The fertility of ram sperm held for 24h at 5°C prior to cryopreservation

Purdy, Phillip H.; Mocé, E.; Stobart, Robert H.; Murdoch, William J.; Moss, G. E.; Larson, Brent; Ramsey, Shawn; Graham, James K.; Blackburn, Harvey D.

doi:10.1016/j.anireprosci.2009.06.014

Cited by 34 publications

(19 citation statements)

References 20 publications

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“…2002), in most studies, CLC‐treated and control sperm exhibit similar fertility rates (in rainbow trout, rams and stallions; Müller et al. 2008; Purdy and Graham 2004b; Purdy et al. 2010; Spizziri et al.…”

Section: Effect Of Cholesterol‐loaded Cyclodextrins On Sperm Fertilizmentioning

confidence: 93%

Use of Cholesterol in Sperm Cryopreservation: Present Moment and Perspectives to Future

Mocé

Blanch

Tomás

et al. 2010

Reprod Domestic Animals

130

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ContentsSperm cryosurvival rates are not optimal for most species. Therefore, new cryopreservation strategies are needed with the objective of increasing the number of surviving sperm and the quality of those sperm after thawing. Cholesterol plays important roles in many sperm functions, including effects on membrane properties. One of these effects is to stabilize membranes at low temperatures. Thus, species that produce sperm which possess high membrane cholesterol : phospholipid ratios are more resistant to cold shock than sperm with low cholesterol : phospholipid ratios. Therefore, increasing the cholesterol content of sperm membranes may be a strategy that can improve sperm quality after freeze-thawing. In this review, information is presented related to using cyclodextrins pre-loaded with cholesterol for cryopreserving sperm from different species. The topics discussed include both in vitro and in vivo assessments of sperm quality after cryopreservation, as well as how increasing sperm cholesterol content affects other sperm functions.

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Section: Effect Of Cholesterol‐loaded Cyclodextrins On Sperm Fertilizmentioning

confidence: 93%

Use of Cholesterol in Sperm Cryopreservation: Present Moment and Perspectives to Future

Mocé

Blanch

Tomás

et al. 2010

Reprod Domestic Animals

130

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show abstract

“…The conception rate of seven ewes subjected to hand-mated natural breeding confirmed the efficacy of the hormonal protocol to synchronize estrous and ovulation. For LAI, a reduced number of sperm (25 × 10 6 sperm/ewe) were inseminated compared to other reports (Purdy et al, 2010;Alvarez et al, 2012;Richardson et al, 2012;Del Olmo et al, 2013) and the number of motile sperm can directly interfere on the conception rate after LAI (Maxwell, 1986;Eppleston and Maxwell, 1995), which could explain the low conception rate obtained in both groups inseminated. In that regard, our intention was to avoid using a large number of sperm that could potentially mask real differences in sperm quality and function (Eppleston and Maxwell, 1995;Amann and Waberski, 2014) due to compensable sperm defects (Saacke, 2008).…”

Section: Discussionmentioning

confidence: 63%

“…However, it is noteworthy that prolonged pre-freezing equilibration did not consistently improve in vitro or in vivo aspects of sperm quality. In this regard, Purdy et al (2010) reported no improvement with 24 h of pre-freezing equilibration at 5°C on motion parameters or conception rate of ram semen.…”

Section: Discussionmentioning

confidence: 96%

Influence of catalase and pre-freezing equilibration on post-thaw semen quality and conception rate in ewes laparoscopically inseminated

