Single‐cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period

Huang, Ju; Li, Qi; Peng, Qiaozhen; Xie, Yingming; Wang, Weinan; Pei, Chenlin; Zhao, Yanhua; Liu, Rong; Huang, Lihui; Li, Tieping; Xie, Liling; Zhang, Jiejie; Dai, Lei; Chen, Jingfei; Sun, Jingchi; Zhang, Weishe

doi:10.1111/cpr.12967

Cited by 21 publications

(27 citation statements)

References 49 publications

(139 reference statements)

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“…The processed scRNA-seq data of 29,231 peripartum decidual cells from our previous study were collated (including 17,149 DBD cells and 12,082 DAD cells) (Huang et al, 2021). There were eight main types of peripartum cells, including 10,004 endothelial cells (ECs), 6,422 decidual stromal cells (DSCs), 5,277 extravillous trophoblasts (EVTs), 1,194 T cells (TCs), 3,720 smooth muscle cells (SMCs), 1,312 Dendritic cells (DCs), 1,133 fibroblasts (FBs), and 169 endometrial cells (EECs).…”

Section: Scrna-seq Data Collectionmentioning

confidence: 99%

“…A total of six peripartum decidua samples (three DBD samples and three DAD samples) were obtained from Xiangya Hospital Central South University or Changsha Hospital for Maternal and Child Health Care. Informed consent was obtained from all patients prior to data collection, which are the same samples as our previous study (Huang et al, 2021). The processing of tissue dissociation can be seen in our previous report (Huang et al, 2021).…”

Section: Ethics Statement Informed Consent and Sample Preparationmentioning

confidence: 99%

“…Informed consent was obtained from all patients prior to data collection, which are the same samples as our previous study (Huang et al, 2021). The processing of tissue dissociation can be seen in our previous report (Huang et al, 2021). Then the cell suspension was prepared for the scRNA-seq cDNA library and scBCR/TCR cDNA library preparation and sequencing, respectively.…”

Section: Ethics Statement Informed Consent and Sample Preparationmentioning

confidence: 99%

“…Moreover, scRNA-seq of sorted placental cells from firstand second-trimester human placentae identified several new trophoblast subtypes and human placental trophoblast differentiation during the early stage of pregnancy (Liu et al, 2018). In our previous study, we characterized the single-cell landscape of the peripartum decidua, identified the major cell populations and subpopulations of the decidua, and revealed decidual cell changes during labor (Huang et al, 2021). These cell atlas studies have provided important resources for future explorations of pregnancy and its complications (Rajagopalan and Long, 2018).…”

Section: Introductionmentioning

confidence: 98%

“…In this study, we aimed to visualize the intercellular communication that occurs during the perinatal period and identify key intercellular transduction signaling pathways related to the onset of labor. We collated the scRNA-seq data for term decidua before delivery (DBD) and after delivery (DAD) obtained in our previous study (Huang et al, 2021) and reconstructed the cell-cell crosstalk via the CellCall method (Zhang et al, 2021a). We then investigated the shared and differential intercellular signals between DBD and DAD and explored the intercellular crosstalk between T cells and other decidual cells.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

Huang

Zhang

Zhao

et al. 2021

Front. Cell Dev. Biol.

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Intercellular communication in the decidua plays important roles in relaying information between the maternal and fetal systems in the maintenance of pregnancy and the transition to labor. To date, several studies have explored cell-cell communications in the decidua during different periods of pregnancy, but studies systematically decoding the intercellular communication network, its internal cascades, and their involvement in labor are still lacking. In this study, we reconstructed a decidual cell-cell communication network based on scRNA-seq of peripartum decidua via the CellCall method. The results showed that endometrial cells (EECs) and extravillous trophoblasts relayed most of the common intercellular signals in the decidua both before delivery (DBD) and after delivery (DAD). Endothelial cells and EECs controlled many WNT-signaling-related intercellular communication factors that differed between DBD and DAD, some of which could be candidate biomarkers for the diagnosis of labor. Analysis of intercellular communications related to T cells identified abundant maternal-fetal immune-tolerance-related communication, such as TNFSF14-TNFRSF14/LTBR and FASLG-FAS signalings. We further explored the characteristics of the B cell receptor (BCR) and T cell receptor (TCR) repertoires by single-cell BCR/TCR sequencing. The results showed no significant differences in clonal expansion of B/T cells between DAD and DBD, indicating there was no significant change to adaptive immunity at the maternal-fetal interface during delivery. In summary, the findings provide a comprehensive view of the intercellular communication landscape in the peripartum decidua and identified some key intercellular communications involved in labor and maternal-fetal immune tolerance. We believe that our study provides valuable clues for understanding the mechanisms of pregnancy and provides possible diagnostic strategies for the onset of labor.

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Section: Scrna-seq Data Collectionmentioning

confidence: 99%

Section: Ethics Statement Informed Consent and Sample Preparationmentioning

confidence: 99%

Section: Ethics Statement Informed Consent and Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

Huang

Zhang

Zhao

et al. 2021

Front. Cell Dev. Biol.

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MAX deficiency impairs human endometrial decidualization through down-regulating OSR2 in women with recurrent spontaneous abortion

Cao

et al. 2022

Cell Tissue Res

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Human uterine stromal cell undergoes decidualization for pregnancy establishment and maintenance, which involved extensive proliferation and differentiation. Increasing studies have suggested that recurrent spontaneous abortion (RSA) may result from defective endometrial stromal decidualization. However, the critical molecular mechanisms underlying impaired decidualization during RSA are still elusive. By using our recently published single-cell RNA sequencing (scRNA-seq) atlas, we found that MYC-associated factor X (MAX) was significantly downregulated in the stromal cells derived from decidual tissues of women with RSA, followed by verification with immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). MAX knockdown significantly impairs human endometrial stromal cells (HESCs) proliferation as determined by MTS assay and Ki67 immunostaining, and decidualization determined by F-actin, and decidualization markers. RNA-seq together with chromatin immunoprecipitation sequencing (ChIP-seq) and cleavage under targets and release using nuclease sequencing (CUT&RUN-seq) analysis were applied to explore the molecular mechanisms of MAX in regulation of decidualization, followed by dual-luciferase reporter assay to verify that MAX targets to (odd-skipped related transcription factor 2) OSR2 directly. Reduced expression of OSR2 was also confirmed in decidual tissues in women with RSA by IHC and qRT-PCR. OSR2 knockdown also significantly impairs HESCs decidualization. OSR2-overexpression could at least partly rescue the downregulated insulin-like growth factor binding protein 1 (IGFBP1) expression level in response to MAX knockdown. Collectively, MAX deficiency observed in RSA stromal cells not only attenuates HESCs proliferation but also impairs HESCs decidualization by downregulating OSR2 expression at transcriptional level directly.

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A single-cell atlas of murine reproductive tissues during preterm labor

et al. 2023

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Single‐cell RNA sequencing reveals heterogeneity and differential expression of decidual tissues during the peripartum period

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 21 publications

References 49 publications

Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

Deciphering the Intercellular Communication Network of Peripartum Decidua that Orchestrates Delivery

MAX deficiency impairs human endometrial decidualization through down-regulating OSR2 in women with recurrent spontaneous abortion

A single-cell atlas of murine reproductive tissues during preterm labor

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