Screening of binding activity of Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus suis to berries and juices

Toivanen, Marko; Huttunen, Sanna; Soininen, Pasi; Laatikainen, Reino; Loimaranta, Vuokko; Haataja, Sauli; Finne, Jukka; Lapinjoki, Seppo; Tikkanen-Kaukanen, Carina

doi:10.1002/ptr.2939

Cited by 18 publications

(19 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, several studies have focused on alternative strategies to cure pneumococcal infections 22–24 . It has been known for a long time that plants are a source of antimicrobial compounds 25 .…”

Section: Discussionmentioning

confidence: 99%

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Mitsuwan

Olaya-Abril

Calderón-Santiago

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The emergence of antibiotic-resistant pathogenic bacteria is a healthcare problem worldwide. We evaluated the antimicrobial activity of rhodomyrtone, an acylphloroglucinol present in Rhodomyrtus tomentosa leaves, against the human Gram-positive pathogen Streptococcus pneumoniae. The compound exhibited pronounced anti-pneumococcal activity against a broad collection of clinical isolates. We studied the effects at the molecular level by integrated proteomic and metabolomic analysis. The results revealed alterations in enzymes and metabolites involved in several metabolic pathways including amino acid biosynthesis, nucleic acid biosynthesis, glucid, and lipid metabolism. Notably, the levels of two enzymes (glycosyltransferase and UTP-glucose-1-phosphate uridylyltransferase) and three metabolites (UDP-glucose, UDP-glucuronic acid and UDP-N-acetyl-D-galactosamine) participating in the synthesis of the pneumococcal capsule clearly diminished in the bacterial cells exposed to rhodomyrtone. Rhodomyrtone-treated pneumococci significantly possessed less amount of capsule, as measured by a colorimetric assay and visualized by electron microscopy. These findings reveal the utility of combining proteomic and metabolomic analyses to provide insight into phenotypic features of S. pneumoniae treated with this potential novel antibiotic. This can lead to an alternative antibiotic for the treatment of S. pneumoniae infections, because of the growing concern regarding antimicrobial resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Mitsuwan

Olaya-Abril

Calderón-Santiago

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In previous studies it was shown that N. meningitidis pili , Streptococcus pneumoniae bacterial cells and Streptococcus agalactiae bacteria bind to berry molecular size fractions especially from Vaccinium species and they inhibit hemagglutination caused by Streptococcus suis (Toivanen et al ., 2009, 2010). The results (Toivanen et al ., 2009) showed that berry juice molecular size fractions of 10–100 kDa inhibited the binding of isolated N. meningitidis pili to membrane‐bound epithelial cells in a dot assay.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions

Toivanen

Huttunen

Lapinjoki

et al. 2010

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

The adhesion of pathogens to host tissues is the requirement for the initiation of the majority of infectious diseases. It was shown recently that the binding of Neisseria meningitidis pili to immobilized human epithelial cells is inhibited by molecular size fractions (10–100 kDa) of berry juices. Additionally, the isolated meningococcal pili bound to polyphenolic fractions of berry juices. The present study investigated the antiadhesive effects of berry juice polyphenolics against living meningococcal bacteria in a human epithelial cell culture model. The ability of bilberry, cranberry, crowberry and lingonberry juice polyphenolic fractions to inhibit the attachment of N. meningitidis bacteria to HEC‐1B human epithelial cells in a cell culture model was examined. The antibacterial effect of the fractions was tested using a microtiter broth microdilution assay. The most effective adhesion inhibition of 75% was achieved with cranberry juice polyphenolic fraction followed by crowberry (63%), bilberry (63%) and lingonberry (57%) juice polyphenolic fractions. Bacterial survival rates after incubation with the fractions varied between 75–100%. The present results suggest berry juice polyphenols as inhibitors of adherence of N. meningitidis. Thus the binding of meningococci to berry juice polyphenols might be protective for the host against the infection. Copyright © 2010 John Wiley & Sons, Ltd.

show abstract

“…Recently, it was reported that juice fractions of different molecular size prepared from bilberry ( Vaccinium myrtillus ), blackcurrant ( Ribes nigrum ), cranberry ( Vaccinium oxycoccus ), crowberry ( Empetrum nigrum and hermaphroditum ) and lingonberry ( Vaccinium vitis‐idaea ) exhibit antiadhesion activity against a Gram‐negative human pathogen Neisseria meningitidis (Toivanen et al , 2009). Also binding or inhibitory activities of molecular size fractions prepared from Vaccinium berries and crowberry were shown against different pathogenic streptococcal strains of human or animal origin (Toivanen et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

Antiaggregation potential of berry fractions against pairs of Streptococcus mutans with Fusobacterium nucleatum or Actinomyces naeslundii

Riihinen

Ryynänen

Toivanen

et al. 2010

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

Coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. This is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kDa, FI; 10–100 kDa, FII; >100 kDa, FIII) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: Streptococcus mutans with Fusobacterium nucleatum or Actinomyces naeslundii. Inhibitory and reversal activity was found in the molecular size fractions FII and FIII of bilberry, blackcurrant, crowberry and lingonberry. The active fractions contained higher amounts of polyphenols (5–12% of soluble solids) than those without activity (<2% of soluble solids). Proanthocyanidins dominated in the active lingonberry juice fractions FII and FIII and also small amounts of anthocyanins were detected. Anthocyanins, proanthocyanidins and flavonol glycosides were prevalent in FII and FIII fractions of bilberry, blackcurrant and crowberry juices. Comparable amounts of sugars and titratable acids were present in the latter three berry juice fractions of different size. The results indicate that the high molecular size fractions of lingonberry, bilberry, blackcurrant and crowberry juices have antiaggregation potential on common oral bacteria, the potential being associated with their polyphenolic content. Copyright © 2010 John Wiley & Sons, Ltd.

show abstract

Screening of binding activity of Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus suis to berries and juices

Cited by 18 publications

References 23 publications

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions

Antiaggregation potential of berry fractions against pairs of Streptococcus mutans with Fusobacterium nucleatum or Actinomyces naeslundii

Contact Info

Product

Resources

About