The expression of the env gene-related gp66 in mouse cells infected with the helper independent friend leukemia virus is restricted to the myelomonocytic and mastocytic lineages

Mathieu-Mahul, D.; Heard, Jean‐Michel; Fichelson, Serge; Mauchauffé, M; Larsen, Christian‐Jacques

doi:10.1016/0042-6822(84)90036-9

Cited by 2 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From this finding, we speculated that p115 might be a transit form of IL-2R on a pathway of ever-recycling, or ever-replacing of this receptor (43). However, the possibility that p115 denotes a "fusion protein" or "polyprotein" containing a primitive precursor for IL-2R (such as an insulin receptor (4) or proviral gene products (16,44)) cannot be excluded.If this is the case, spot 5 may be an IL-2R-related "fusion protein" ("polyprotein") which escaped proteolytic cleavage, and is glycosylated and inserted into the plasma membrane. Alternatively, p115 may be a functional complex forming a homodimer, or a heteropolymer with unidentified protein(s) functionally related to IL-2R.…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic Processing of Human Interleukin‐2 Receptor (Tac Antigen)

Wano

Uchiyama

Yodoi

et al. 1985

Microbiology and Immunology

View full text Add to dashboard Cite

Biosynthetic processing of the T-cell surface receptor for interleukin-2 was investigated in a cultured human T-cell line MT-1 by means of metabolic and cell surface radiolabeling followed by immunoprecipitation with a monoclonal antireceptor antibody (anti-Tac) and analysis by one-and two-dimensional polyacrylamide gel electrophoresis.The nascent precursor of the receptor (Mr= about 40,000, pI=6.2-6.5) underwent a post-translational modification giving rise to the mature receptor (IL-2R; Mr=60,000-65,000, pI = 4.2-4.7) within 2-4 hr. The post-translational processing of IL-2R caused a 20,000-25,000 increase in apparent molecular weight and a 2.0-2.5 acidic shift in the isoelectric point. The increase in molecular weight was attributable mainly to addition of sugar residues including glucosamine and galactose, and the charge shift to the addition of sialic acids. A carboxylic ionophore monensin completely blocked the maturation of IL-2R at the mid-stage of the processing. Fatty acid attachment appeared to comprise one of the steps of the post-translational modification.Two-dimensional analyses of IL-2R biosynthesis enabled identification of the precursor of IL-2R and its intermediate forms, from which it was partially possible to estimate reactions involved in the maturation of the precursor molecule.

show abstract

Section: Discussionmentioning

confidence: 99%