Improved Experimental and Computational Methodology for Determining the Kinetic Equation and the Extant Kinetic Constants of Fe(II) Oxidation by<i>Acidithiobacillus ferrooxidans</i>

Molchanov, Sharon; Gendel, Youri; Ioslvich, Ilya; Lahav, Ori

doi:10.1128/aem.01521-06

Cited by 23 publications

(11 citation statements)

References 42 publications

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“…Table 1 presents the ferrous iron oxidation rate, the specific growth rate as well as the yield as a function of initial ferrous iron concentration. The change in biomass concentration can be related to yield (Y) as follows [13]:…”

Section: Resultsmentioning

confidence: 99%

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Piña

Oliveira

Cruz

et al. 2010

Biochemical Engineering Journal

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The biological oxidation of ferrous iron is an important sub-process in the bioleaching of metal sulfides and other bioprocesses such as the removal of H 2 S from gases, the desulfurization of coal and the treatment of acid mine drainage (AMD). As a consequence, many Fe(II) oxidation kinetics studies have mostly been carried out with mesophilic microorganisms, but only a few with moderately thermophilic microorganisms. In this work, the ferrous iron oxidation kinetics in the presence of Sulfobacillus thermosulfidooxidans (DSMZ 9293) was studied. The experiments were carried out in batch mode (2L STR) and the effect of the initial ferrous iron concentration (2-20 g L −1) on both the substrate consumption and bacterial growth rate was assessed. The Monod equation was applied to describe the growth kinetics of this microorganism and values of max and K s of 0.242 h −1 and 0.396 g L −1 , respectively, were achieved. Due to the higher temperature oxidation, potential benefits on leaching kinetics are forecasted.

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Section: Resultsmentioning

confidence: 99%

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Piña

Oliveira

Cruz

et al. 2010

Biochemical Engineering Journal

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“…Sulfuric acid produced by microbial oxidation was analyzed by titration with 0.01 mol L −1 sodium hydroxide solution. The specific growth rate (μ) of strains tested was evaluated from the exponential phases by nonlinear regression of growth yield ( Y X/S ) vs. substrate oxidation rate (Gourdon & Funtowicz, 1998; Ceskova et al , 2002; Molchanov et al , 2007).…”

Section: Methodsmentioning

confidence: 99%

Genomic and phenotypic heterogeneity of Acidithiobacillus spp. strains isolated from diverse habitats in China

Bao

et al. 2008

FEMS Microbiology Ecology

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The genetic variability among 32 Chinese Acidithiobacillus spp. environmental isolates and four reference strains representing three recognized species of the genus Acidithiobacillus was characterized by using a combination of molecular methods, namely restriction fragment length polymorphisms of PCR-amplified 16S rRNA genes and 16S-23S rRNA gene intergenic spacers, repetitive element PCR, arbitrarily primed PCR and 16S rRNA gene sequence analyses. 16S rRNA gene sequences revealed that all Acidithiobacillus spp. strains could be assigned to seven groups, three of which encompassed the Acidithiobacillus ferrooxidans strains from various parts of the world. A comparative analysis of the phylogenetic Group 1 and 2 was undertaken. Restriction fragment length polymorphism results allowed us to separate the 35 Acidithiobacillus strains into 15 different genotypes. An integrated phenotypic and genotypic analysis indicated that the distribution of A. ferrooxidans strains among the physiological groups were in agreement with their distribution among the genomic groups, and that no clear correlation was found between the genetic polymorphism of the Acidithiobacillus spp. strains and either the geographic location or type of habitats from which the strains were isolated. In addition, five unidentified sulfur-oxidizing isolates may represent one or two novel species of the genus Acidithiobacillus. The results showed that the Chinese Acidithiobacillus spp. isolates exhibited a high degree of genomic and phenotypic heterogeneity.

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“…These higher transmission rates are caused by the increased likelihood of susceptible cattle coming in contact with high levels of bacteria, since as little as 300 CFU is sufficient to infect calves [ 13 ]. Thus, it is plausible that an animal excreting large numbers of O157 will pose a greater risk of spreading the pathogen to other cattle and into the food supply than the combined output of many animals that shed the pathogen at low levels [ 14 , 15 ]. Mathematical modeling also suggests that the spread of O157 could be significantly reduced, if colonization in 5% of super-shedding animals was prevented [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Super-Shedder Strains of Escherichia coli O157:H7 Reveals Distinctive Genomic Features and a Strongly Aggregative Adherent Phenotype on Bovine Rectoanal Junction Squamous Epithelial Cells

et al. 2015

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Shiga toxin-producing Escherichia coli O157:H7 (O157) are significant foodborne pathogens and pose a serious threat to public health worldwide. The major reservoirs of O157 are asymptomatic cattle which harbor the organism in the terminal recto-anal junction (RAJ). Some colonized animals, referred to as “super-shedders” (SS), are known to shed O157 in exceptionally large numbers (>104 CFU/g of feces). Recent studies suggest that SS cattle play a major role in the prevalence and transmission of O157, but little is known about the molecular mechanisms associated with super-shedding. Whole genome sequence analysis of an SS O157 strain (SS17) revealed a genome of 5,523,849 bp chromosome with 5,430 open reading frames and two plasmids, pO157 and pSS17, of 94,645 bp and 37,446 bp, respectively. Comparative analyses showed that SS17 is clustered with spinach-associated O157 outbreak strains, and belongs to the lineage I/II, clade 8, D group, and genotype 1, a subgroup of O157 with predicted hyper-virulence. A large number of non-synonymous SNPs and other polymorphisms were identified in SS17 as compared with other O157 strains (EC4115, EDL933, Sakai, TW14359), including in key adherence- and virulence-related loci. Phenotypic analyses revealed a distinctive and strongly adherent aggregative phenotype of SS17 on bovine RAJ stratified squamous epithelial (RSE) cells that was conserved amongst other SS isolates. Molecular genetic and functional analyses of defined mutants of SS17 suggested that the strongly adherent aggregative phenotype amongst SS isolates is LEE-independent, and likely results from a novel mechanism. Taken together, our study provides a rational framework for investigating the molecular mechanisms associated with SS, and strong evidence that SS O157 isolates have distinctive features and use a LEE-independent mechanism for hyper-adherence to bovine rectal epithelial cells.

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Improved Experimental and Computational Methodology for Determining the Kinetic Equation and the Extant Kinetic Constants of Fe(II) Oxidation byAcidithiobacillus ferrooxidans

Cited by 23 publications

References 42 publications

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Genomic and phenotypic heterogeneity of Acidithiobacillus spp. strains isolated from diverse habitats in China

Comparative Analysis of Super-Shedder Strains of Escherichia coli O157:H7 Reveals Distinctive Genomic Features and a Strongly Aggregative Adherent Phenotype on Bovine Rectoanal Junction Squamous Epithelial Cells

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