The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer

Fu, Xiaoling; Liu, Yangyang; Qiu, Ruiyun; Foda, Mohamed F.; Zhang, Yong; Wang, Tao; Li, Jinshan

doi:10.1016/j.bbrep.2018.01.002

Cited by 15 publications

(10 citation statements)

References 19 publications

(19 reference statements)

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“…As above method, all urine samples were detected, 3 Journal of Nanomaterials respectively, using previous and new LSPR biosensors. Meanwhile, same clinical samples were confirmed using the CLIA (Chemiluminescence Immunoassay) method according to the manufacturer's instructions [14]. The detection results were collected and analyzed.…”

Section: Detection Of He4 In Urinementioning

confidence: 99%

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A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

Duan

2020

Journal of Nanomaterials

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A non-invasive and more sensitive method for detection of HE4 is very important for the early screening and detection of ovarian carcinoma. In this study, we improved our previous localized surface plasmon resonance (LSPR) biosensor for detection of HE4 in urine to overcome disadvantages of conventional methods. Protein G directional fixed method was firstly used for LSPR biosensor to improved sensitivity, and standard HE4 and clinical samples were detected separately using this new biosensor. Compared to our previous LSPR biosensor, this new sensor was more sensitive, with other advantages as before. Under optimum conditions, this new biosensor could display a detection limit of 1 pM and wide dynamic range of 1 pM to 10,000 pM. This new biosensor was effective for detection of HE4 in urine of early ovarian cancer patients, without label and purification. To the best of our knowledge, this is first work to investigate LSPR biosensor for detection of tumor marker in urine, with great advantages and clinical application potentials.

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Section: Detection Of He4 In Urinementioning

confidence: 99%

“…HE4 detection in urine of different study groups with CLIA method (based on Chemiluminescence Immunoassay method[14]). …”

mentioning

confidence: 99%

A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

Duan

2020

Journal of Nanomaterials

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“…Human epididymis protein 4 is a new biomarker approved by the Food and Drug Administration, which has received considerable attention owing to its capacity for the diagnosis of epithelial ovarian cancer [90]. By coating magnetic particles with alkaline phosphatase antibody, a sensitive and specific method for the determination of human epididymis protein 4 by chemiluminescence immunoassay has been developed [91]. Furthermore, a novel method for the separation and detection of circulating ovarian cancer cells using whole blood samples has been demonstrated.…”

Section: Cancer Diagnosismentioning

confidence: 99%

Magnetic Particles for Advanced Molecular Diagnosis

2019

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Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

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“…Protein G is covalently immobilized on the silver nanoparticles, and an anti-HE4 antibody is attached to protein G. Such a biosensor is suitable for the determination of the HE4 marker in urea. Recently, a molecularly-imprinted photo-electrochemical sensor was developed for HE4 determination [10], as was a chemiluminescence immunoassay with HE4 accumulation on magnetic particles [11]. The aim of the present paper was to develop a much simpler, label-free immunosensor for HE4 determination based on a single antibody and the SPRi technique.…”

Section: Introductionmentioning

confidence: 99%

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

Szymańska

Łukaszewski

Żelazowska-Rutkowska

et al. 2021

Sensors

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Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of rabbit polyclonal antibody against HE4, covalently attached to a gold chip via cysteamine linker. The biosensor is used with the non-fluidic array SPRi technique. The linear range of the analytical signal response was found to be 2–120 pM, and the biosensor can be used for the determination of the HE4 marker in the plasma of both healthy subjects and ovarian cancer patients after suitable dilution with a PBS buffer. Precision (6–10%) and recovery (101.8–103.5%) were found to be acceptable, and the LOD was equal to 2 pM. The biosensor was validated by the parallel determination of a series of plasma samples from ovarian cancer patients using the Elecsys HE4 test and the developed biosensor, with a good agreement of the results (a Pearson coefficient of 0.989). An example of the diagnostic application of the developed biosensor is given—the influence of ovarian tumor resection on the level of HE4 in blood serum.

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The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer

Cited by 15 publications

References 19 publications

A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

Magnetic Particles for Advanced Molecular Diagnosis

An SPRi Biosensor for Determination of the Ovarian Cancer Marker HE4 in Human Plasma

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