The first case of bloodstream infection by Candida intermedia in Japan: the importance of molecular identification

Hasejima, Nobuchika; Kamei, Katsuhiko; Matsubayashi, Minako; Kawabe, Rie; Shimura, Chie; Hijikata, Naoya; Oda, Toshimi; Matsushima, Hidekazu

doi:10.1007/s10156-011-0215-4

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…There is evidence that colony color switching on CHROMagar medium may occur during the course of therapy with amphotericin B; therefore, the presence of colony variants on the chromogenic medium should be investigated as a signal for the emergence of amphotericin B resistance in C. lusitaniae (20). Three cases of C. intermedia were reported in two separated studies in catheter-related candidemia (21,22). In our study, C. intermedia was isolated from three blood samples of a threeyear-old girl with two episodes of candidemia, who was implicated with cerebral palsy and respiratory disorders hospitalized in the PICU for about three months.…”

Section: Discussionmentioning

confidence: 99%

Frequency of Uncommon Clinical Yeast Species Confirmed by ITS-Sequencing

Ruosta

Charsizadeh

Ghahri

et al. 2019

Arch Clin Infect Dis

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Background: Accurate speciation of the clinical yeast isolates is essential to a targeted treatment. Objectives: The aim of this study was to identify and determine the frequency of uncommon and rare yeast species causing fungal infections that may be misidentified. Methods: During a 10-month period, yeast isolates collected from patients referring to or hospitalized in some educational hospitals in Tehran, Iran, were included in this study. In addition to conventional methods such as direct microscopy and culture on CHROMagar Candida, molecular methods including PCR-RFLP and ITS-sequencing were used for the accurate identification of the yeast strains. Results: Among 930 yeast isolates recovered from normally sterile and other clinical specimens, a total number of 27 strains were identified as uncommon Candida species and three were identified as rare non-Candida species yeasts. They included C. kefyr (n = 12), C. lusitaniae (n = 8), C. intermedia (n = 3), C. orthopsilosis, C. guilliermondii, and Trichosporon asahii (each n = 2), and Magnusiomyces capitatus (n = 1). Conclusions: The isolation of less common or rare yeast species, which can cause a variety of infections from superficial to systemic infections, is increasingly reported. Since this uncommon yeast species may exhibit low susceptibility to some antifungal agents, the use of reliable methods for accurate screening of these yeasts is necessary.

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Section: Discussionmentioning

confidence: 99%

Frequency of Uncommon Clinical Yeast Species Confirmed by ITS-Sequencing

Ruosta

Charsizadeh

Ghahri

et al. 2019

Arch Clin Infect Dis

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“…Even though Candida albicans is the mostly incriminated yeast in Candida infections, according to recent studies, the incidence of other non-albicans species is rapidly rising. Among them, C. tropicalis, C. glabrata, C. parapsilosis, C. krusei (now Pichia kudriavzevii), and C. dubliniensis are considered as frequent opportunistic human pathogens [2,[6][7][8][9][10][11]. The prolonged hospitalisation of immunocompromised patients undergoing advanced medical treatment has led to an increase in the incidence of fungal infections, a large proportion of which is due to nonalbicans Candida species, particularly in patients in haematology, organ-transplant and intensive care units [3,4,[12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

<i>Candida</i> <i>Massiliensis</i> sp. nov. Isolated from A Clinical Sample

Ranque¹,

Boulanouar²,

Militiello³

et al. 2022

Preprint

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The majority of Candida species are known as non-pathogenic yeasts and rarely involved in human diseases. However, recently case reports of human infections caused by non-albicans Candida species have increased, mostly in immunocompromised hosts. Our study aimed to describe and caracterise as thoroughly as possible, a new species of the Candida genus, named here Candida massiliensis (PMML0037), isolated from a clinical sample of human sputum. We compared genetic data based on the sequences of four genetic regions: "Internal Transcribed Spacers" of rRNA, D1/D2 domains (28S large subunit rRNA) and part of the genes encoding Translation Elongation Factor 1-α and β-tubulin2, to morphological characters, from scanning electron microscopy (TM 4000 Plus, SU5000), physiological, including the results of oxidation and assimilation tests of different carbon sources by the Biolog system, and chemical mapping by Energy-Dispersive X-ray Spectroscopy. Lastly, the in vitro antifungal susceptibility profile was performed using the E-testTM exponential gradient method. The multilocus analysis supported the genetic position of Candida massiliensis (PMML0037) as a new species of the genus Candida, and the phenotypic analysis highlighted its unique morphological and chemical profile when compared to other Candida species included in the study.

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