The expression profile of microRNAs in a model of 7,12-dimethyl-benz[a]anthrance-induced oral carcinogenesis in Syrian hamster

Abstract

Background: Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been done to compare the microRNA expression patterns in oral cancer. In this study, we constructed an animal model of oral squamous cell carcinoma to investigate expression profiles of microRNAs in oral carcinogenesis.

MicroRNAs miR-199a-5p and -3p Target the Brm Subunit of SWI/SNF to Generate a Double-Negative Feedback Loop in a Variety of Human Cancers

“…36). In comparison with cells from a normal epithelium, in situ hybridization analysis revealed marginal levels of miR-199a-5p and -3p expression in invasive areas, consistent with a previous report (25), and even in carcinoma in situ (Supplementary Table S3). By immunostaining, we also observed lower levels of Egr1 in invasive areas; Brm expression was shown to be equivalent or higher to that in a normal epithelium.…”

“…Among these, 2 candidates with high scores were predicated by PicTar, miR199a-5p, and miR-199a-3p. Both of these miRNAs are produced from a single molecule, pre-miR-199a, and have been reported to be upregulated in cervical (22) and ovarian (23) cancers but downregulated in liver (24) and oral (25) cancers. We thus focused on these candidates in our analyses and evaluated whether they truly target Brm mRNA via the predicted binding sites in the 3 0 -untranslated region (UTR; Fig.…”

Effects of targeted modulation of miR-762 on expression of the IFITM5 gene in Saos-2 cells

“…MiR-762 is reportedly involved in tumorigenesis and in the development and progression of diseases such as diabetes and immune system diseases; miR-762 acts by targeting a number of important genes (21)(22)(23). Significance analysis of microarrays initially revealed that miR-762 is significantly upregulated in oral squamous cell carcinoma, suggesting that miR-762 plays a role in oral carcinogenesis (21).…”

“…Significance analysis of microarrays initially revealed that miR-762 is significantly upregulated in oral squamous cell carcinoma, suggesting that miR-762 plays a role in oral carcinogenesis (21). Recently, the neural precursor cell-enriched miR-762 was found to translationally downregulate adenosyl methionine decarboxylase 1 (Amd1), a key enzyme required for the synthesis of the polyamines spermine and spermidine, as it regulates both embryonic stem cell self-renewal and differentiation into a neural lineage (24).…”

microRNA-143, down-regulated in osteosarcoma, promotes apoptosis and suppresses tumorigenicity by targeting Bcl-2

“…Recently, miR-143 has been reported to be downregulated in some types of cancer, such as colorectal cancer (9)(10)(11)(12)(13), prostate cancer (14,15), oral squamous cell carcinoma (16), pituitary tumor (17), cervical cancer (18), nasopharyngeal carcinoma (19) and lymphoma (20,21). In these malignancies, forced expression of miR-143 inhibits cancer cell growth.…”