The extended production of UV-induced reactive oxygen species in L929 fibroblasts is attenuated by posttreatment with Arrabidaea chica through scavenging mechanisms

Ribeiro, Fabianne Martins; Volpato, Hélito; Lazarin-Bidóia, Danielle; Desoti, Vânia Cristina; Souza, Rebeca Oliveira de; Fonseca, Maria José Vieira; Ueda-Nakamura, Tânia; Silva, Sueli de Oliveira

doi:10.1016/j.jphotobiol.2017.11.002

Cited by 24 publications

(18 citation statements)

References 42 publications

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“…The purpose of fibroblast study was to investigate release behavior of PPADT nanoparticles in an environment with biologically relevant concentrations of ROS. Several methods have been used to challenge skin cells to produce increased levels of ROS, such as UV light, low pH, addition of serum amyloid A (5–15 μg/mL), IR irradiation, and addition of ROS species. − Silverberg and co-workers used different concentrations of H 2 O 2 to produce increased levels of ROS in fibroblasts, which was initially attempted here . Surprisingly, we found that changing the tonicity of the growth medium also challenged the cells to produce increased levels of ROS.…”

Section: Discussionmentioning

confidence: 99%

Reactive Oxygen Species-Responsive Polymer Nanoparticles to Improve the Treatment of Inflammatory Skin Diseases

et al. 2022

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To improve the quality of life for people living with chronic inflammatory skin diseases, we propose a new treatment strategy by exploring a stimuli-responsive drug delivery system. Formulations designed by exploiting smart materials can be programmed to perform a specific action upon exposure to disease-related stimuli. For instance, increased levels of reactive oxygen species (ROS), especially the accumulation of hydrogen peroxide, can be utilized to differentiate between healthy and inflamed tissues. In this concept-proofing study, the polymer poly(1,4 phenyleneacetone dimethylene thioketal) (PPADT) was investigated for its ROS-responsive properties and potential to treat inflammatory skin diseases. PPADT nanoparticles were formulated by oil-in-water emulsification followed by solvent evaporation and characterized by size, zeta-potential, and release kinetic profiles. Release profiles revealed that the PPADT nanoparticles were sensitive toward elevated levels of ROS in an ROS-stimulus concentration (0.1–10 mM) and time-dependent manner (flare-up mimicked). The safety assessment proved that the PPADT polymer and the monomers generated by oxidation do not show any sign of being cytotoxic to fibroblasts and no mutagenic liabilities were observed. In conclusion, the PPADT polymer demonstrated to be a promising material for stimuli-responsive delivery of hydrophobic small molecules in the treatment of inflammatory skin diseases.

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Section: Discussionmentioning

confidence: 99%

Reactive Oxygen Species-Responsive Polymer Nanoparticles to Improve the Treatment of Inflammatory Skin Diseases

et al. 2022

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“…Hydrogen peroxide (H 2 O 2 ) was used to establish an intracellular oxidative stress model [ 35 ]. ROS were analyzed using a CM-H 2 DCFDA molecular probe (Invitrogen, C.A., U.S.A.), which was measured on the basis of fluorescence because of the conversion of fluorescent DCF in the presence of ROS as nonfluorescent H 2 DCFDA dye readily penetrated in the cells [ 36 ], which is a commonly used system for directly evaluating cellular redox states, and is a typical indicator of oxidative stress [ 37 , 38 ]. The L-929 cells were seeded on the GO, GO/RGD, GO/AST, and GO/RGD/AST films.…”

