The fecal arsenic excretion, tissue arsenic accumulation, and metabolomics analysis in sub-chronic arsenic-exposed mice after in situ arsenic-induced fecal microbiota transplantation

Luo, Yu; Wang, Jiating; Wang, Chenfei; Wang, Dongbin; Li, Chengji; Zhang, Bin; Zhong, Xiaoni; Chen, Linkang; Li, Hao; Su, Haijun; Zheng, Qiuyi; Zhu, Dajian; Tang, Huanwen; Guo, Lianxian

doi:10.1016/j.scitotenv.2022.158583

Cited by 5 publications

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“…The microbiota was characterized by 16S rRNA gene sequencing at the V4–V5 hypervariable region and analyzed as described previously. , Raw data sequences of bacteria were deposited in the NCBI Sequence Read Archive under the Bioproject ID PRJNA905175 and PRJNA970036 (). After acquiring the normalized operational taxonomic units (OTUs), microbial analyses (Supporting Information Note 1) were conducted referring to our previous study …”

Section: Methodsmentioning

confidence: 99%

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Effects of Intestinal Microbiota on the Biological Transformation of Arsenic in Zebrafish: Contribution and Mechanism

Zhong,

Zhang,

Huang

et al. 2024

Environ. Sci. Technol.

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Both the gut microbiome and their host participate in arsenic (As) biotransformation, while their exact roles and mechanisms in vivo remain unclear and unquantified. In this study, as3mt −/− zebrafish were treated with tetracycline (TET, 100 mg/ L) and arsenite (iAs III ) exposure for 30 days and treated with probiotic Lactobacillus rhamnosus GG (LGG, 1 × 10 8 cfu/g) and iAs III exposure for 15 days, respectively. Structural equation modeling analysis revealed that the contribution rates of the intestinal microbiome to the total arsenic (tAs) and inorganic As (iAs) metabolism approached 44.0 and 18.4%, respectively. Compared with wild-type, in as3mt −/− zebrafish, microbial richness and structure were more significantly correlated with tAs and iAs, and more differential microbes and microbial metabolic pathways significantly correlated with arsenic metabolites (P < 0.05). LGG supplement influenced the microbial communities, significantly up-regulated the expressions of genes related to As biotransformation (gss and gst) in the liver, down-regulated the expressions of oxidative stress genes (sod1, sod2, and cat) in the intestine, and increased arsenobetaine concentration (P < 0.05). Therefore, gut microbiome promotes As transformation and relieves As accumulation, playing more active roles under iAs stress when the host lacks key arsenic detoxification enzymes. LGG can promote As biotransformation and relieve oxidative stress under As exposure.

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Section: Methodsmentioning

confidence: 99%

“…After acquiring the normalized operational taxonomic units (OTUs), microbial analyses (Supporting Information Note 1) were conducted referring to our previous study. 42 2.6. RT-qPCR Analyses.…”

Section: Methodsmentioning

confidence: 99%