Conception Rates in Ewes after AI with Ram Semen Preserved in Milk–Egg Yolk Extenders Supplemented with Glycerol

Olivera-Muzante, J.; Fierro, S.; Gil, J.

doi:10.1111/j.1439-0531.2010.01698.x

Cited by 9 publications

(6 citation statements)

References 32 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar finding was observed by other scientists (Kasimanickam et al, 2007;Gundogan et al, 2011). Our study revealed that the motility of spermatozoa was reduced with increasing preservation time, however, the mean motile sperm was 75.5% up to 48 h of storage, a time which is acceptable for AI according to Olivera-Muzante et al (2011). Taken together, these results showed that Triladyl gives better support to sperm motility after storage at 4°C than TFE.…”

Section: Discussionmentioning

confidence: 53%

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Rekha¹,

Zohara²,

Bari³

et al. 2016

Anim Reprod

View full text Add to dashboard Cite

Two different extenders were compared for their effects on preservation of semen from Indigenous rams and on pregnancy rate (PR) in Indigenous ewes. Semen was collected from nine Indigenous rams (Ovis aries) once a week using an artificial vagina. Each ejaculate was divided into 2 aliquots, diluted with either commercial (Triladyl ® ) or locally manufactured (tris, fructose, citric acid, egg yolk: TFE, prepared in own laboratory) extenders and kept at 4°C for 48 h. Motility, viability, functional integrity and morphological changes were evaluated at 0, 24 and 48 h. Synchronized oestrus ewes inseminated transcervically with 24 and 48 h of preserved chilled semen diluted with Triladyl and TFE extenders separately. Semen preserved in Triladyl had better motility, viability, and functional integrity at 24 and 48 h (P < 0.001) than did in TFE. The morphologically normal spermatozoa up to 48 h of preservation did not differ between extenders. However, in abnormalities studied, Triladyl had detrimental effect on sperm acrosome and TFE on sperm tail (P < 0.001) at 24 and 48 h of preservation. But, midpiece was not affected by any extender (P > 0.05) over the entire period of preservation. The quality of semen decreased (P < 0.001) with increasing preservation time for both extenders. The extenders did not differ (P > 0.05) the overall PR after transcervical AI (TCAI) in ewes. Increased preservation time (48 h) negatively affected the PR in TFE extended semen compared with than that of Triladyl. The results suggest that the quality of chilled semen (motility, viability, and functional integrity) is more improved when preserved in Triladyl than if extended with a TFE. PR may higher when TCAI is performed with chilled semen preserved in Triladyl for a longer time than TFE. However, TFE extender may be used to dilute the semen for chilling and used in TCAI to get similar PR of Triladyl up to 24 h of preservation.

show abstract

Section: Discussionmentioning

confidence: 53%

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Rekha¹,

Zohara²,

Bari³

et al. 2016

Anim Reprod

View full text Add to dashboard Cite

show abstract

“…Considerable progress in semen preservation has been made over the past few decades; however, the decline that occurs in sperm quality during this process has not been resolved [5,11,20]. During in vitro preservation, there is a risk of oxidative damage to sperm owing to ROS accumulation [8,12].…”

Section: Discussionmentioning

confidence: 99%

“…The mammalian sperm membrane contains a large amount of polyunsaturated fatty acids (PUFA). When ROS is excessive, the PUFA of the sperm membrane is attacked, which causes lipid peroxidation (LPO) [10,20]. LPO of sperm membranes can also lead to failure of the acrosome reaction [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…When ROS is excessive, the PUFA of the sperm membrane is attacked, which causes lipid peroxidation (LPO) [10,20]. LPO of sperm membranes can also lead to failure of the acrosome reaction [20,21]. Excessive ROS damages cellular proteins, and the lipid structure of the cell membrane, furthermore excessive ROS causes gene mutations and accelerates cell death [10].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 °C

