The fabrication and testing of electrospun silica nanofiber membranes for the detection of proteins

Tsou, Pei-Hsiang; Chou, Chao-Kai; Saldana, S. M.; Hung, Mien‐Chie; Kameoka, Jun

doi:10.1088/0957-4484/19/44/445714

Cited by 34 publications

(17 citation statements)

References 19 publications

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“…Nevertheless, the silica fibers are yet quite brittle. Fabrication of silica nanofibers via traditional electrospinning methods has previously been reported (Shin et al, ; Tsou et al, ). For example, tetraethyl orthosilicate (TEOS) has widely been used for fabricating pure silica or silica‐polymer nanofibers via sol–gel electrospinning (Katoch and Kim, ; Pirzada et al, ).…”

Section: Resultsmentioning

confidence: 99%

Manufacturing of bioreactive nanofibers for bioremediation

Tong

Mutlu

Wackett

et al. 2014

Biotech & Bioengineering

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Recombinant Escherichia coli (E. coli) cells were successfully encapsulated in reactive membranes comprised of electrospun nanofibers that have biocompatible polyvinyl alcohol (PVA)-based cores entrapping the E. coli and silica-based, mechanically sturdy porous shells. The reactive membranes were produced in a continuous fashion using a coaxial electrospinning system coupled to a microfluidic timer that mixed and regulated the reaction time of the silica precursor and the PVA solution streams. A factorial design method was employed to investigate the effects of the three critical design parameters of the system (the flow rate of the core solution, protrusion of the core needle, and the viscosity of the core solution) and to optimize these parameters for reproducibly and continuously producing high-quality core/shell nanofibers. The feasibility of using the reactive membranes manufactured in this fashion for bioremediation of atrazine, a herbicide, was also investigated. The atrazine degradation rate (0.24 µmol/g of E. coli/min) of the encapsulated E. coli cells expressing the atrazine-dechlorinating enzyme AtzA was measured to be relatively close to that measured with the free cells in solution (0.64 µmol/g of E. coli/min). We show here that the low cost, high flexibility, water insolubility, and high degradation efficiency of the bioreactive membranes manufactured with electrospinning makes it feasible for their wide-spread use in industrial scale bioremediation of contaminated waters.

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Section: Resultsmentioning

confidence: 99%

Manufacturing of bioreactive nanofibers for bioremediation

Tong

Mutlu

Wackett

et al. 2014

Biotech & Bioengineering

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“…During the past two decades, sensors, especially biosensors, have become necessary for detecting different analytes such as explosives (41,42), proteins (43,44) DNA (45,46), cancer markers (47,48), bacteria (49,50), viruses (51,52) and toxins (53,54) in food processing, environmental monitoring, clinical diagnostics, and the fight against bioterrorism (3,19,20). As a result, there is a pressing need for the development of target-selective sensors.…”

Section: Introductionmentioning

confidence: 99%

Real Time Diagnostic Point of Care by Amperometric Immuno-Biosensor Kit by Flow Technology

Braustein

2014

ECS Trans.

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There is a growing need for virus-detecting sensors with improved sensitivity and dynamic range, for applications including disease diagnosis, pharmaceutical research, agriculture and homeland security. We report a novel electrochemical biosensing method for improving the sensitivity for detection of the bacteriophage virus MS2, using nanoporous oxirane-derivatized beads. These beads are a commercial polymethyl-metacrylate (PMMA) polymer that has extremely high surface area to volume ratio, making it an ideal platform for surface based sensors. We have developed and evaluated a method for covalent bioconjugation of antibodies and biological support to polymeric beads. The resulting Solid State Kits (SSK) were used to selectively capture enzyme-labeled MS2 viruses from different solutions, enabling detection of a viral concentration of as low as 10 plaque-forming units per milliliter (pfu ml -1 ) by measuring the current (A) from the exposed SSK beads to the enzymatic reaction electrons movement not clear. The kit is connected to a "home made" designed micro-flow system, that exhibits sensitivity and dynamic range similar to the ELISA immuno-liquid array-based assay while outperforming protein micro-array methods.Immuno-Amperometric techniques, using nano-Bio-Polymers Solid Phase Disposable Kit, were used to measure and thus to validate the accuracy of novel technology for virus concentration determination. These work demonstrate the utility of immunoelectrochemical techniques for use in environmental-health quality assurance measurements of viruses.

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“…[1-4] In the sensors, the recognition element is either integrated within or is closely associated with a transducer interface that can convert chemical, physical, or biological interactions into a measurable output. During the past two decades, sensors, especially biosensors, have become necessary for detecting different analytes such as explosives,[5,6] proteins,[7,8] DNA,[9,10] cancer markers,[11,12] bacteria,[13,14] viruses,[15,16] and toxins[17,18] in food processing, environmental monitoring, clinical diagnostics, and the fight against bioterrorism. [3,19,20] In terms of the transducer properties, biosensors can be classified as optical, thermometric, piezoelectric, magnetic, micromechanical, and electrochemical.…”

Section: Introductionmentioning

confidence: 99%

Virus‐Based Chemical and Biological Sensing

2009

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Viruses have recently proven useful for the detection of target analytes such as explosives, proteins, bacteria, viruses, spores, and toxins with high selectivity and sensitivity. Bacteriophages (often shortened to phages), viruses that specifically infect bacteria, are currently the most studied viruses, mainly because target-specific nonlytic phages (and the peptides and proteins carried by them) can be identified by using the well-established phage display technique, and lytic phages can specifically break bacteria to release cell-specific marker molecules such as enzymes that can be assayed. In addition, phages have good chemical and thermal stability, and can be conjugated with nanomaterials and immobilized on a transducer surface in an analytical device. This Review focuses on progress made in the use of phages in chemical and biological sensors in combination with traditional analytical techniques. Recent progress in the use of virus—nanomaterial composites and other viruses in sensing applications is also high-lighted.

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The fabrication and testing of electrospun silica nanofiber membranes for the detection of proteins

Cited by 34 publications

References 19 publications

Manufacturing of bioreactive nanofibers for bioremediation

Manufacturing of bioreactive nanofibers for bioremediation

Real Time Diagnostic Point of Care by Amperometric Immuno-Biosensor Kit by Flow Technology

Virus‐Based Chemical and Biological Sensing

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