The Fibrinogen Receptor FbsA Promotes Adherence of
            <i>Streptococcus agalactiae</i>
            to Human Epithelial Cells

Schubert, Axel; Zakikhany, Katherina; Pietrocola, Giampiero; Meinke, Andreas; Speziale, Pietro; Eikmanns, Bernhard J.; Reinscheid, Dieter J.

doi:10.1128/iai.72.11.6197-6205.2004

Cited by 87 publications

(84 citation statements)

References 50 publications

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“…This upregulation could be due to increased activity of CovR/ S rather than upregulation of gene expression. Although scpB, lmb, and fbsA play important roles in GBS pathogenesis by aiding in attachment and invasion of host cells, [28][29][30] their importance for survival inside macrophages has yet to be determined. Consequently, future studies should focus on constructing GBS mutants to better understand the role these genes play inside the macrophage.…”

Section: Cps Type Percent Survivalmentioning

confidence: 99%

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

et al. 2016

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Group B Streptococcus (GBS), a leading cause of neonatal sepsis and meningitis, asymptomatically colonizes up to 30% of women and can persistently colonize even after antibiotic treatment. Previous studies have shown that GBS resides inside macrophages, but the mechanism by which it survives remains unknown. Here, we examined the ability of 4 GBS strains to survive inside macrophages and then focused on 2 strains belonging to sequence type (ST)-17 and ST-12, to examine persistence in the presence of antibiotics. A multiple stress medium was also developed using several stressors found in the phagosome to assess the ability of 30 GBS strains to withstand phagosomal stress. The ST-17 strain was more readily phagocytosed and survived intracellularly longer than the ST-12 strain, but the ST-12 strain was tolerant to ampicillin unlike the ST-17 strain. Exposure to sub-inhibitory concentrations of ampicillin and erythromycin increased the level of phagocytosis of the ST-17 strain, but had no effect on the ST-12 strain. In addition, blocking acidification of the phagosome decreased the survival of the ST-17 strain indicating a pHdependent survival mechanism for the ST-17 strain. Congruent with the macrophage experiments, the ST-17 strain had a higher survival rate in the multiple stress medium than the ST-12 strain, and overall, serotype III isolates survived significantly better than other serotypes. These results indicate that diverse GBS strains may use differing mechanisms to persist and that serotype III strains are better able to survive specific stressors inside the phagosome relative to other serotypes.

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Section: Cps Type Percent Survivalmentioning

confidence: 99%

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

et al. 2016

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“…This implies that S. agalactiae uses a different mechanism to adhere to A549 cells. In fact, both pili and the FbsA protein have been demonstrated to mediate binding to A549 cells (Schubert et al, 2004;Dramsi et al, 2006). While transcription of the pilA gene was reduced in the rga mutant, that of fbsA was increased.…”

Section: Discussionmentioning

confidence: 99%

“…Srr-1, PilA and FbsA were previously reported to be involved in the bacterial adherence to host cells (Maisey et al, 2007;Samen et al, 2007;Schubert et al, 2004). In particular, it was previously demonstrated that Srr-1 is involved in the adherence of S. agalactiae to the human larynx cell line HEp-2, and that FbsA mediates the binding to human pulmonary cell line A549 (Samen et al, 2007;Schubert et al, 2004).…”

Section: Role Of Rga In Adherence To Epithelial Cellsmentioning

confidence: 99%

“…In particular, it was previously demonstrated that Srr-1 is involved in the adherence of S. agalactiae to the human larynx cell line HEp-2, and that FbsA mediates the binding to human pulmonary cell line A549 (Samen et al, 2007;Schubert et al, 2004). Therefore, the ability of the three strains NEM316_pAT18, Drga_pAT18 and Drga_pATrga to adhere to these cell lines was investigated.…”

Section: Role Of Rga In Adherence To Epithelial Cellsmentioning

confidence: 99%

“…The FbsA protein has been shown to play a major role in the bacterial binding to human A549 cells, and also to bind human fibrinogen (Schubert et al, 2002(Schubert et al, , 2004. Thus, fibrinogen binding was quantified for the Drga mutant.…”

Section: Deletion Of Rga Influences Bacterial Adherence To Immobilizementioning

confidence: 99%

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Rga is a regulator of adherence and pilus formation in Streptococcus agalactiae

et al. 2011

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Streptococcus agalactiae is the leading cause of bacterial sepsis and meningitis in neonates and is also the causative agent of several serious infections in immunocompromised adults. S. agalactiae encounters multiple niches during an infection, suggesting that regulatory mechanisms control the expression of specific virulence factors in this bacterium. The present study describes the functional characterization of a gene from S. agalactiae, designated rga, which encodes a protein with significant similarity to members of the RofA-like protein (RALP) family of transcriptional regulators. After deletion of the rga gene in the genome of S. agalactiae, the mutant strain exhibited significantly reduced expression of the genes srr-1 and pilA, which encode a serine-rich repeat surface glycoprotein and a pilus protein, respectively, and moderately increased expression of the fbsA gene, which encodes a fibrinogen-binding protein. Electrophoretic mobility shift assays demonstrated specific DNA binding of purified Rga to the promoter regions of pilA and fbsA, suggesting that Rga directly controls pilA and fbsA. Adherence assays revealed significantly reduced binding of the Δrga mutant to epithelial HEp-2 cells and to immobilized human keratin 4, respectively. In contrast, the adherence of the Δrga mutant to A549 cells and its binding to human fibrinogen was significantly increased. Immunoblot and immunoelectron microscopy revealed that the quantity of pilus structures was significantly reduced in the Δrga mutant compared with the parental strain. The wild-type phenotype could be restored by plasmid-mediated expression of rga, demonstrating that the mutant phenotypes resulted from a loss of Rga function.

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Screening of binding activity of Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus suis to berries and juices

Toivanen

Huttunen

Soininen

et al. 2010

Phytotherapy Research

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Antiadhesion therapy is a promising approach to the fight against pathogens. Antibiotic resistance and the lack of effective vaccines have increased the search for new methods to prevent infectious diseases. Previous studies have shown the antiadhesion activity of juice from cultivated cranberries (Vaccinium macrocarpon Ait.) against bacteria, especially E. coli. In this study, the binding of two streptococcal strains, Streptococcus pneumoniae and Streptococcus agalactiae, to molecular size fractions (FI, FII and FIII, <10 kDa, 10-100 kDa, and >100 kDa, respectively) of berries and berry and fruit juices from 12 plant species were studied using a microtiter well assay. For Streptococcus suis a hemagglutination inhibition assay was used. In general, binding activity was detected especially to wild cranberry (Vaccinium oxycoccos L.) and to other Vaccinium species. S. pneumoniae cells bound most to cranberry juice fraction FI and S. agalactiae cells to cranberry fraction FIII. Hemagglutination induced by S. suis was most effectively inhibited by cranberry fraction FII. NMR spectra of some characteristic active and non-active fractions were also measured. They indicate that fractions FII and FIII contained proanthocyanidins and/or other phenolic compounds. The results suggest Vaccinium berries as possible sources of antiadhesives against bacterial infections.

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The Fibrinogen Receptor FbsA Promotes Adherence of Streptococcus agalactiae to Human Epithelial Cells

Cited by 87 publications

References 50 publications

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

Differing mechanisms of surviving phagosomal stress among group BStreptococcusstrains of varying genotypes

Rga is a regulator of adherence and pilus formation in Streptococcus agalactiae

Screening of binding activity of Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus suis to berries and juices

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