Section: Methodsmentioning

confidence: 99%

Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

2022

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Background Astaxanthin (AST) is known as a powerful antioxidant that affects the removal of active oxygen and inhibits the production of lipid peroxide caused by ultraviolet light. However, it is easily decomposed by heat or light during production and storage because of the unsaturated compound nature with a structural double bond. The activity of AST can be reduced and lose its antioxidant capability. Graphene oxide (GO) is an ultrathin nanomaterial produced by oxidizing layered graphite. The chemical combination of AST with GO can improve the dispersion properties to maintain structural stability and antioxidant activity because of the tightly bonded functionalized GO surface. Methods Layered GO films were used as nanocarriers for the AST molecule, which was produced via flow-enabled self-assembly and subsequent controlled solution deposition of RGD peptide and AST molecules. Synthesis of the GO-AST complex was also carried out for the optimized concentration. The characterization of prepared materials was analyzed through transmission electron microscopy (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscope (AFM), and Raman spectroscopy. Antioxidant activity was tested by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2.2-diphenyl-1-picrylhydrazyl (DPPH) assays. The antibacterial effect and antioxidant effects were monitored for the ultrathin GO/RGD/AST Film. Further, reactive oxygen species (ROS) assay was used to evaluate the anti-inflammatory effects on L-929 fibroblasts. Results Cotreatment of GO-AST solution demonstrated a high antioxidant combined effect with a high ABTS and DPPH radicals scavenging activity. The GO/RGD/AST film was produced by the self-assembly process exhibited excellent antibacterial effects based on physicochemical damage against E. coli and S. aureus. In addition, the GO/RGD/AST film inhibited H2O2-induced intracellular ROS, suppressed the toxicity of lipopolysaccharide (LPS)-induced cells, and restored it, thereby exhibiting strong antioxidant and anti-inflammatory effects. Conclusion As GO nanocarrier-assisted AST exerted promising antioxidant and antibacterial reactions, presented a new concept to expand basic research into the field of tissue engineering.

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“…The use of several methodologies to evaluate the effects of F. chica extracts for the control of oxidative stress allowed for a more complete view of this mechanism. Other authors, such as Torres et al in 2018 [ 138 ] and Teixeira et al in 2017 [ 171 ], have included other free radical neutralization assays, such as ABTS (2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric reducing antioxidant power), thus reaffirming the biological effects of F. chica [ 138 , 169 , 170 , 171 ].…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

“…Martins et al, in 2016 [ 169 ] and Ribeiro et al, working in 2018 [ 170 ], demonstrated the antioxidant capacity of a hydroethanolic extract of Fridericia chica in the presence of both the free radical DPPH and the oxidative damage induced by ultraviolet radiation. The use of several methodologies to evaluate the effects of F. chica extracts for the control of oxidative stress allowed for a more complete view of this mechanism.…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Therapeutic Potential of Leaves from Fridericia chica (Bonpl.) L. G. Lohmann: Botanical Aspects, Phytochemical and Biological, Anti-Inflammatory, Antioxidant and Healing Action

et al. 2022

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Plants of the species Fridericia chica (Bonpl.) L. G. Lohmann (Bignoniaceae), which are widely distributed in Brazil and named crajiru in the state of Amazonas, are known in folk medicine as a traditional medicine in the form of a tea for the treatment of intestinal colic, diarrhea, and anemia, among other diseases. The chemical analysis of extracts of the leaves has identified phenolic compounds, a class of secondary metabolites that provide defense for plants and benefits to the health of humans. Several studies have shown the therapeutic efficacy of F. chica extracts, with antitumor, antiviral, wound healing, anti-inflammatory, and antioxidant activities being among the therapeutic applications already proven. The healing action of F. chica leaf extract has been demonstrated in several experimental models, and shows the ability to favor the proliferation of fibroblasts, which is essential for tissue repair. The anti-inflammatory activity of F. chica has been clearly demonstrated by several authors, who suggest that it is related to the presence of 3-deoxyanthocyanidins, which is capable of inhibiting pro-inflammatory pathways such as the kappa B (NF-kB) nuclear transcription factor pathway. Another important effect attributed to this species is the antioxidant effect, attributed to phenolic compounds interrupting chain reactions caused by free radicals and donating hydrogen atoms or electrons. In conclusion, the species Fridericia chica has great therapeutic potential, which is detailed in this paper with the objective of encouraging new research and promoting the sum of efforts for the inclusion of herbal medicines in health systems around the world.

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The extended production of UV-induced reactive oxygen species in L929 fibroblasts is attenuated by posttreatment with Arrabidaea chica through scavenging mechanisms

Cited by 24 publications

References 42 publications

Reactive Oxygen Species-Responsive Polymer Nanoparticles to Improve the Treatment of Inflammatory Skin Diseases

Reactive Oxygen Species-Responsive Polymer Nanoparticles to Improve the Treatment of Inflammatory Skin Diseases

Surface-mediated high antioxidant and anti-inflammatory effects of astaxanthin-loaded ultrathin graphene oxide film that inhibits the overproduction of intracellular reactive oxygen species

Therapeutic Potential of Leaves from Fridericia chica (Bonpl.) L. G. Lohmann: Botanical Aspects, Phytochemical and Biological, Anti-Inflammatory, Antioxidant and Healing Action

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