2017
DOI: 10.1016/j.acthis.2017.07.002
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The Feulgen reaction: A brief review and new perspectives

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Cited by 25 publications
(28 citation statements)
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“…Cytological preparations from the conjunctival surface were subjected to the Feulgen reaction, a topochemical method specific for DNA/chromatin. 20,30,31 Acid hydrolysis was performed in 4 M HCl for 40 minutes at 25°C for the generation of deoxyribose in an aldehyde form. 20 The hydrolysis reaction was quenched by rapid treatment with cold 0.1 M HCl.…”
Section: Feulgen Reactionmentioning
confidence: 99%
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“…Cytological preparations from the conjunctival surface were subjected to the Feulgen reaction, a topochemical method specific for DNA/chromatin. 20,30,31 Acid hydrolysis was performed in 4 M HCl for 40 minutes at 25°C for the generation of deoxyribose in an aldehyde form. 20 The hydrolysis reaction was quenched by rapid treatment with cold 0.1 M HCl.…”
Section: Feulgen Reactionmentioning
confidence: 99%
“…[24][25][26][27][28][29] Nuclear size and shape, DNA content (ploidy), and chromatin remodeling can be studied in cytological preparations using video image analysis and the Feulgen reaction. 20,30 Video image analysis in Feulgen-stained preparations is recommended to evaluate defined cell populations. 20 In addition to reporting on supraorganizational and functional parameters of the chromatin fiber (compaction and texture), the method provides information on the geometry of the cell nucleus and allows quantification of DNA ploidy by generating an equivalent cytometric of chromosomal ploidy.…”
Section: Introductionmentioning
confidence: 99%
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