PSL, an S phase-related p55 nuclear antigen, associates transiently with chromatin

Barque, Jean-Philippe; Lagaye, Sylvie; Bendayan, Moı̈se; Puvion‐Dutilleul, Francine; Danon, F; Larsen, Christian‐Jacques

doi:10.1016/0014-4827(85)90147-8

Cited by 6 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serum Bi. has already been shown to recognize a nuclear antigen with an apparent molecular mass of 55,000 daltons (4)(5)(6). We report here that the sera of these patients immunoprecipitate fr o m HeLa cell extracts 5S RNA and two proteins with apparent molecular masses of 37,000 daltons (p37) and 32,000 daltons (p32).…”

Section: Introductionmentioning

confidence: 70%

See 1 more Smart Citation

Characterization by human antibodies of two HeLa cell proteins which are related toXenopus laevistranscription factor TFIIIA

Lagaye¹,

Barque²,

Maire

et al. 1988

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

The sera of two patients with autoimmune disorders recognize in HeLa cell extracts two proteins with apparent molecular masses of 37,000 (p37) daltons and 32,000 daltons (p32). These proteins are non covalently associated with 5S RNA and sediment as 7-10 S particles in sucrose density gradients. Both proteins are antigenetically related to TFIIIA, a previously described protein of Xenopus laevis, which is known as a 5S RNA transcription factor and occurs in oocytes as a noncovalent complex with 5S RNA. Like TFIIIA, HeLa cell proteins p37 binds in vitro to 5S RNA and to cloned 5S RNA genes. These results suggest that protein p37 fulfils in HeLa cells a function similar to that of TFIIIA in amphibian oocytes, ie control of 5S RNA transcription.

show abstract

Section: Introductionmentioning

confidence: 70%

“…1A, lane 2). One of these bands has already been characterized (4)(5)(6). It corresponds to a 1 2 3 kDa 1 2 kDa The precipitated antigens were recovered from the immune complexes and analysed by polyacrylamide gel electrophoresis followed by autoradiography.…”

Section: Characterization Of Two Hela Cell Proteins Recognized By Hummentioning

confidence: 99%

Characterization by human antibodies of two HeLa cell proteins which are related toXenopus laevistranscription factor TFIIIA

Lagaye¹,

Barque²,

Maire

et al. 1988

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…[32][33][34][35][36][37], or to other cell cycle-specific proteins such as thymidine kinase (38), histones (ref. 39 and references therein), p68 (40), dihydrofolate reductase (41), p55 (42), statin (43), and thymidine synthetase (44). In particular, dividin differs from p53 in its isoelectric point (p53 is more acidic), cell-type specificity (p53 is not present in HeLa cells), and stage of the cell cycle at which it is synthesized [p53 is synthesized in S and part of G1 (36) identified a 55-kDa nonhistone protein in HeLa cells that is preferentially phosphorylated during S phase.…”

Section: Discussionmentioning

confidence: 99%

Proliferation-sensitive nuclear phosphoprotein "dividin" is synthesized almost exclusively during S phase of the cell cycle in human AMA cells.

Celis¹,

Nielsen²

1986

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Studies of the polypeptides synthesized by normal and transformed human cultured cells under a variety of physiological conditions have revealed a basic 54-kDa protein NEPHGE 1Oa, whose rate of synthesis is sensitive to changes in the rate of cell proliferation. This nuclear phosphoprotein, which we have termed "dividin" (present only in populations of cells committed to divide), is synthesized almost exclusively during the S phase of the cell cycle of transformed human amnion cells (AMA). Dividin synthesis is first detected late in G, near the G1/S transition border, reaches a maximum late in S phase, and declines thereafter. As expected for an S phase-specific protein, no detectable synthesis of dividin was observed in growth-arrested normal human cultured cells of epithelial and fibroblast origin. These findings suggest a role for this protein in events leading to DNA replication and cell division.Working with the hypothesis that somatic cell transformation may be due to the abnormal expression of normal genes, we began a detailed study of the overall patterns of gene expression at the level of protein synthesis in normal and transformed human cells to identify proteins that may be involved in the control of cell proliferation (1-4). The ultimate goal of these studies is to elucidate the pathway(s) that controls cell proliferation in normal human cells and, hence, to determine the means by which alterations of the pathway(s) lead to abnormal growth and/or neoplastic transformation and cancer. To date, several growth rate-and transformation-sensitive polypeptides have been identified using high-resolution twodimensional (2D) gel electrophoresis (refs. 2 and 3 and references therein). Of these, the proliferation-sensitive nuclear protein cycin (2)(3)(4)(5)(6), also termed proliferating cell nuclear antigen (PCNA) (7,8), is one of the most striking as the rate of synthesis of this protein increases specifically during S phase (9-11), and its activity may be associated with some specific aspect of DNA replication (12)(13)(14)(15)(16)(17)(18).In this communication we present evidence showing that the proliferation-sensitive 54-kDa human nuclear basic phosphoprotein NEPHGE 10a [ Fig. 1 MATERIALS AND METHODSCells. All the cells used in this study were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% (vol/vol) fetal calf serum and antibiotics (penicillin at 100 units/ml, streptomycin at 50 ,ug/ml).Cell Cycle Studies. Mitotic AMA cells were obtained by mechanical detachment essentially as described by Terasima and Tolmach (22). Two 250-ml flasks containing 1-2 x 106 cells per flask were used. The homogeneity of the collected cells was assessed by phase-contrast microscopy. Cells for [35S]methionine labeling were plated in microtiter wells (96-well plates, Nunc), while those for immunofluorescence were plated on round glass coverslips. Entrance of the cells into S phase was determined by indirect immunofluorescence using PCNA antibodies specific for cyclin (17,23).Quiescent Cultures. MRC-5 hum...

show abstract

Characterization of an Ubiquitous S-Phase Related and Chromatin Associated Antigen with Human Autoantibodies

Barque

Lagaye

Peraudeau

et al. 1985

Protides of the Biological Fluids

View full text Add to dashboard Cite

PSL, an S phase-related p55 nuclear antigen, associates transiently with chromatin

Cited by 6 publications

References 13 publications

Characterization by human antibodies of two HeLa cell proteins which are related toXenopus laevistranscription factor TFIIIA

Characterization by human antibodies of two HeLa cell proteins which are related toXenopus laevistranscription factor TFIIIA

Proliferation-sensitive nuclear phosphoprotein "dividin" is synthesized almost exclusively during S phase of the cell cycle in human AMA cells.

Characterization of an Ubiquitous S-Phase Related and Chromatin Associated Antigen with Human Autoantibodies

Contact Info

Product

Resources

About