The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro

Morimoto, Kenichi; Nabeshima, Yukiko; Mochizuki, Mitsuru; Oomizu, Souichi; Yamamoto, Shoso; Hide, Michihiro

doi:10.1007/s00403-005-0636-y

Cited by 3 publications

(3 citation statements)

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“…The levels of εGT in B cells, cultured as described above, were evaluated by quantitative real-time RT-PCR with an ABI PRISM 7700 sequence detector (Applied Biosystems, Foster City, Calif., USA) as previously described [15,16]. The level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA was employed as an internal standard for calibration.…”

Section: Methodsmentioning

confidence: 99%

“…Splenic cells were isolated from 8-week-old BALB/c mice as previously described [16]. B cells, T cells and monocytes were isolated from splenic lymphocytes with the Auto-MACS™ column system (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) using a B Cell Isolation kit, Pan T Cell Isolation Kit and CD11b MicroBeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, Onishi et al [14] showed that oral administration of pulverized KGM prevented the elevation of plasma IgE, as well as the development of eczematous lesions and scratching behavior, in NC/Nga mice, a model for atopic dermatitis. In our previous studies [15,16], we also demonstrated that the administration of a pulverized KGM diet reduced the increase in plasma IgE, IgG1 and IgG2b, but not IgG2a, and the amount of ε-germline transcript (εGT) in the spleen induced by the injection of ovalbumin (OVA) and/or the injection of extract of syngeneic keratinocytes in BALB/c mice. However, the expression of mRNA for IFNγ was also suppressed in mice fed with the pulverized KGM diet.…”

Section: Introductionmentioning

confidence: 99%

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Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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Background: Oral administration of pulverized Konjac glucomannan (KGM) reduces increased plasma IgE and the amount of Ε-germline transcript (ΕGT) in the spleen, as well as preventing the development of dermatitis in mice. To elucidate the mechanism of action of pulverized KGM, we solubilized KGM and studied its effect on IgE in vitro and in vivo. Methods: Solubilized KGM was prepared by acid hydrolysis, and we analyzed the effective molecular size for the suppression of IgE production and ΕGT in vitro and the level of plasma IgE induced by immunization with ovalbumin in BALB/c mice. Results: The production of IgE and ΕGT in splenic cells, but not purified B cells, was inhibited by hydrolyzed KGM (KGM hydrolyzed with 0.25 N HCl; H-KGM) at the optimal size of between 10 and 500 kDa. However, no effect was observed when H-KGM was substituted with unhydrolyzed KGM in vitro. IgE production from purified B cells cocultured with purified monocytes, but not with purified T cells, was inhibited by H-KGM. The release of IFNγ in cultures of monocytes but in purified B cells with or without T cells was enhanced in the presence of H-KGM. Injection of mice with H-KGM also suppressed the production of plasma IgE and IgG1 but not IgG2a in vivo. Conclusion: KGM at an optimal size prevents germline class-switching and IgE production both in vitro and in vivo. H-KGM may be useful as a tool to study the mechanism of action of KGM and as a dietary supplement to prevent atopic diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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Contribution of itch-associated scratch behavior to the development of skin lesions in Dermatophagoides farinae-induced dermatitis model in NC/Nga mice

et al. 2008

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Recently, we have reported that the pathophysiological features of dermatitis induced by the repeated application with Dermatophagoides farinae (Df) extract ointment in NC/Nga mice were similar to those observed in the patients with atopic dermatitis. In the present study, we first examined whether the application of Df in other mouse strains could induce dermatitis. The repeated application of Df body (Dfb) ointment to the barrier-disrupted back of ICR, C57BL/6, and Balb/c mice did not cause any apparent skin lesions, although transient increase in serum immunoglobulin E (IgE) levels during antigen application was observed. On the other hand, in NC/Nga mice, dermatitis scores and serum IgE levels increased remarkably, and then these changes sustained for at least 10 days after stopping of antigen elicitation. Using NC/Nga mice, we investigated the contribution of scratching behavior to the development and maintenance of Dfb-induced dermatitis. In correlation with the increase in scratching behavior, erythema, hemorrhage, edema, scarring, erosion and excoriation were observed. Cutting off the hind toenails of mice exhibiting chronic skin lesions dramatically alleviated the dermatitis. From these findings, the onset of skin lesions and its chronically sustained course in Dfb-induced dermatitis in NC/Nga mice were closely associated with increased scratching behavior.

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