The fatty acid compositions of phospholipids and triacylglycerols from two chinch bug species Blissus leucopterus leucopterus and B. iowensis (Insecta: Hemiptera; Lygaeidae) are similar to the characteristic dipteran pattern

Spike, B. P.; Wright, Robert J.; Danielson, Stephen D.; Stanley-Samuelson, David W.

doi:10.1016/0305-0491(91)90144-3

Cited by 19 publications

(13 citation statements)

References 15 publications

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“…Dipterans are characteristically high in C16:1 (Fast 1970). The dipteran pattern of high C16:1 was also seen in four heteropterans: Blissus leucopterus leucopterus, Blissus iowensis (Spike et al 1991), Eurygaster maura L. (Kilincer et al 1987) and Eurygaster integriceps (Bashan et al 2002). However, proportion of C16:1 is low in most Heteroptera (Thompson 1973;Bashan & Cakmak 2005).…”

Section: Discussionmentioning

confidence: 99%

Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages

2007

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Total lipid and the fatty acid compositions of phospholipid and triacylglycerol fractions, prepared from eggs, 3 rd instars of larvae, pupae, male and female adults of Lertha sheppardi, were analyzed by gas chromatography and gas chromatography-mass spectrometry. The effect of diet (adults' nutrition) on fatty acid composition of L. sheppardi adults was also investigated. Total lipid of L. sheppardi considerably increased in adults compared with immature stages. There was a significant decrease in total lipid level in larval stage in contrast with egg stage. Qualitative analysis revealed the presence of 14 fatty acids during all stages. The major components were C16 and C18 saturated and unsaturated components which are ubiquitous to most animal species. In addition to these components, one odd-chain (C17:0) and prostaglandin precursor fatty acids were found. The fatty acid profiles of phospholipids and triacylglycerols were substantially different. In phospholipid fraction, monounsaturated fatty acids were the major proportion of fatty acids in both sex of adults and pupae, whereas polyunsaturated fatty acids were the most dominant fatty acids in eggs and 3 rd instars. Results of triacylglycerol fraction revealed that fatty acid composition of eggs had higher level of C16:1, C18:0 and C18:3n-3 content than that of 3 rd instars and pupae, which suggests accumulation of energetic and structural reserve materials during embryonic development. At more advanced developmental stages, mainly in adult females, the amount of C16:1 increased once again, which may be related to the need for accumulation of sufficient energy and of carbon reservoir in the developing new vitellum. Percentages of C18:1 were significantly high in adult stages compared to other stages. These findings indicate that the accumulation and consumption of fatty acids fluctuate through different development stages. Diet did not effect the fatty acid composition of L. sheppardi adults.

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Section: Discussionmentioning

confidence: 99%

Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages

2007

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“…Insects contain a ∆ 9 desaturase that desaturates 16:0 and 18:0 fatty acids to ∆ 9 monounsaturates (Stanley-Samuelson et al, 1988); however, for most insects the quantities of 16:1 are minor compared to 18:1 (Downer, 1985;StanleySamuelson et al, 1988). High proportions of 16:1 fatty acids have also been found in some Heteroptera (Spike et al, 1991;Bashan et al, 2002). For the total lipids from Homoptera, amounts of 16:1 were minor in aphids, ranging between 0.6-2.6% (Dillwith et al, 1993) and, for the internal lipids of nymph and adult whiteflies, 16:1 amounts were only 1-2% (Buckner and Hagen, 2003).…”

Section: September 2004mentioning

confidence: 99%

Characterization of triacylglycerols from overwintering prepupae of the alfalfa pollinator Megachile rotundata (Hymenoptera: Megachilidae)

Buckner¹,

Kemp²,

Bosch³

2004

Arch Insect Biochem Physiol

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Alfalfa leafcutting bees, Megachile rotundata (F.), overwinter as prepupae. The internal lipids were extracted from prepupae that had been wintered at 4°C for 7 months. Megachile rotundata prepupae possessed copious quantities of internal lipids (20% of the fresh weight) that were extracted with CHCl 3 /methanol (2:1). Transmission electron microscopy revealed that lipids were stored within very large intracellular vacuoles. Separation by silica chromatography revealed that 88% of the internal lipids were triacylglycerols. Ester derivatives of fatty acids from triacylglycerol components were analyzed by gas chromatography-mass spectrometry and 15 fatty acid constituents were identified. The majority (76%) of the triacylglycerol fatty acids were unsaturated fatty acids. The major triacylglycerol fatty acid constituent (30%) was the C 16 monounsaturated fatty acid, palmitoleic acid (16:1, hexadec-9-enoic acid), with substantial amounts of linolenic acid (18:3, octadec-9,12,15-trienoic acid, 15%), palmitic acid (16:0, hexadecanoic acid, 14%) and oleic acid (18:1, octadec-9-enoic acid, 13%). Palmitoleic acid as the major fatty acid of an insect is an unusual occurrence as well as the presence of the 16-carbon polyunsaturated fatty acids, 16:2 and 16:3. The major intact triacylglycerol components were separated and identified by high performance liquid chromatography-mass spectrometry. A complex mixture of approximately 40 triacylglycerol components were identified and major components included palmitoyl palmitoleoyl oleoyl glycerol, palmitoyl palmitoleoyl palmitoleoyl glycerol, myristoyl palmitoleoyl palmitoleoyl glycerol, myristoleoyl palmitoyl palmitoleoyl glycerol, and palmitoyl palmitoleoyl linolenoyl glycerol. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. U.S. Department of Agriculture, Agricultural Research Service, Northern Plains Area, is an equal opportunity/ affirmative action employer and all agency services are available without discrimination.Abbreviations used: BSA = N,O-bis-(trimethylsilyl) acetamide; FAME = fatty acid methyl ester; L = linoleic acid; La = lauric acid; Ln = linolenic acid; M = myristic acid; Mo = myristoleic acid; O = oleic acid; P = palmitic acid; Po = palmitoleic acid; P L = palmitlinoleic acid; P Ln = palmitlinolenic acid; S = stearic acid; TAG = triacylglycerol; t-BME = tert-butylmethylether; TMS = trimethylsilyl.