Wen

Feng

et al. 2019

Animals

View full text Add to dashboard Cite

Simple SummaryArtificial insemination (AI) is widely used in goats, stimulating the development of semen preservation techniques. Oxidative stress is considered to be the main cause of sperm quality decline over time. In this study, we explored the effect of grape seed procyanidin extract (GSPE) during the liquid preservation of goat semen. The results showed that adding GSPE into the basic diluent enhanced sperm quality by eliminating oxidative stress. The most suitable concentration for the preservation of goat semen at 4 °C was 30 mg/L. This work suggests that promotes the viability of goat semen stored at low temperatures and provides a theoretical foundation for the development of more efficient diluents.AbstractGrape seed procyanidin extract (GSPE) has been shown to possess antioxidative effects. This experiment was designed to study the effect of GSPE during the liquid storage of goat semen. Semen samples were collected from six sexually mature goats. The samples were treated with different concentrations of GSPE (10, 30, 50, and 70 mg/L) in basic diluent and stored at 4 °C for 120 h; samples without GSPE were used as the control group. The results showed that sperm motility, acrosome membrane integrity, mitochondrial activity, plasma membrane integrity, total antioxidative capacity (T-AOC), catalase (CAT) activity, and superoxide dismutase (SOD) activity in the treatment groups were significantly higher than in the control group, whereas malondialdehyde (MDA) content was lower than in the control group (p < 0.05). In the treatment group, sperm quality in the 30 mg/L GSPE group was significantly higher than the other groups (p < 0.05). Furthermore, artificial insemination (AI) results showed that litter sizes were higher in the 30 mg/L GSPE group than in the control group (p < 0.05). In summary, this experiment showed that adding GSPE to the basic diluent improved sperm quality and that 30 mg/L of GSPE was the most suitable concentration for the liquid preservation of goat semen at 4 °C.

show abstract

“…Paulenz et al (2003) reported a 51.5 and 61.9% lambing rate using semen stored at 5°C diluted in a commercial TRIS-based or milk extender, respectively, in Norwegian Crossbred ewes. The overall conception rate of ewes inseminated with diluted (UHTbase) chilled semen (after 24 h of preservation at 5°C) was significantly lower than the control group inseminated with diluted fresh semen after 1 h of preservation at room temperature (56% vs 74% respectively, P<0.05) (Olivera-Muzante et al 2011).…”

Section: Resultsmentioning

confidence: 81%

Effect of extender type and cold storage of fresh semen on reproductive indices of Karakul ewe following fixed time artificial insemination

Hashemi¹,

Safdarian

HASHEMI

2018

Indian J of Anim Sci

View full text Add to dashboard Cite

Semen quantity and quality are affected by transportation and frequent semen collection in ram when they are transported from nucleus flock to target farms for in situ artificial insemination (AI) of ewes with fresh semen. The current study was designed to introduce the best method of fresh semen preservation for fixed time AI in Karakul sheep. The adult Karakul ewes were allocated to 6 groups (30 ewes in each group) during the breeding season and each group was inseminated with one of the following treatments, viz. fresh semen without extender, fresh semen diluted with Tris-egg yolk or milk, cooled semen (stored at 10°C for 8 h) without extender, and cooled semen diluted with Tris-egg yolk or milk. Lambing and fecundity rates were the highest when ewe was inseminated with undiluted fresh semen (71.42 and 85.71%, respectively). These reproductive indices in ewes, which were inseminated with diluted fresh semen or undiluted cooled semen were significantly lower than those recorded in inseminated ewes with undiluted fresh semen. The type of extender and storage of diluted semen at 10°C for 8 h did not significantly affect lambing rate of Karakul ewe. Results suggested that use of undiluted fresh semen and diluted fresh semen in milk or Tris-egg yolk extenders are preferable for fixed time AI in ewes of genetic improvement center and Karakul sheep flocks located near to this center, respectively.

show abstract

Conception Rates in Ewes after AI with Ram Semen Preserved in Milk–Egg Yolk Extenders Supplemented with Glycerol

Cited by 9 publications

References 32 publications

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep

Grape Seed Procyanidin Extract (GSPE) Improves Goat Sperm Quality When Preserved at 4 °C

Effect of extender type and cold storage of fresh semen on reproductive indices of Karakul ewe following fixed time artificial insemination

Contact Info

Product

Resources

About