2006
DOI: 10.1007/s00403-005-0636-y
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The extract of syngeneic keratinocytes enhances IgE production from BALB/c mouse splenic lymphocytes in vitro

Abstract: The increase of serum IgE levels is closely associated with atopic dermatitis. We have previously revealed that cellular extract of PAM212 cells (PAM-extract), BALB/c mouse keratinocyte cell line, induced a remarkable increase of serum IgE levels, in vivo, when subcutaneously injected into BALB/c mice. However, precise mechanism of IgE-increasing activity was unclear. To elucidate the mechanism of IgE-increase in sera of BALB/c mice induced by PAM-extract, we explored the direct influence of PAM-extract on imm… Show more

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Cited by 3 publications
(3 citation statements)
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“…The levels of εGT in B cells, cultured as described above, were evaluated by quantitative real-time RT-PCR with an ABI PRISM 7700 sequence detector (Applied Biosystems, Foster City, Calif., USA) as previously described [15,16]. The level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA was employed as an internal standard for calibration.…”
Section: Methodsmentioning
confidence: 99%
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“…The levels of εGT in B cells, cultured as described above, were evaluated by quantitative real-time RT-PCR with an ABI PRISM 7700 sequence detector (Applied Biosystems, Foster City, Calif., USA) as previously described [15,16]. The level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA was employed as an internal standard for calibration.…”
Section: Methodsmentioning
confidence: 99%
“…Splenic cells were isolated from 8-week-old BALB/c mice as previously described [16]. B cells, T cells and monocytes were isolated from splenic lymphocytes with the Auto-MACS™ column system (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) using a B Cell Isolation kit, Pan T Cell Isolation Kit and CD11b MicroBeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) respectively.…”
Section: Methodsmentioning
confidence: 99%
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