The ferrous iron oxidation kinetics of Thiobacillus ferrooxidans in batch cultures

Boon, Mieke; Ras, Cor; Heijnen, Joseph J.

doi:10.1007/s002530051467

Cited by 32 publications

(25 citation statements)

References 8 publications

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“…It has been shown that a Michaelis-Menten type rate of equation describes the specific rate of Fe 2+ utilization of A. ferrooxidans and L. ferrooxidans bacteria in continuous cultures by incorporating ferric ion inhibition, and neglecting the relatively low threshold value of ferrous iron [3,4,15]:…”

Section: Model Reactionsmentioning

confidence: 99%

Model-based evaluation of ferrous iron oxidation by acidophilic bacteria in chemostat and biofilm airlift reactors

Ebrahimi

Faraghi

Hosseini

2015

Journal of Industrial Microbiology and Biotechnology

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This article presents a model-based evaluation of ferrous iron oxidation in chemostat and biofilm airlift reactors inoculated with a mixed culture of Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans bacteria. The competition between the two types of bacteria in the chemostat and in the biofilm airlift reactors together with the distribution of both bacteria along the biofilm thickness at different time sections has been studied. The bacterial distribution profiles along the biofilm in the airlift reactor at different time scales show that in the beginning A. ferrooxidans bacteria are dominant, but when the reactor operates for a long time the desirable L. ferrooxidans species outcompete A. ferrooxidans as a result of the low Fe(2+) and high Fe(3+) concentrations. The results obtained from the simulation were compared with the experimental data of continuously operated internal loop airlift biofilm reactor. The model results are in good agreement with the experimental results.

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Section: Model Reactionsmentioning

confidence: 99%

Model-based evaluation of ferrous iron oxidation by acidophilic bacteria in chemostat and biofilm airlift reactors

Ebrahimi

Faraghi

Hosseini

2015

Journal of Industrial Microbiology and Biotechnology

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“…For batch experiments, cells (in suspension) were added to a basal salt solution comprising FeSO 4 and Fe 2 (SO 4 ) 3 salts to yield a 310-ml solution with known Fe(II), Fe(III), and cell concentrations. Oxygen was supplied in excess by bubbling air through the medium.…”

Section: Preparation Ofmentioning

confidence: 99%

“…A technique for estimating kinetics constants from the growth kinetics of batch cultures was employed by Liu et al (23), Gomez et al (12), and Boon et al (4). In this technique, cells harvested during the logarithmic growth phase are introduced into a fresh medium with a known initial Fe(II) concentration.…”

mentioning

confidence: 99%

“…Moreover, those authors found that the interference of the Fe(III) concentration in the o-phenanthroline analysis is due to direct color formation caused by the reaction of Fe(III) with o-phenanthroline and that the nature of the response of o-phenanthroline to Fe(III) is nonlinear. Therefore, correcting the measurements by comparing the absorbance to a blank to which no o-phenanthroline has been added, as was done, for example, by Boon et al (4), is clearly incorrect. In another study, Nyavor et al (34) did not report at all on the Fe(II) analysis method that was used, a fact which tends to detract from the reliability of the results.…”

mentioning

confidence: 99%

“…However, the methodology presented is general and can be applied regardless of the "culture history". 4 . Cell suspensions used in the experiments were taken from the flask and filtered during the logarithmic phase of growth by using a Whatman no.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Improved Experimental and Computational Methodology for Determining the Kinetic Equation and the Extant Kinetic Constants of Fe(II) Oxidation byAcidithiobacillus ferrooxidans

Molchanov

Gendel

Ioslvich

et al. 2007

Appl Environ Microbiol

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Comparison of biooxidation with carbon dioxide assimilation during bacterial growth on ferrous ion or elemental sulfur

Lizama

Zieliński²,

Kerby³

et al. 2001

Biotech & Bioengineering

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Biomass and oxygen uptake activity profiles of a mixed bioleaching culture were studied and compared at various temperatures. Bacteria were grown on ferrous ion or elemental sulfur in a Micro-Oxymax respirometer apparatus that allowed measurement of both oxygen consumption and carbon dioxide assimilation. Balanced growth was observed between 10 degrees C and 35 degrees C, with an optimum at 30 degrees C, on both energy sources. No significant growth was observed at the lowest temperature used, 5 degrees C, or at the highest temperature used, 40 degrees C. The oxygen to carbon dioxide molar yield was 50:1 when growing on ferrous ion but only 17:1 when growing on elemental sulfur. Upon transfer from a sulfide ore to a new energy source, greater numbers in the inoculum reduced the duration of the lag phase. Lag phase duration was also reduced by proximity to the optimum growth temperature. A longer lag phase decreased the achievable growth rate of the cells exponentially, significantly affecting biooxidation activity.

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The ferrous iron oxidation kinetics of Thiobacillus ferrooxidans in batch cultures

Cited by 32 publications

References 8 publications

Model-based evaluation of ferrous iron oxidation by acidophilic bacteria in chemostat and biofilm airlift reactors

Model-based evaluation of ferrous iron oxidation by acidophilic bacteria in chemostat and biofilm airlift reactors

Improved Experimental and Computational Methodology for Determining the Kinetic Equation and the Extant Kinetic Constants of Fe(II) Oxidation byAcidithiobacillus ferrooxidans

Comparison of biooxidation with carbon dioxide assimilation during bacterial growth on ferrous ion or elemental sulfur

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