The expression of human alpha and beta defensin in the endometrium and their effect on implantation

Das, Sangeeta; Vince, Gill; Lewis-Jones, Iwan; Bates, Michelle; Gazvani, Rafet

doi:10.1007/s10815-007-9173-2

Cited by 14 publications

(13 citation statements)

References 24 publications

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“…Prior to our work Das et al [34] measured α-defensin 1-3 production in the follicular fluid without finding a correlation with fertilization or IVF outcome. The same authors [35] observed no difference in expression (using immunohistochemistry) of α-defensin 1-3 and hBD-1 in endometrium from patients who did or did not reach pregnancy following subsequent IVF. To note, the endometrial samples were collected at least two cycles before the IVF and therefore may not directly reflect the endometrial status of the treatment cycle accurately.…”

Section: Discussionmentioning

confidence: 87%

Human β-defensin 1 in follicular fluid and semen: impact on fertility

Zupin

Polesello

Martinelli

et al. 2019

J Assist Reprod Genet

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Purpose β-defensins are antimicrobial peptides expressed at mucosal level of male and female genito-urinary tract, where they exert protective functions against infections, possibly preserving human health and fertility. In our study, we investigated the possible involvement of β-defensins in female and male infertility in Italian infertile couples (i) evaluating the presence of human β-defensin 1 (hBD-1) in follicular fluid (FF) and its correlation with in vitro fertilization (IVF) outcomes; (ii) investigating the relationship between hBD-1 levels in semen and IVF outcomes (comprising correlation with sperm parameters); and (iii) exploring the effect of hBD-1 peptide on spermatozoa motility in vitro. Methods A perspective observational analytic pilot study was conducted. hBD-1 concentration was measured with ELISA assay in FF and semen from 50 couples that underwent assisted procreation technique procedures due to infertility status. Moreover, hBD-1 exogenous peptide was administered to 29 normozoospermic semen and their motility was recorded. Results hBD-1 was detected in FF and its levels were significantly higher in women with good fertilization rate (≥ 75%), respect to those with a poor fertilization rate (< 75%). The hBD-1 semen concentrations in oligo-asthenozoospermic subjects were significantly lower than that in normozoospermic men. Instead, hBD-1 level in sperm and FF not correlated with pregnancy rate. Finally, incubation of sperm with exogenous hBD-1 significantly increased progressive motility after 1 h and 24 h. Conclusions Being aware of the relatively small sample size and medium power, our results possibly suggest that hBD-1 could influence oocyte and sperm quality, and could improve, when exogenously added, sperm motility.

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Section: Discussionmentioning

confidence: 87%

Human β-defensin 1 in follicular fluid and semen: impact on fertility

Zupin

Polesello

Martinelli

et al. 2019

J Assist Reprod Genet

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“…Figure 1D). Using the Cell Marker database 19 combined with published literature, [13][14][15]20,21 low PRL expression in decidual stromal cells were found during early pregnancy. 13 In addition to the inconsistent PRL expression in these three subgroups (cluster 2, cluster 6 and cluster 13), GSTA1 and CLDN10 expression differences could be used to distinguish them ( Figure 2K Figure 3C).…”

Section: Pseudotemporal Ordering Analysismentioning

confidence: 99%

Single‐cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period

Huang

Peng

et al. 2020

Cell Proliferation

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“…By immunostaining of the rat endometrium, Alan and Liman (2012) The presence of b-defensin protein in the cytoplasm of epithelial cells and within secretory material is consistent with the intracellular synthesis of b-defensin followed by its intraluminal secretion, most likely as part of the uterine innate immune defence. Similarly, in the human mid-luteal endometrium, the luminal and glandular epithelium as well as glandular secretions were immunopositive for human b-defensin-1 (Das et al 2007). …”

mentioning

confidence: 91%

“…In mucous membranes such as the endometrium, they provide the first line of defence to pathogens and are involved in maintaining the physiological milieu (King et al 2003, Fahey et al 2008, Lyle 2011. Beta-defensins have been detected in the endometrium of human beings (King et al 2003, Jorgenson 2005, Das et al 2007, Fahey et al 2008 and different species including rats (Alan and Liman 2012), mice (Soboll et al 2006) and bovine (Davies et al 2008, Martins et al 2011). …”

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confidence: 99%

Beta-defensin is a component of the endometrial immune defence in the mare

Schöniger¹,

Grafe²,

Schoon³

2013

PHK

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SummaryDefensins are antimicrobial peptides of the innate immune defence. The aim of the present study was to examine if b-defensin is expressed in the equine endometrium. Endometrial tissue samples were available from 29 mares. Performed were a histological examination (n=28), and immunohistochemistry (n =28). Mares aged 3 -20 years. Endometrosis was diagnosed in 20 mares; in the biopsy of 1 mare, endometrial glands could not be evaluated. Endometritis was observed in 12 and angiosclerosis in 21 mares. No endometrial alterations were found in 3 mares. Expression of equine b-defensin-1 mRNA was detected in all 29 mares and confirmed by sequencing in 11 cases. Immunostaining for b-defensin was detected in all 28 mares investigated; however, in 1 mare the surface and in another mare the glandular epithelium could not be evaluated. Immunopositive cell populations were the luminal epithelium (n=27), the glandular epithelium (n=20), stromal cells (n=6) and the vascular tunica media (n=14). The majority of immunopositive cells were located within the luminal epithelium and the duct of the endometrial glands. The immunostainig of the epithelium was predominantly cytoplasmic and less frequently nuclear. Stromal cells showed a nuclear staining. The smooth muscle cells of the vascular tunica media displayed a cytoplasmic labelling in 13 mares and a nuclear staining in 1 mare. This study demonstrates the expression of b-defensins in the healthy and diseased equine endometrium and indicates that b-defensin contributes to the endometrial immune defence in the mare.

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The expression of human alpha and beta defensin in the endometrium and their effect on implantation

Cited by 14 publications

References 24 publications

Human β-defensin 1 in follicular fluid and semen: impact on fertility

Human β-defensin 1 in follicular fluid and semen: impact on fertility

Single‐cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period

Beta-defensin is a component of the endometrial immune defence in the mare

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