-0

Cited by 18 publications

(12 citation statements)

References 21 publications

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“…According to scientific reports, trichurosis is very common in wild and domestic ruminants. The infection is recorded world-wide, with rates varying in different countries (Salaba et al, 2013;Yaro et al, 2015;Eichenberger et al, 2018). In our opinion, domination of one species over several other Trichuris species in a host population is to some degree dependent on the nematode specifics of embryogenesis which occurs outside the host.…”

Section: Discussionmentioning

confidence: 79%

“…In domestic ruminants (cattle, sheep, goats), the most common species of this genus are T. skrjabini (Baskakov, 1924), Т. оvis (Abildgaard, 1795) and Т. globulosa (Linstow, 1901). For example, in Europe the Тrichuris infection rates in sheep range from 41.8% to 100% (Salaba et al, 2013). In South-Western Turkey, T. skrjabini pathogens were found in 74% of examined sheep, Т. оvis was recorded in 72% of studied animals (Umur & Yukari, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)

Мельничук

Berezovsky

2018

Biosys. divers.

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Biological specifics of Trichuris оvis Abildgaard, 1795 and T. globulosa Linstow, 1901 parasitizing domestic sheep were analyzed enhancing the species identification of these nematode species. The embryonic development of nematodes was timed, survival of embryonic stages was determined taking into account their morphological and metric specifics in laboratory culture. Trichuris eggs were isolated from gonads of adult female nematodes collected from caeca of dissected sheep. Then the eggs were cultured at optimal temperature to the formation of eggs with mobile larvae. Six morphologically distinct stages of embryogenesis were established in T. оvis and T. globulosa nematodes. The protoplast stage lasted from the 1st to the 12th day in T. оvis and to the 18th day in T. globulosa. Blastomeric formation occurred from the 3rd to the 18th day in T. оvis and from the 3rd to the 21st day in T. globulosa. Bean-shaped embryos formed from the 6th to the 21st day in T. оvis, and from the 9th to the 30th day in T. globulosa. Tadpole-like embryos developed from the 12th to the 24th day in T. оvis and from the 18th to the 33rd day in T. globulosa. Larvae formed in eggs of T. оvis from the 18th to the 27th day, and in eggs of T. globulosa from the 21st to the 36th day. Mobile larvae formed from the 21st to the 30th day in T. оvis, and from the 30th to the 39th day in T. globulosa. At 27 °С, mature eggs with mobile larvae developed in 30 days in T. оvis and in 39 in T. globulosa. The egg survival in laboratory culture was 84.3 ± 4.2 % and 76.3 ± 1.5%, respectively. Developmental changes of metric parameters in Trichuris nematode eggs (length and width of eggs, plug length, eggshell thickness) were species-specific.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)

Мельничук

Berezovsky

2018

Biosys. divers.

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“…This is necessary because many species, such as T. arvicolae and T. muris and females of the species T. ovis and Trichuris discolor, are morphologically indistinguishable (Feliu et al 2000;Salaba et al 2013). Conversely, Cutillas Oliveros et al (2000) could find no differences between sequences of T. ovis rDNA and Trichuris globulosa rDNA (internal transcribed spacers ITS1-5.8S-ITS2).…”

Section: Discussionmentioning

confidence: 99%

Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic

et al. 2015

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Trichuris sp. individuals were collected from Myocastor coypus from fancy breeder farms in the Czech Republic. Using morphological and biometrical methods, 30 female and 30 male nematodes were identified as Trichuris myocastoris. This paper presents the first molecular description of this species. The ribosomal DNA (rDNA) region, consisting of internal transcribed spacer (ITS)-1, 5.8 gene and ITS-2, was sequenced. Based on an analysis of 651 bp, T. myocastoris was found to be different from any other Trichuris species for which published sequencing of the ITS region is available. The phylogenetic relationships were estimated using the maximum parsimony methods and Bayesian analyses. T. myocastoris was found to be significantly closely related to Trichuris of rodents than those of ruminants.

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“…In fact, several cryptic species ( Callejón et al, 2012 ), synonymies ( Oliveros et al, 2000 ), or new species ( Cutillas et al, 2014 ; Callejón et al, 2017 ) have been defined in Trichuris . The more discriminating molecular methods have shown that not all species previously described by morphometrics will remain truly defined species ( Salaba et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Trichuris species eggs from non-human primates by geometric morphometric analysis

García‐Sánchez

Reguera-Gómez

Valero

et al. 2020

International Journal for Parasitology: Parasites and Wildlife

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Human trichuriasis is a neglected tropical disease which affects millions of people worldwide, mostly living in low socio-economic conditions. Numerous studies have been conducted over the past 10 years to compare the different techniques for T. trichiura eggs detection. Our study provides the first geometric morphometric analysis for the specific detection of eggs of Trichuris sp. isolated from stools of macaque ( M. sylvanus) , colobus ( C. g. kikuyensis) , grivets ( C. aethiops) and the Brazza's monkey ( C. neglectus ) from zoos in Spain. Principal Component Analysis (PCA) arises as an efficient method to determine Trichuris spp. eggs. The selected measurements to be included in the PCA were proposed for the first time in the present work, as far as we know, as we could not find previous studies reporting standardized parameters.

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The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA

Cited by 18 publications

References 21 publications

Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)

Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)

Genetic and morphological characterization of Trichuris myocastoris found in Myocastor coypus in the Czech Republic

Differentiation of Trichuris species eggs from non-human primates by geometric morphometric analysis

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