The extracellular calcium-sensing receptor (CaSR) on human esophagus and evidence of expression of the CaSR on the esophageal epithelial cell line (HET-1A)

Justinich, Christopher J.; Mak, Nanette; Pacheco, Ivan; Mulder, Daniel J.; Wells, Ron W.; Blennerhassett, Michael G.; Macleod, John

doi:10.1152/ajpgi.00226.2006

Cited by 37 publications

(31 citation statements)

References 53 publications

(47 reference statements)

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“…In the study by Cheng et al (2004), it was reported that polyamine-induced effects required the presence of Ca (Canaff et al, 2001), suggestive of being an orthosteric agonist. In addition to their actions in the colon, polyamines have also been described to produce Ca 2ϩ i mobilization and ERK1/2 activation using the human esophageal epithelial cell line (HET-1A) and in basal cells of the human esophagus, through the activation of the CaR (Justinich et al, 2008). Moreover, in G cells of the stomach, which express the CaR, spermine was shown to stimulate gastrin release in a concentration-dependent manner (Ray et al, 1997).…”

Section: ؉ Omentioning

confidence: 99%

Agonists and Allosteric Modulators of the Calcium-Sensing Receptor and Their Therapeutic Applications

Saidak¹,

Brazier²,

Kamel³

et al. 2009

Mol Pharmacol

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Section: ؉ Omentioning

confidence: 99%

Agonists and Allosteric Modulators of the Calcium-Sensing Receptor and Their Therapeutic Applications

Saidak¹,

Brazier²,

Kamel³

et al. 2009

Mol Pharmacol

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“…However, since the identi�ication and characterisation of the receptor [2], it has become increasingly apparent that this cationic ion binding receptor is found on many tissues not associated with the control of plasma [Ca 2+ ] including; oesophageal [3] and colonic epithelia [4], the cardiovascular system (reviewed in [5]), hypothalamic neurons [6], pancreatic ducts [7] and pancreatic α-and β-cells [8][9][10]. The functional signi�icance of the CaR in tissue not involved in the control of systemic Ca 2+ is not fully understood [11,12].…”

Section: Introductionmentioning

confidence: 99%

Calcium-Sensing Receptor Activation Increases Cell-Cell Adhesion and ß-Cell Function

Hills¹,

Younis²,

Bennett³

et al. 2012

Cell Physiol Biochem

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Background/Aims: The extracellular calcium-sensing receptor (CaR) is expressed in pancreatic β-cells where it is thought to facilitate cell-to-cell communication and augment insulin secretion. However, it is unknown how CaR activation improves β-cell function. Methods: Immunocytochemistry and western blotting confirmed the expression of CaR in MIN6 β-cell line. The calcimimetic R568 (1µM) was used to increase the affinity of the CaR and specifically activate the receptor at a physiologically appropriate extracellular calcium concentration. Incorporation of 5-bromo-2’-deoxyuridine (BrdU) was used to measure cell proliferation, whilst changes in non-nutrient-evoked cytosolic calcium were assessed using fura-2-microfluorimetry. AFM-single-cell-force spectroscopy related CaR-evoked changes in epithelial (E)-cadherin expression to improved functional tethering between coupled cells. Results: Activation of the CaR over 48hr doubled the expression of E-cadherin (206±41%) and increased L-type voltage-dependent calcium channel expression by 70% compared to control. These changes produced a 30% increase in cell-cell tethering and elevated the basal-to-peak amplitude of ATP (50µM) and tolbutamide (100µM)-evoked changes in cytosolic calcium. Activation of the receptor also increased PD98059 (1-100µM) and SU1498 (1-100µM)-dependent β-cell proliferation. Conclusion: Our data suggest that activation of the CaR increases E-cadherin mediated functional tethering between β-cells and increases expression of L-type VDCC and secretagogue-evoked changes in [Ca²⁺]_i. These findings could explain how local changes in calcium, co-released with insulin, activate the CaR on neighbouring cells to help ensure efficient and appropriate secretory function.

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“…Recent data show that taste buds contain the CaSR and that agonists of the CaSR enhance taste perception while antagonists of the CaSR suppress it (Ohsu et al, 2010). The receptor is also expressed in the basal cells of the oesophagus where stimulation results in an increase in IL-8 secretion, the physiological importance of which is yet to be determined (Justinich et al, 2008). In the stomach, the CaSR plays a clear role in the regulation of gastrin and gastric acid release from G cells and parietal cells, respectively (Conigrave and Brown, 2006;Geibel and Hebert, 2009).…”

Section: +mentioning

confidence: 99%

CASR (Calcium-Sensing Receptor)

Ward¹,

Magno²,

Ratajczak³

2014

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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The extracellular calcium-sensing receptor (CaSR) on human esophagus and evidence of expression of the CaSR on the esophageal epithelial cell line (HET-1A)

Cited by 37 publications

References 53 publications

Agonists and Allosteric Modulators of the Calcium-Sensing Receptor and Their Therapeutic Applications

Agonists and Allosteric Modulators of the Calcium-Sensing Receptor and Their Therapeutic Applications

Calcium-Sensing Receptor Activation Increases Cell-Cell Adhesion and ß-Cell Function

CASR (Calcium-Sensing Receptor)

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