THE FATE OF Cr⁵¹ LABELLED EHRLICH ASCITES TUMOUR CELLS

Abstract

Summary Elution of Na2Cr51O4 after binding to Ehrlich ascites tumour cells is considerable, both in vitro and in vivo. The distribution and excretion of intravenously injected free Na2Cr51O4 have been determined and with these data it has been estimated that approximately 64 per cent of isotope associated with tumour cells is lost in the first 24 hours after their intravenous injection into mice. Most (but not all) of the activity in the liver and spleen, and all of the activity in the kidneys after such an injection is due to the distribution of eluted isotope. However, a significant excess of radioactivity in the lungs of female mice when compared with male mice within one minute of injection of labelled cells is consistent with previous reports of greater metastatic tumour growth in female mice. Evidence is presented to show that clution of isotope is not responsible for this difference.

Ultrastructure of the Adhesion of HeLa Cells to Human Vein Wall

“…A constant release o f cells from the pulmonary into the systemic circulation occurred, and there was accumulation of about 20% of the cells injected in the liver at the end of the first six hours. The binding o f radioactive chromium to the Ehrlich ascites tumour cells is not as durable as had first been thought and there is elution of approximately 64% o f the isotope from its bound form in the cell within the first 24 h in vivo [32].…”

A convenient route for the synthesis of some new bi- and triheterocondensed uracils

“…Also, the compounds were tested against fungus (Candida albicans) in comparison with nystatin as an antifungal standard agent using a filter paper disc method [32,33]. The results are recorded in Table 1.…”

Death and Metastatic Distribution of Tumor Cells in Mice Monitored With ¹²⁵I-Iododeoxyuridine<xref ref-type="fn" rid="FN2">2</xref>

“…It also should not damage the cells and should be rapidly excreted from the host when the labeled cells die. Attempts to study the fate of tumor cells by labeling with 32p (7) or 51Cr (8)(9)(10) have been only partially successful, since a fraction of either isotope can exchange out of the living cell and because both isotopes are largely reutilized by surviving tumor cells or normal host cells after death and breakdown of labeled cells. This produces a progressively larger error when the fate and distribution of tumor cells are studied over extended periods (11).…”