Fucoidan prevents Cε germline transcription and NFκB p52 translocation for IgE production in B cells

Oomizu, Souichi; Yanase, Yuhki; Suzuki, Hidenori; Kameyoshi, Yoshikazu; Hide, Michihiro

doi:10.1016/j.bbrc.2006.08.009

Cited by 63 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have recently demonstrated that fucoidan, a polysaccharide isolated from sea weed, inhibits IgE production by both spleen cells and B cells purified from spleen in vitro [17]. This effect is mediated by the suppression of NFĸB p52, which is activated by the ligation of CD40 on B cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Oral administration of pulverized Konjac glucomannan (KGM) reduces increased plasma IgE and the amount of Ε-germline transcript (ΕGT) in the spleen, as well as preventing the development of dermatitis in mice. To elucidate the mechanism of action of pulverized KGM, we solubilized KGM and studied its effect on IgE in vitro and in vivo. Methods: Solubilized KGM was prepared by acid hydrolysis, and we analyzed the effective molecular size for the suppression of IgE production and ΕGT in vitro and the level of plasma IgE induced by immunization with ovalbumin in BALB/c mice. Results: The production of IgE and ΕGT in splenic cells, but not purified B cells, was inhibited by hydrolyzed KGM (KGM hydrolyzed with 0.25 N HCl; H-KGM) at the optimal size of between 10 and 500 kDa. However, no effect was observed when H-KGM was substituted with unhydrolyzed KGM in vitro. IgE production from purified B cells cocultured with purified monocytes, but not with purified T cells, was inhibited by H-KGM. The release of IFNγ in cultures of monocytes but in purified B cells with or without T cells was enhanced in the presence of H-KGM. Injection of mice with H-KGM also suppressed the production of plasma IgE and IgG1 but not IgG2a in vivo. Conclusion: KGM at an optimal size prevents germline class-switching and IgE production both in vitro and in vivo. H-KGM may be useful as a tool to study the mechanism of action of KGM and as a dietary supplement to prevent atopic diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Pulverized KGM was prepared by Shimizu Chemical (Mihara, Japan) as described before [14,17] and provided as a gift. …”

Section: Methodsmentioning

confidence: 99%

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that it protects bone marrow cells from radiation [1] and inhibits selectin-mediated adhesion of hematopoietic progenitor cells [2]. Also, fucoidan modulates the function of various immune cells such as lymphocytes [3], dendritic cells (DCs) [4], natural killer (NK) cells [5], and neutrophils [6].…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Enhances the Survival and Sustains the Number of Splenic Dendritic Cells in Mouse Endotoxemia

Ko¹,

Joo

2011

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including immunostimulation. In this study, we investigated whether fucoidan has beneficial effects on endotoxemia induced by LPS, a septic model in mice. The focus of this study was on survival rates and spleen function of the mice upon treatment. W e found that fucoidan had prophylactic effects on the survival rate of mice with endotoxemia. Flow cytometric analysis using antibodies for subset-specific markers revealed that fucoidan profoundly reversed the depleted population of dendritic cells in mice with endotoxemia. According to W estern blot analysis, the spleen cells of LPS/fucoidan-treated mice showed a higher expression of anti-apoptotic molecules compared to those of LPS-treated mice. Also, fucoidan-treated spleen cells were more responsive to mitogens. Taken together, these results demonstrate that fucoidan pre-treatment has beneficial effects on the survival rate and function of the spleen in mice with endotoxemia. This study may broaden the use of fucoidan in clinical fields, especially endotoxemia.

show abstract

“…The anti-inflammatory effects of fucoidan have been documented in a number of studies, and are related to the inhibition of the nuclear transcription factor NFjB, extracellular signal-regulated kinase (ERK), mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK) and Akt, as well as to the attenuation of proto-oncogene c-Fos or c-Jun transcriptional activity, which prevents the transactivation of activator protein 1 (AP-1) (Patel et al 2002;Oomizu et al 2006;Lee et al 2008Lee et al , 2011Du et al 2010;Do et al 2010). Li et al (2011) studied the mechanisms of the anti-inflammatory action of fucoidan in a rat myocardial ischemia-reperfusion model.…”

Section: Signaling Moleculesmentioning

confidence: 99%

Prospects for the therapeutic application of sulfated polysaccharides of brown algae in diseases of the cardiovascular system: review

Ts¹,

Беседнова²

2016

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Fucoidans are water-soluble, highly sulfated, branched homo-and hetero-polysaccharides derived from the fibrillar cell walls and intercellular spaces of brown seaweeds of the class Phaeophyceae. Fucoidans possess mimetic properties of the natural ligands of protein receptors and regulate functions of biological systems via key signaling molecules. Objectives: The aim of this review was to collect and combine all available scientific literature about the potential use of the fucoidans for diseases of cardiovascular system. Materials and methods: The review has been compiled using references from major databases such as Web of Science, PubMed, Scopus, Elsevier, Springer and Google Scholar (up to September 2015). After obtaining all reports from database (a total number is about 580), the papers were carefully analyzed in order to find data related to the topic of this review (129 references). Results: An exhaustive survey of literature revealed that fucoidans possess a broad spectrum of biological activity, including anti-coagulant, hypolipidemic, anti-thrombotic, anti-inflammatory, immunomodulatory, anti-tumor, anti-adhesive and anti-hypertensive properties. Numerous investigations of fucoidans in diseases of the cardiovascular system mainly focus on pleiotropic anti-inflammatory effects. Fucoidans also possess pro-angiogenic and pro-vasculogenic properties. Conclusion: A great number of investigations in the past years have demonstrated that fucoidans has great potential for in-depth investigation of their effects on cardiovascular system. Through this review, the authors hope to attract the attention of researchers to use fucoidan as mimetic of natural ligand receptor protein with the view of developing new formulations with an improved therapeutic value. ARTICLE HISTORY

show abstract

Fucoidan prevents Cε germline transcription and NFκB p52 translocation for IgE production in B cells

Cited by 63 publications

References 33 publications

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Fucoidan Enhances the Survival and Sustains the Number of Splenic Dendritic Cells in Mouse Endotoxemia

Prospects for the therapeutic application of sulfated polysaccharides of brown algae in diseases of the cardiovascular system: review

Contact Info

Product

Resources

About