Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)

Abstract

Biological specifics of Trichuris оvis Abildgaard, 1795 and T. globulosa Linstow, 1901 parasitizing domestic sheep were analyzed enhancing the species identification of these nematode species. The embryonic development of nematodes was timed, survival of embryonic stages was determined taking into account their morphological and metric specifics in laboratory culture. Trichuris eggs were isolated from gonads of adult female nematodes collected from caeca of dissected sheep. Then the eggs were cultured at optimal temperature to the formation of eggs with mobile larvae. Six morphologically distinct stages of embryogenesis were established in T. оvis and T. globulosa nematodes. The protoplast stage lasted from the 1st to the 12th day in T. оvis and to the 18th day in T. globulosa. Blastomeric formation occurred from the 3rd to the 18th day in T. оvis and from the 3rd to the 21st day in T. globulosa. Bean-shaped embryos formed from the 6th to the 21st day in T. оvis, and from the 9th to the 30th day in T. globulosa. Tadpole-like embryos developed from the 12th to the 24th day in T. оvis and from the 18th to the 33rd day in T. globulosa. Larvae formed in eggs of T. оvis from the 18th to the 27th day, and in eggs of T. globulosa from the 21st to the 36th day. Mobile larvae formed from the 21st to the 30th day in T. оvis, and from the 30th to the 39th day in T. globulosa. At 27 °С, mature eggs with mobile larvae developed in 30 days in T. оvis and in 39 in T. globulosa. The egg survival in laboratory culture was 84.3 ± 4.2 % and 76.3 ± 1.5%, respectively. Developmental changes of metric parameters in Trichuris nematode eggs (length and width of eggs, plug length, eggshell thickness) were species-specific.

Morphobiological analysis of Trichuris vulpis (Nematoda, Trichuridae), obtained from domestic dogs

“…Biology of Trichuris nematodes is a popular object of study. However, the embryogenesis is studied in the following species: T. ovis, T. skrjabini, T. globulosa (Thapar & Singh, 1954;Melnychuk & Berezovsky, 2018), Trichuris suis (Yevstafieva et al, 2015). The stages and periods of development in those species are significantly different from our data for T. vulpis.…”

“…the maturation of egg to the infectious stage in the environment (Fahmy et al, 1954;Lee, 2002;Stroehlein et al, 2017). There are reports of embryogenesis of Trichuris nematodes, according to which the features distinguishing egg development stages, egg viability and metric parameters are speciesspecific and can be used in species identification (Fataliev, 3013;Yevstafieva et al, 2015;Melnychuk & Berezovsky, 2018). Thus, a comprehensive study of morphobiological specifics of Т. vulpis parasitizing domestic dogs is not only of interest in itself but is of significant importance for the diagnostics of that zoonosis.…”

Effect of temperature on embryogenesis of Trichuris ovis during in vitro cultivation

“…Тому було проведено вивчення впливу температури на ембріональний розвиток трихурисів овець виду T. ovis, які, згідно з науковими дослідженнями, є доволі поширеним видом на території багатьох країн світу (Yaro et al, 2015;Yevstafieva et al, 2018;Melnychuk et al, 2020). (Yevstafieva et al, 2015;Melnychuk & Berezovsky, 2018;Yevstafieva et al, 2019;Yevstafieva et al, 2020).…”

Дослідження Епізоотичної Ситуації Щодо Трихурозу Собак На Території України

“…З-поміж інвазійних хвороб собак у світі значне місце займають хвороби, викликані нематодами травного каналу, серед яких варто відмітити й трихурозну інвазію. Трихуратози тварин наразі викликають надзвичайну зацікавленість науковців та активно вивчаються в різних країнах світу [14][15][16][17][18], у тому числі й дослідниками з Україні [19][20][21][22][23][24][25][26][27][28][29][30], оскільки вражають значне коло господарів, починаючі від гризунів [15] і, закінчуючи людиною [31][32][33]. У собак хворобу викликає нематода виду Trichuris vulpis (Frölich, 1789).…”