The extracellular matrix and Ca(2+)signaling mechanisms

Abstract

The extracellular matrix (ECM) consists of proteins, glycosaminoglycans and glycoproteins, that support the dynamic interactions between cells, including intercellular communication, cell attachment, cell differentiation, cell growth and migration. As such, the ECM represents an essential and very sensitive system within the tissue microenvironment that is involved in processes such as tissue regeneration and carcinogenesis. The aim of the present review is to evaluate its diversity through Ca 2+ signaling and its role in muscle cell function. Here, we discuss some methodological approaches dissecting Ca 2+ handling mechanisms in myogenic and non-myogenic cells, e.g. the importance of Ca 2+ and calpains in muscle dystrophy. We also consider the reconstruction of skeletal muscle by colonization of decellularized ECM with muscle-derived cells isolated from skeletal muscle. Therefore, it is necessary to establish new methodological procedures based on Ca 2+ signaling in skeletal muscle cells and their effect on ECM homeostasis, allowing the monitoring of skeletal muscle reconstruction and organ repair.

Global transcriptomic analysis reveals Lnc-ADAMTS9 exerting an essential role in myogenesis through modulating the ERK signaling pathway

“…Ca 2+ is necessary for cell proliferation and G 1 is the most sensitive phase of the cell cycle to Ca 2+ depletion [34]. Intracellular Ca 2+ concentrations and calcium signaling pathways are important factors in regulating cell proliferation and differentiation [35,36]. Intracellular Ca 2+ activates the MAPK pathway and resultantly participates in stem cell differentiation into lineages [37,38].…”