The Extracellular Matrix of the Liver

Rojkind, Marcos; Ponce-Noyola, Patricia

doi:10.1016/s0174-173x(82)80031-9

Cited by 158 publications

(84 citation statements)

References 114 publications

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“…The proportions of the collagen species in these cultures parallels those seen in adult rat liver. 12 The present study also shows that cells derived from mature hepatocytes can interact to develop many organotypic structures occurring in three dimensions during liver development, such as ducts, plates, and spaces between plates reminiscent of sinusoidal areas. The cells in these cultures are of two types, epithelial and mesenchymal.…”

Section: Discussionsupporting

confidence: 65%

Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor

et al. 1999

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Section: Discussionsupporting

confidence: 65%

Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor

et al. 1999

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“…However, there is disagreement about whether hepatocytes express type I collagen in normal liver. 39 On the other hand, LECs and stellate cells are reported to produce the type IV collagen and laminin, 33 suggesting that the major source of the former is stellate cells, while the latter is derived predominantly from stellate and SECs. Activated stellate cells are also the major source of the interstitial collegens (types I and III), which are produced in excess on collagen-coated dishes.…”

Section: Effects Of Npcs and Ecm On The Differentiation Of Shsmentioning

confidence: 99%

Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells

et al. 1999

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Landry et al. 1 first showed the reconstruction of a threedimensional cyto-architecture consisting of differentiated hepatocytes, bile duct-like cells, and deposited extracellular matrix (ECM) by the use of spheroidal aggregate culture of hepatic cells isolated from newborn rats. Thereafter, some attempts have been made to grow a hepatic organoid by the coculture of hepatocytes and fibroblasts. 2,3 As neither fibroblasts derived from the liver nor hepatocytes could proliferate, the size of the cell aggregates was limited and the position of the cells was disorderly, although differentiated functions were well maintained for a long time. To develop a hepatic organoid, the interactions not only between hepatocytes and nonparenchymal cells (NPCs) but also between ECM and hepatocytes must be well coordinated as many differentiated functions of mature hepatocytes (MHs), through which the homeostasis of life is sustained, should be maintained.We have reported that the proliferation of adult rat hepatocytes is observed in serum-free medium supplemented with 10 mmol/L nicotinamide and epidermal growth factor (EGF). [4][5][6][7][8] Other laboratories also reported that supplementation of the medium with nicotinamide and EGF stimulates the growth of the primary rat hepatocytes. 9,10 Most hepatocytes cultured in nicotinamide-supplemented medium can divide, and small hepatocytes (SHs), which are less than half as large as but are morphologically similar to MHs, proliferate so fast that the cells form colonies after 4 to 5 days of culture. They have been shown immunocytochemically and ultrastructurally to possess hepatic characteristics. 6 Recently, it was shown by our 11 and other laboratories 12 that a single SH can clonally proliferate and form a large colony consisting of more than 30 cells at day 10. The cells can grow and survive for more than 5 months. Thus, we previously proposed that rat hepatocytes may be classified into three types of cells with respect to their ability to divide: (1) cells that have a high potential to proliferate and form colonies in primary culture (type I cells; SHs), (2) cells for which the number of possible cell divisions is limited (type II cells), and (3) cells that lose the ability to divide (type III cells). 7,8 In addition, type II and type III cells may possess fully differentiated functions. Therefore, in this report we call both type II and type III cells MHs. Furthermore, we call the cell islands, which consist of SHs themselves and of a mixture of SHs and MHs, colonies.In the present experiment we showed that SHs could differentiate to MHs that interacted with hepatic NPCs and

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“…0.5% of total protein in normal liver consists of collagen, types I, Ill, IV, and V collagen ( 11), but CCl4-induced rat liver fibrosis increases this to 2% of total protein (12). Interstitial collagen consisting of types I and III accounts for nearly 90-95% of all collagen found in the normal liver ( 11). The ratio of types I and III collagen, 60 and 40% of total liver collagen, respectively, did not change upon CCl4-induced rat liver fibrosis (12).…”

Section: Introductionmentioning

confidence: 99%

Coexpression of the collagen-binding stress protein HSP47 gene and the alpha 1(I) and alpha 1(III) collagen genes in carbon tetrachloride-induced rat liver fibrosis.

Fukumoto²,

et al. 1994

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HSP47 is a collagen-binding stress protein and is assumed to act as a collagen-specific molecular chaperone during the biosynthesis and secretion of procollagen in the living cell. The synthesis of HSP47 has been reported to correlate with that of collagen in several cell lines.We examined the expression of HSP47 mRNA during the progression of carbon tetrachloride (CC14)-induced liver fibrosis in rats. Northern blot analysis revealed that the expression of HSP47 mRNA was markedly induced during the progression of fibrosis in parallel with al (I) and al (III) collagen mRNAs. By in situ hybridization, the distribution of HSP47 transcripts was similar to that of al (I) collagen and was observed only in cells lining collagen fibrils. These collagen-positive cells were confirmed to be Ito cells by immunohistochemistry for desmin. The absence of high levels of HSP47 mRNA in the liver of rats treated with only a

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The Extracellular Matrix of the Liver

Cited by 158 publications

References 114 publications

Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor

Morphogenetic events in mixed cultures of rat hepatocytes and nonparenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor

Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells

Coexpression of the collagen-binding stress protein HSP47 gene and the alpha 1(I) and alpha 1(III) collagen genes in carbon tetrachloride-induced rat liver fibrosis.

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