The Fibrinolytic Agents in Saline Extracts of Human Tissues

The fibrinolytic activity of human oral epithelium and epidermis

“…Fibrinolytic activity 683 The fibrin plate experiments showed that plasminogen activators can be extracted from both oral epithelium and epidermis with potassium thiocyanate, the fibrinolytic activity of similar tris buffer extracts being either much reduced or absent. It has been suggested that the stable activator present in thiocyanate extracts of tissue may not be available for normal physiological processes (Albrechtsen, 1958) and this suggestion is supported by the fibrinolytic autograph experiments which showed lysis only over epithelium from which tris buffer extracts also showed fibrinolytic activity with fibrin plates.…”

“…As a result it is often impossible to determine whether or not plasminogen activators arc also present in the epithelium. Two dilferent plasminogen activators can be extracted from connective tissue (Albrechtsen, 1958): a labile one extractable with saline and a stable one more readily extractable with potassium thiocyanate. Nishioka & Ryan {1971) found no fibrinolytic activity in tris buffer extracts of epidermis, but potassium thiocyanate extracts were not investigated.…”

On Fibrinolysis: A. In Pregnancy, Labour, Puerperium and During Treatment with Sex Hormones B. In Human Ontogenesis and in Human Organ Culture

“…The relationship between the stable tissue activator and the labile blood activator is unknown. In some tissues Albrechtsen (1958) observed two types of plasminogen activator, one acid stable resembling that assayed with KSCN extraction and one labile obtained at saline extraction. He thought that the latter might be identical with the labile blood activator.…”

“…The activator is released in a labile form into the blood stream maintaining the spontaneous fibrinolytic activity of the blood. They think that the release of labile plasminogen activator into intercellular spaces may account for the quota of labile activator demonstrated by Albrechtsen (1958) in saline extracts of some tissues. Kucinsky et al (1968) have also suggested the possibility that "plasma activator is probably derived from tissue activator, expecially that found in relation to blood vessels".…”

Studies on thromboplastic and fibrinolytic activities of valvular tissue in rheumatic valvular disease

“…However, as the saline extract showed high activity, we employed the physiological saline extraction method [8] in this investigation. The average of triplicate experiments with each sample was used to express fibrinolytic activity as the diameter in mm 2 of the lyzed zones on fibrin plates after 18 h incubation at 37 ° C. Fibrinolytic activity was localized according to the method of Todd [9].…”