Câmara

Pinto

et al. 2016

Anim Reprod

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The aim of this study was to investigate the effects of catalase and pre-freezing equilibration during ram sperm cryopreservation on motility and membrane and acrosomal integrity of frozen-thawed semen, as well as conception rate following laparoscopic timedinsemination. Semen was collected from four mature Dorper rams, pooled and diluted in Tris egg-yolk extender basic solution (CON), or this solution supplemented with catalase (CAT; 20 U/100 × 10 6 sperm). Extended semen was packaged in 0.25 ml mini straws (25 × 10 6 sperm/straw), chilled (to 5°C), and then either frozen immediately (CON and CAT) or maintained at 5°C for 12 h of pre-freezing equilibration (CON12 and CAT12). Immediately after thawing and at 1 h after incubation at 37°C, kinematic parameters (CASA), plasma membrane integrity (PI-FITC), and acrosomal status (FITC-PNA) of sperm were assessed. There were no significant differences among the four groups on sperm traits evaluated immediately postthaw. However, after 1 h of incubation, total motility (46.7 and 25.0%) and plasma membrane integrity (38.7 and 25.7%) were higher (P < 0.05) in CAT12 than CON. When these two treatments were used for laparoscopic timed artificial insemination of ewes (with synchronized ovulation), conception rate was similar for CAT12 and CON (32.8%, n = 61 vs. 27.3%, n = 55). In conclusion, the combination of catalase and pre-freezing equilibration resulted in significantly improved quality of post-thawed ram semen without affecting conception rate in fixed-time laparoscopically intrauterine inseminated ewes.

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“…Possible reasons for this difference are the slightly lower glycerol concentration and the longer preservation time used in our study, which could benefit the treatments with glycerol. With frozen–thawed semen, other researchers (Purdy 2006; Purdy et al. 2010) did not observe significant differences either in vitro or in vivo after equilibration at 5°C for 48 h before freezing ram semen in Tris–egg yolk–glycerol.…”

Section: Discussionmentioning

confidence: 87%

Conception Rates in Ewes after AI with Ram Semen Preserved in Milk–Egg Yolk Extenders Supplemented with Glycerol

Olivera-Muzante

Fierro

Gil

2010

Reprod Domestic Animals

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This study aimed at comparing the effect of ram semen preserved at 5 °C on two milk-based extenders (UHT skim milk or INRA-96(®) , 5% egg yolk) supplemented with 2% glycerol, and the preservation time (24 and 48 h) on conception rates after cervical AI of ewes. In two field trials, 1198 Merino ewes were cervical AI in spontaneous oestrus. In Experiment 1, pooled semen (6 rams) was extended in UHT-base (fresh, control) or chilled for 24 h in UHT5Y (UHT-base 5% egg yolk), INRA5Y (INRA-96(®) 5% egg yolk), UHT5Y2G (UHT5Y 2% glycerol) or INRA5Y2G (INRA5Y 2% glycerol). In Experiment 2, AI was performed with pooled semen (7 rams) used fresh (extended in UHT-base or UHT5Y2G, control groups) or chilled (extended in UHT5Y2G) for 24 or 48 h. Conception rate was determined by ultrasound 40 days after AI. INRA-96(®) - had similar conception as UHT-preserved semen (56.7 vs 55.4%, p>0.05). Addition of 2% glycerol did not modify the results (56.8 vs 55.2%, p>0.05). Fresh semen extended in UHT-base, and UHT5Y2G yielded similar conception rates (60 vs 64%, p>0.05). Preservation for 24 or 48 h in UHT5Y2G gave similar results (49 vs 47%; p>0.05). In conclusion, ram semen chilled for 24 h in UHT- or INRA-96(®) -based extenders yielded similar results, and glycerol addition did not have a detrimental effect. UHT5Y2G might be used to extend ram semen for fresh AI, or to preserve it for 24 or 48 h with acceptable results.

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The fertility of ram sperm held for 24h at 5°C prior to cryopreservation

Cited by 34 publications

References 20 publications

Use of Cholesterol in Sperm Cryopreservation: Present Moment and Perspectives to Future

Use of Cholesterol in Sperm Cryopreservation: Present Moment and Perspectives to Future

Influence of catalase and pre-freezing equilibration on post-thaw semen quality and conception rate in ewes laparoscopically inseminated

Conception Rates in Ewes after AI with Ram Semen Preserved in Milk–Egg Yolk Extenders Supplemented with Glycerol

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