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“…This component was only found in high percentages in diptera (Thompson, 1973), some heteroptera (Spike et al, 1991;Bashan et al, 2002) and in diatoms (Kharlamenko et al, 1995). We obtained C16:1ω7 acid 30.2% in the gills and 27.6% in whole body of U. elongatulus.…”

Section: Discussionmentioning

confidence: 68%

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Ekin¹,

Başhan²

2010

Turk. J. Fish. Aquat. Sci.

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The total lipid, fatty acid content of some organs and whole specimen of freshwater mussel Unio elongatulus were investigated. The mussels were collected in July in 2007 from Tigris River, Turkey. Fatty acid content of selected tissues and whole mussel was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In the analyses, predominant fatty acids were C16:0, C16:1ω7, C18:1ω9, C20:1ω9, C20:4ω6 and C20:5ω3. Analyses of selected organs (mantle, gills, foot and whole body) presented different and characteristic fatty acids profiles. In the gills C16:1ω7 (30.2%), C16:0 (17.4%) acids; in the mantle C18:1ω9 (20.3%), C16:0 (25.4%) acids; in the foot C16:0 (20.8%), C16:1ω7 (15.9%), C18:1ω9 (15.4%) acids and in whole body C16:1ω7 (27.6%), C16:0 (23.6%) acids were the most abundant constituents. Also the percentages of C20:4ω6 and C20:5ω3 acids, precursors of eicosanoids, were apparently high in the gills and foot. It is presumed that the chief fatty acids present in a particular organ are related to specific functions of the organ. In all of the tissue analyses, ΣMUFA levels were higher than ΣPUFA and ΣSFA levels. Midyenin bütün vücut dokusu ile seçilmiş organlarının yağ asidi içeriği gaz kromatografi (GC) ve gaz kromatografi-kütle spektrometre (GC-MS) ile analiz edildi. Analizlerde, C16:0, C16:1ω7, C18:1ω9, C20:1ω9, C20:4ω6 ve C20:5ω3 asitler yoğunlukta bulunan bileşenlerdi. Analiz edilen organlar (manto, solungaç, ayak ve tüm vücut) farklı ve karakteristik yağ asidi profili gösterdi. Solungaçta C16:1ω7 (%30,2), C16:0 (%17,4) asitler; mantoda C18:1ω9 (%20,3), C16:0 (%25,4) asitler; ayakta C16:0 (%20,8), C16:1ω7 (%15,9), C18:1ω9 (%15,4) asitler ve tüm vücut dokusunda ise C16:1ω7 (%27,6), C16:0 (%23,6) asitler en çok bulunan bileşenlerdi. Ayrıca eikosanoidlerin öncül maddesi olan C20:4ω6 ve C20:5ω3 asitlerin yüzde oranları, solungaç ve ayakta önemli oranda yüksek bulundu. Belirli organlardaki temel yağ asitlerinin organların spesifik fonksiyonları ile bağlantılı olduğu sanılmaktadır. Tüm doku analizlerinde, ΣTDYA oranı, ΣDYA ve ΣÇDYA oranlarından daha yüksekti.Anahtar Kelimeler: Yağ asitleri, tatlısu midyesi, Unio elongatulus, Dicle Nehri.

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The fatty acid compositions of phospholipids and triacylglycerols from two chinch bug species Blissus leucopterus leucopterus and B. iowensis (Insecta: Hemiptera; Lygaeidae) are similar to the characteristic dipteran pattern

Cited by 19 publications

References 15 publications

Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages

Total lipid and fatty acid compositions of Lertha sheppardi (Neuroptera: Nemopteridae) during its main life stages

Characterization of triacylglycerols from overwintering prepupae of the alfalfa pollinator Megachile rotundata (Hymenoptera: Megachilidae)

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