The expression of p‐selectin during collection, processing, and storage of platelet concentrates: relationship to loss of in vivo viability

Abstract

These studies show that p-selectin expression on the platelet surface is a predictor of platelet viability, although the extent of shape change and the hypotonic shock response may be more sensitive.

Methods for evaluation of platelet function

“…Surface expression of GPIIb/IIIa, Pselectin and GPIb can be measured by flow cytometry. Increased P-selectin expression during storage has been reported by several authors [15][16][17] whereas GPIb has been shown to decrease during storage [15,17]. It is however unclear whether the level of in vitro platelet activation in stored PCs correlates with in vivo survival and haemostatic function of platelets after transfusion [18].…”

Influence of Different Methods Preparation on Platelet Activation in Stored Platelet Concentrates

“…The studies suggested that the degree of in vitro activation as evidenced by the expression of CD63 and Annexin 142+10 149+12 165+15 223+12▲ 129+9 134+14 142+12 149+10▲ 119+10 121+10 130+10 139+12▲ ▲Significant difference between BC, PC and APC; Values are mean+standard deviation; n=25. [2,4,12,14].…”

“…This study shows that in addition to pH, volume and cell counts; in vitro platelet activation markers were used to monitor platelet [3,13,14]. These data suggest that measurement of CD63, Annexin V and LDH may be a more desirable markers for clinical studies of activated platelets, since it may be less susceptible to artifactual elevation due to minor variations in sample handling and assay [3,12,14]. The results of this study showed that the LDH levels were also increased during storage in platelet concentrates.…”

“…Activation of platelet is followed by an increased CD63 expression; this protein secreted from the granules during storage [2,[12][13][14]. Lactatedehydrogenase (LDH), a marker of disintegration of platelets as a parameter for activation marker of platelet concentrates during storage.…”

Platelet Activation in Stored Platelet Concentrates: Comparision of Two Methods Preparation

“…Platelets processed by the buffy coat technique showed less p-selectin (CD62P) expression than platelets prepared by the PRP method. This study shows that in addition to pH and cell counts; in vitro platelet activation markers were used to monitor platelet quality [11,19]. These data suggest that measurement of CD62P and Annexin V may be a more desirable marker for clinical studies of activated platelets, since it may be less susceptible to artifactual elevation due to minor variations in sample handling and assay procedures [13][14][15].…”