Chilika buffaloes, recognized as a breed since 2009 vide accession no. India_Buffalo_1500-Chilika_01012, are found in and around Chilika lake of Odisha. These buffaloes are reared under purely extensive system with no feed and medicine supplementation. The present study was undertaken to know the feeding pattern, metabolic status and milk composition of Chilika buffaloes in their natural habitat as very few literature is available about these buffaloes which are reared with zero external input system. The major portion of their feed is composed of different brackishwater weeds growing in and around the lake and grasses growing in the shallow water beds. The study was undertaken taking 60 buffaloes in their first to fourth lactation from 3 villages. The proximate composition of weeds and grasses were comparable with a good quality fodder but having higher acid insoluble ash. From the serum metabolic status it was seen that the protein level was adequate in the animals but the glucose level below normal range. These buffaloes have optimum serum level of major and minor minerals except Na, K and Fe, which is higher than the normal values. The milk fat content was higher than other buffaloes. It was found that among the PUFA the ratio of n3 to n6 was quite good which was higher than cattle and comparable with the fish. Further research is required to explore the availability of the biomass and exploit the potentiality of these buffaloes for the rich n:3 fatty acid content in the milk to be utilized by the today's health conscious society.