The expression of type II TA system genes following exposure to the sub-inhibitory concentration of gentamicin and acid stress in Brucella spp.

Amraei, Fatemeh; Narimisa, Negar; Kalani, Behrooz Sadeghi; Mohammadzadeh, Rokhsareh; Lohrasbi, Vahid; Jazi, Faramarz Masjedian

doi:10.1016/j.micpath.2020.104194

Cited by 8 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following reports from Amraei et al . (2020) that B. abortus (B19) and B. melitensis (16M) display biphasic killing curves in broth in presence of ampicillin and gentamicin [62, 63], we tested whether this could also be observed for ciprofloxacin treatment of B. abortus .…”

Section: Resultsmentioning

confidence: 99%

“…display biphasic killing curves in broth in presence of ampicillin and gentamicin [62,63], we tested whether this could also be observed for ciprofloxacin treatment of B. abortus.…”

Section: Following Reports From Amraei Et Al (2020) That B Abortus (B...mentioning

confidence: 99%

“…It is patent that Brucella ’s ability to invade and replicate intracellularly constitutes a hurdle for the successful treatment of Brucellosis, as for its ability to disperse in and colonize a broad variety of niches within its host. Moreover, the possibility that Brucella exhibits a “persister” phenotype during infection could, as recently suggested in vitro [62, 63], constitute an additional complexity layer towards successful therapy. Indeed, so-called persisters constitute a challenge for clinical microbiology due to its multidrug-tolerant manifestation [64–71].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antibiotic persistence of Brucella abortus in its protective intracellular niche

Mode

Ketterer

Québatte

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Human Brucellosis caused by the facultative intracellular pathogen Brucella spp. is an endemic bacterial zoonosis manifesting as acute or chronic infections associated with high morbidity. Treatment typically involves a combination therapy of two antibiotics administered for several weeks to month, but despite this harsh treatment relapses occur at a rate of 5-15%. Although poor compliance and reinfection may account for a fraction of the observed relapse cases, it is apparent that the properties of the infectious agent itself may play a decisive role in this phenomenon. Methodology/Principal findings: We used B. abortus carrying a dual reporter in a macrophage infection model to gain a better understanding of the efficacy of recommended therapies in cellulo. For this we used automated fluorescent microscopy as a prime read-out and developed specific CellProfiler pipelines to score infected macrophages at the population and the single cell level. Combining microscopy of constitutive and induced reporters with classical CFU determination, we quantified the protective nature of the Brucella intracellular niche to various antibiotics and the ability of B. abortus to persist in cellulo despite harsh antibiotic treatments. Conclusion/Significance: We demonstrate that treatment of infected macrophages with antibiotics at recommended concentrations fails to fully prevent growth and persistence of B. abortus in cellulo, which may be explained by a protective nature of the intracellular niche. Moreover, we show the presence of bona fide intracellular persisters upon antibiotic treatment, which are metabolically active and retain the full infectious potential, therefore constituting a plausible reservoir for reinfection and relapse. In conclusion, our results highlight the need to extend the spectrum of models to test new antimicrobial therapies for Brucellosis to better reflect the in vivo infection environment, and to develop therapeutic approaches targeting the persister subpopulation.

show abstract

Section: Resultsmentioning

confidence: 99%

“…display biphasic killing curves in broth in presence of ampicillin and gentamicin [62,63], we tested whether this could also be observed for ciprofloxacin treatment of B. abortus.…”

Section: Following Reports From Amraei Et Al (2020) That B Abortus (B...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic persistence of Brucella abortus in its protective intracellular niche

Mode

Ketterer

Québatte

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Expression of the TA systems relE/RHH-like, fic/phd and brnTA increased following exposure of Brucella spp. strains to acid shock conditions (pH 5.5), suggesting a possible role for these TA systems in acidic conditions [148]. PemIK has also been shown to influence biofilm formation in the Klebsiella pneumoniae strain ST846-OXA48CA, as it was found on a plasmid within this strain that also shows tolerance to chlorohexidine treatment [167].…”

Section: Other Stressesmentioning

confidence: 98%

“…Deletion of mqsRA [145,146] or yafQ [147] has been shown to reduce the survival of E. coli under antibiotic exposure, suggesting that these TA loci contribute to bacterial persistence. Other examples include the TA systems RelE/RHH-like and BrnTA in Brucella spp., with the expression of these genes increasing following exposure to sub-MIC concentrations of gentamicin [148]. Chromosomal mazEF in Staphyloccocus aureus is also thought to play a role in β-lactam resistance through regulation of other genes, with mazEF deletion mutants exhibiting increased susceptibility to penicillin [149].…”

Section: Antibiotic Tolerance and Persister Formationmentioning

confidence: 99%

Biological Functions of Type II Toxin-Antitoxin Systems in Bacteria

Kamruzzaman

Iredell

2021

Microorganisms

View full text Add to dashboard Cite

After the first discovery in the 1980s in F-plasmids as a plasmid maintenance system, a myriad of toxin-antitoxin (TA) systems has been identified in bacterial chromosomes and mobile genetic elements (MGEs), including plasmids and bacteriophages. TA systems are small genetic modules that encode a toxin and its antidote and can be divided into seven types based on the nature of the antitoxin molecules and their mechanism of action to neutralise toxins. Among them, type II TA systems are widely distributed in chromosomes and plasmids and the best studied so far. Maintaining genetic material may be the major function of type II TA systems associated with MGEs, but the chromosomal TA systems contribute largely to functions associated with bacterial physiology, including the management of different stresses, virulence and pathogenesis. Due to growing interest in TA research, extensive work has been conducted in recent decades to better understand the physiological roles of these chromosomally encoded modules. However, there are still controversies about some of the functions associated with different TA systems. This review will discuss the most current findings and the bona fide functions of bacterial type II TA systems.

show abstract

Prevalence of Brucella endocarditis: A systematic review and meta‐analysis

Narimisa

Razavi

Khoshbayan

et al. 2023

Health Science Reports

Self Cite

View full text Add to dashboard Cite

Background Endocarditis caused by Brucella infection is one of this infection's complications, including a high mortality rate. However, studies on the prevalence of this complication have been limited to some case reports. This study investigated the prevalence of Brucella endocarditis globally using a systematic review and meta‐analysis. Methods PubMed, Scopus, and Web of Science databases were searched using appropriate keywords until September 2022. All studies reporting the prevalence of endocarditis in patients with brucellosis were included in this current study. To investigate the pooled prevalence of Brucella endocarditis, random model was used in comprehensive meta‐analysis software. Results A total of 25 studies met the inclusion criteria and were included in the systematic review and meta‐analysis. The prevalence of Brucella endocarditis was 1.3%, and the death rate was 26.5%. The results did not show a significant difference in the prevalence of this complication in different regions. Conclusion According to this study's results, the prevalence of Brucella endocarditis is low, but it includes a large percentage of the deaths of affected patients. To complete our understanding of this complication and its management, more research should be done to investigate the effect of other factors, such as age and gender.

show abstract

The expression of type II TA system genes following exposure to the sub-inhibitory concentration of gentamicin and acid stress in Brucella spp.

Cited by 8 publications

References 20 publications

Antibiotic persistence of Brucella abortus in its protective intracellular niche

Antibiotic persistence of Brucella abortus in its protective intracellular niche

Biological Functions of Type II Toxin-Antitoxin Systems in Bacteria

Prevalence of Brucella endocarditis: A systematic review and meta‐analysis

Contact Info

Product

Resources

About