The Fabrication of a Gellan Gum-Based Hydrogel Loaded With Magnesium Ions for the Synergistic Promotion of Skin Wound Healing

Abstract

To accelerate serious skin burn wound healing in a convenient manner, an interpenetrating network of hydrogel consisting of gellan gum and polyacrylamide was synthesized by chemical crosslinking and Mg2+ ion immersion techniques. The prepared Mg2+@PAM/GG hydrogel was characterized by morphology, water vapor loss, swelling ratio, rheological properties, tensile mechanical, biocompatibility, and flow cytometry study. The results show that Mg2+@PAM/GG hydrogel’s mechanical strength could be enhanced by the dual network structure and physical crosslinking agent Mg2+ ions. In addition, the tension strength of Mg2+@PAM/GG hydrogel is obviously increased from 86 to 392 kPa, the elongation at break increased from 84 to 231%, and crosslinking density N increased from 4.3 to 7.2 mol/m3 compared with pure GG hydrogel. The cumulative release curve of Mg2+ ions shows that the multiple release mechanism of Mg2+ ions belong to non-Fick’s diffusion. Meanwhile, in vitro experiments show that Mg2+@PAM/GG double network hydrogel has favorable proliferation and an NF-κB pathway inhibition property for fibroblast cells. Finally, the healing effect of the Mg2+@PAM/GG was evaluated in a rat full-thickness burn model. The animal study demonstrates that Mg2+@PAM/GG could accelerate the healing efficiency in case of the sustained-released Mg2+ ions in wound beds. Considering this excellent performance, this convenient prepared hydrogel has great potential as a commercial application for skin full-thickness burn healing materials.

A Mg2+/polydopamine composite hydrogel for the acceleration of infected wound healing

“…Together, these results clearly demonstrated that the desirable physicochemical characteristics endowed the PDA-PAM/Mg 2+ hydrogel the best wound healing efficiency. Compared with another Mg 2+ -based hydrogel (21 days) composing of PAM and Gellan Gum (GG), our PDA-PAM/Mg 2+ gel (14 days) still showed a shorter healing time [ 39 ].

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Gellan Gum in Wound Dressing Scaffolds

“…These hydrogels displayed increased tension strength from 86 to 392 kPa and elongation at break from 84 to 231% compared with plain gellan gum hydrogels, indicating good mechanical performance that is significant for wound dressing. The in vivo experiments using the rat full-thickness burn model showed that the gellan gum/polyacrylamide hybrid hydrogels incorporated with magnesium ions significantly accelerated wound closure in the case of the sustained release of magnesium ions in the wound beds [ 72 ]. Ozkahraman et al designed gellan gum-based bilayer hydrogels loaded with an antibiotic, ampicillin.…”

Solvent Casting and UV Photocuring for Easy and Safe Fabrication of Nanocomposite Film Dressings

“…Globally, the obtained results open prospective for the application of photocurable GG derivatives for the fabrication of nanocomposite films with antibacterial properties. GG is only marginally considered in the scenario of the wound dressing materials [39], even if it possesses interesting features to efficiently support wound healing [54][55][56][57].…”