The Fate of Bacteria Sealed in Dental Cavities

Besic, F.C.

doi:10.1177/00220345430220050101

Cited by 66 publications

(29 citation statements)

References 2 publications

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“…Similar alterations have been described for the activity of dentinal lesions [Miller and Massler, 1962;Massler, 1967] as well as for root surface lesions [Nyvad and Fejerskov, 1986;Schüp-bach et al, 1990]. In accordance with studies on stepwise excavation procedures applying a rigorous first excavation step [Besic, 1943;Schouboe and Macdonald, 1962;King et al, 1965;Aponte et al, 1966;Fisher, 1969], we also obtained a marked reduction in bacterial growth following the treatment interval [Bjørndal et al, 1997]. However, in some lesions, a growth of streptococci and lactobacilli was still detected.…”

supporting

confidence: 74%

Changes in the Cultivable Flora in Deep Carious Lesions following a Stepwise Excavation Procedure

Bjørndal

Larsen²

2000

Caries Res

164

109

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This study examined the cultivable microflora before and after stepwise excavation procedures in deep carious lesions in 9 permanent teeth, categorized according to degrees of proximal surface destruction. The final excavation was performed 4–6 months after the initial treatment, which included peripheral dentine excavation and removal of the central cariogenic biomass and the superficial necrotic dentine. Dentine colour and consistency were assessed by means of standardized scales before the application of a Ca(OH)₂ compound and temporary sealing. Reassessments were performed before and after the final excavation. Microbiological samples of the central demineralized dentine were obtained with a sterile bur before and after the first excavation, as well as before and after the final excavation. After anaerobic cultivation on enriched non–selective tryptic soy agar, 30 colonies from a representative area were identified by standardized biochemical and physiological tests. Before temporary restoration, a yellowish and light brown demineralized soft dentine was typically observed, and gram–positive rods accounted for 70% and lactobacilli for 50% of the total colony–forming units. Lactobacillus casei subsp. rhamnosus and Actinomyces naeslundii dominated the lactobacilli and the other gram–positive rods, respectively. Gram–negative rods were the next most frequent isolates, followed by streptococci, each group accounting for about 20% of the colony–forming units in positive samples. Before the final excavation, which did not cause exposure of the pulp in any of the cases, the retained demineralized dentine had changed into a darker and harder tissue, and the total colony–forming units, as well as the frequency and proportions of lactobacilli were substantially reduced. Gram–negative rods also declined, and the flora was dominated by A. naeslundii and various streptococci. In conclusion, the cultivable flora detected following the treatment interval had declined substantially, and the distribution of bacterial species did not represent a typical cariogenic microbiota of deep lesions, confirming the clinical findings of arrested caries progression.

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supporting

confidence: 74%

Changes in the Cultivable Flora in Deep Carious Lesions following a Stepwise Excavation Procedure

Bjørndal

Larsen²

2000

Caries Res

164

109

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“…Since cavity preparation usually involves some loss of healthy dental tissue, alternative approaches have been proposed to preserve tooth structure, such as the sealing of carious tissue beneath sealants or restorations. These approaches may even reduce or completely eliminate the population of viable microorganisms, 1,2,3,4,5,6,7,8 thus controlling caries progression.…”

Section: Introductionmentioning

confidence: 99%

A randomized clinical trial on the sealing of occlusal carious lesions: 3–4-year results

et al. 2017

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This randomized clinical trial aimed to assess the efficacy of sealing occlusal carious lesions in permanent teeth. The sample consisted of 54 occlusal carious lesions in permanent molars and premolars of 49 patients aged 8-43 years (median: 19 years). The inclusion criteria comprised the presence of a cavity with no access allowing biofilm control. The maximum depth of the lesion was the middle third of the dentin thickness, as assessed by bitewing radiography. The teeth were randomly assigned to sealant treatment (n = 28) or restorative treatment (n = 26). Clinical and radiographic examinations were performed after 1 year and after 3-4 years. The outcomes depended on the clinical performance of the sealant/restoration and the control of caries progression observed radiographically. Survival analysis was performed to assess success rates. Over the 3-4 years of monitoring, 2 sealants were totally lost, 1 needed repair, and 1 showed caries progression, totaling 4 failures in the sealant group. In the restoration group, 1 failure was observed (in need of repair). The success rates were 76% and 94% in the sealant and the restoration groups, respectively (p > 0.05). The sealing of occlusal carious lesions in permanent teeth succeeded in controlling caries over a 3-4-year period. However, sealed carious lesions require patient compliance in attending regular follow-ups to control the occurrence of clinical failures of the sealants.

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“…Histological and bacteriologic studies have shown that only a small proportion of the teeth are sterile after cavity preparation. A number of studies have demonstrated that the bacteria left in the dentin of a cavity preparation could maintain their activity for a long time 6 .…”

Section: Introductionmentioning

confidence: 99%

Topic-the effect of three cavity disinfectants (chlorhexidine gluconate-based. Consepsis; benzalkonium chlorite-based, Tubulicid red; sodium hypochlorite based-Chlorcid V on the self - etch dentine bonding agent ( Adeper Easy One,3M ESPE ) under SEM.

Pattanaik¹,

Chandak²

2013

IOSR-JDMS

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The Fate of Bacteria Sealed in Dental Cavities

Cited by 66 publications

References 2 publications

Changes in the Cultivable Flora in Deep Carious Lesions following a Stepwise Excavation Procedure

Changes in the Cultivable Flora in Deep Carious Lesions following a Stepwise Excavation Procedure

A randomized clinical trial on the sealing of occlusal carious lesions: 3–4-year results

Topic-the effect of three cavity disinfectants (chlorhexidine gluconate-based. Consepsis; benzalkonium chlorite-based, Tubulicid red; sodium hypochlorite based-Chlorcid V on the self - etch dentine bonding agent ( Adeper Easy One,3M ESPE ) under SEM.

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