The expression of two collagen receptor subfamilies, integrins and discoidin domains during osteogenic and chondrogenic differentiation of human mesenehymal stem cells

Xue, Feiqun; Zhou, Wei; Ze-dong, Lan

doi:10.3233/bme-201151

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…The expression of DDR2 and integrin α11β1 was increased when human bone marrow MSC were cultured on a substrate with type 1 collagen. It was found that the expression of integrin α11β1 prevailed during chondrogenic differentiation, while the expression of DDR2 was also significantly higher during osteogenic differentiation [ 84 ].…”

Section: Ecm Receptor Signaling Pathways During Msc Differentiationmentioning

confidence: 99%

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Novoseletskaya,

Evdokimov,

Efimenko

2023

Cell Commun Signal

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The extracellular matrix (ECM) is a crucial component of the stem cell microenvironment, or stem-cell niches, and contributes to the regulation of cell behavior and fate. Accumulating evidence indicates that different types of stem cells possess a large variety of molecules responsible for interactions with the ECM, mediating specific epigenetic rearrangements and corresponding changes in transcriptome profile. Signals from the ECM are crucial at all stages of ontogenesis, including embryonic and postnatal development, as well as tissue renewal and repair. The ECM could regulate stem cell transition from a quiescent state to readiness to perceive the signals of differentiation induction (competence) and the transition between different stages of differentiation (commitment). Currently, to unveil the complex networks of cellular signaling from the ECM, multiple approaches including screening methods, the analysis of the cell matrixome, and the creation of predictive networks of protein–protein interactions based on experimental data are used. In this review, we consider the existing evidence regarded the contribution of ECM-induced intracellular signaling pathways into the regulation of stem cell differentiation focusing on mesenchymal stem/stromal cells (MSCs) as well-studied type of postnatal stem cells totally depended on signals from ECM. Furthermore, we propose a system biology-based approach for the prediction of ECM-mediated signal transduction pathways in target cells.

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Section: Ecm Receptor Signaling Pathways During Msc Differentiationmentioning

confidence: 99%

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Novoseletskaya,

Evdokimov,

Efimenko

2023

Cell Commun Signal

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“…There are at least 18 α subunits and 8 β subunits in humans, which together generate 24 integrin proteins. Among them, integrin β1 (ITGB1) is the hub subunit, which can combine with other 12 α subunits like α2β1 and α11β1 integrin [ 20 ], and it belongs to the integrin family mainly associated with chondrogenesis [ 21 , 22 ]. The integrin β binds to the domain of TGF-β precursor to activate TGF-β precursor to eventually release the mature TGF-β growth factor that contributes to chondrogenesis [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel

Huang,

Sun,

et al. 2024

Regenerative Biomaterials

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Cartilage defects may lead to severe degenerative joint diseases. Tissue engineering based on type I collagen hydrogel that has chondrogenic potential is ideal for cartilage repair. However, the underlying mechanisms of chondrogenic differentiation driven by type I collagen hydrogel have not been fully clarified. Herein, we explored potential collagen receptors and chondrogenic signaling pathways through bioinformatical analysis to investigate the mechanism of collagen-induced chondrogenesis. Results showed that the super enhancer-related genes induced by collagen hydrogel were significantly enriched in the TGF-ß signaling pathway, and integrin-ß1 (ITGB1), a receptor of collagen, was highly expressed in bone marrow mesenchymal stem cells (BMSCs). Further analysis showed genes such as COL2A1 and Tenascin C (TNC) that interacted with ITGB1 were significantly enriched in extracellular matrix (ECM) structural constituents in the chondrogenic induction group. Knockdown of ITGB1 leaded to the down-regulation of cartilage-specific genes (SOX9, ACAN, COL2A1), SMAD2, and TNC, as well as the down-regulation of phosphorylation of SMAD2/3. Knockdown of TNC also resulted in decrease of cartilage markers, ITGB1 and the SMAD2/3 phosphorylation but overexpression of TNC showed the opposite trends. Finally, in vitro and in vivo experiments confirmed the involvement of ITGB1 and TNC in collagen-mediated chondrogenic differentiation and cartilage regeneration. In summary, we demonstrated that ITGB1 was a crucial receptor for chondrogenic differentiation of BMSCs induced by collagen hydrogel. It can activate TGF-SMAD2/3 signaling, followed by impacting TNC expression, which in turn promotes the interaction of ITGB1 and TGF-SMAD2/3 signaling to enhance chondrogenesis. These may provide concernful supports for cartilage tissue engineering and biomaterials development.

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The expression of two collagen receptor subfamilies, integrins and discoidin domains during osteogenic and chondrogenic differentiation of human mesenehymal stem cells

Cited by 2 publications

References 34 publications

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Extracellular matrix-induced signaling pathways in mesenchymal stem/stromal cells

Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel

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