The feathering gene is linked to degranulation and oxidative burst not cytokine/chemokine mRNA expression levels or<i>Salmonella enteritidis</i>organ invasion in broilers

Swaggerty, Christina L.; He, Haiqi; Genovese, Kenneth J.; Kaiser, Pete; Pevzner, I. Y.; Kogut, Michael H.

doi:10.1080/03079450601028829

Cited by 8 publications

(1 citation statement)

References 38 publications

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“…Redmond and colleagues proved heterophils from commercially selected and non-selected genetic lines expressed cytokines differently in vitro exposure to Salmonella enteritidis, where functional phenotypes of phagocytosis and bacterial killing varied depending on the breed or cross of chicken and the SLC11A1 gene was associated with phagocytosis of Salmonella enteritidis [22]. Swaggerty demonstrated genes associated with fast and slow feathering located on the Z sex chromosome were associated with the biochemical killing mechanism of degranulation and oxidative burst of heterophil in avian species [24]. This study found the phagocytosis and oxidative burst of heterophils isolated from selection lines performed more powerfully compared to the non-selection line, indicating heterophil function related genes were selected under the H/L ratio selection pressure.…”

Section: Discussionmentioning

confidence: 99%

Immunogenetic Basis of Chicken’s Heterophil/Lymphocyte Ratio Revealed by Genome-wide Indel Variants Analysis

Zhao

Zhang

Wang

et al. 2022

Preprint

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BackgroundEnhancing host immunity is an effective way to reduce morbidity in chickens. Heterophil/lymphocyte (H/L) ratio is associated with host disease resistance in birds. Chickens with different H/L ratio levels show different disease resistances, caused by a difference in the functional capacity of heterophils. In this study, an H/L directional breeding chicken line was constructed and a combined statistical analysis was used to explore the candidate genes and pathways related to H/L ratio based on whole-genome sequencing of 368 individuals, divided into a non-selection line (NS) and selection lines including G5, G9 and F2 of G9.ResultsHeterophils isolated from the H/L selection line had stronger phagocytosis and oxidative burst function than non-selection line. The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9 compared to NS based on a genome-wide selective sweep. Several selective regions were identified containing genes like interferon induced with helicase C domain 1 (IFIH1) and moesin (MSN) associated with the intracellular receptor signaling pathway, C-C motif chemokine receptor 6(CCR6)、dipeptidyl peptidase 4(DPP4) and hemolytic complement (HC) associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1) associated with actin cytoskeleton organization. In addition, 45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L ratio based on genome-wide association study (GWAS). The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5) and oxysterol binding protein like 5(OSBPL5) were positively correlated with H/L ratio and significantly different between the high and low H/L groups of the Beijing-You chicken. The A/A allelic frequency of indel 5_13108985 (P-value= 3.85E-06) within OSBPL5 gradually increased from the non-selection line to the G5 and G9 and the individuals with A/A exhibited lower H/L ratio. ConclusionsThe phagocytosis and oxidative burst functions were enhanced and 22.44 Mb genomic regions were selected with the directional selection of H/L ratio. PTPN5 and OSBPL5 genes were identified as candidate genes. These findings revealed the complex genetic mechanism of H/L ratio related to immunity and will allow selection for improving chicken immunity based on the H/L ratio.

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Section: Discussionmentioning

confidence: 99%

Immunogenetic Basis of Chicken’s Heterophil/Lymphocyte Ratio Revealed by Genome-wide Indel Variants Analysis

Zhao

Zhang

Wang

et al. 2022

Preprint

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The different expression of immune-related cytokine genes in response to velogenic and lentogenic Newcastle disease viruses infection in chicken peripheral blood

et al. 2011

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Newcastle disease virus (NDV) is an important pathogen hazardous to poultry industry, and the pathogenicity of NDV strains varies with different virulence. Peripheral blood serves as an important producer and carrier of viruses and cytokines in NDV infection. In order to explore the difference of cytokine expression in the peripheral blood between velogenic strain and lentogenic strain infection, NDV virulent strain F48E9 and vaccine strain Lasota were used to infect specific-pathogen-free (SPF) chickens separately, and peripheral blood was collected on 0, 3, 7, 10, 14, and 21 days post-infection (d.p.i.). Real-time PCR was then used to detect the expression of six kinds of immune-related cytokine genes. For the F48E9 group, a sharp increase of the expression of interferon-alpha (IFN-α), interferon-gamma (IFN-γ), interleukin-16 and IL-18 was observed on 3 d.p.i. before the NDV blood peak (7 d.p.i.), followed by a rapid decline to the level lower than that of control group, then the expression of IFN-α increased slowly and reached or exceeded the level of control group in the later phase of the infection, while the expression of IFN-γ, IL-16, and IL-18 fluctuated at the level of control group for the rest of study period. The increase of IL-2 expression was not obvious, and no increase of IL-15 expression was noted. For the Lasota (vaccine) group, the picture was quite different, a sharp increase of IFN-γ (but not IFN-α), IL-2 was observed on 7 d.p.i. before the NDV blood peak (10 d.p.i.). On the contrary, there was no dramatic increase of IL-16 and IL-18. Interestingly, in contrast to the F48E9 group, there was an increase of IL-15 on day 10 d.p.i., but it remained modest. There was also an increase of IFN-α on day 21 d.p.i. Our results revealed that infection with NDV strains of different virulence was associated with distinct cytokine expression patterns in peripheral blood, modulation of cytokine responses may play a key role in mediation of NDV pathogenesis.

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The avian heterophil

Genovese

Swaggerty

et al. 2013

Developmental & Comparative Immunology

120

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The feathering gene is linked to degranulation and oxidative burst not cytokine/chemokine mRNA expression levels orSalmonella enteritidisorgan invasion in broilers

Cited by 8 publications

References 38 publications

Immunogenetic Basis of Chicken’s Heterophil/Lymphocyte Ratio Revealed by Genome-wide Indel Variants Analysis

Immunogenetic Basis of Chicken’s Heterophil/Lymphocyte Ratio Revealed by Genome-wide Indel Variants Analysis

The different expression of immune-related cytokine genes in response to velogenic and lentogenic Newcastle disease viruses infection in chicken peripheral blood

The avian heterophil

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