The Expression of Heat Shock Protein and Cognate Genes During Plant Development

Winter, Jill; Sinibaldi, Ralph M.

doi:10.1007/978-3-540-46712-0_7

Cited by 20 publications

(15 citation statements)

References 119 publications

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“…Indeed, numerous members of at least three major HSP families (70 kD, 60 kD, and low-molecularmass HSP) were already expressed at this temperature. Although such expression at control temperature might also be partly induced or enhanced by the isolation procedure and (or) culture of the cells in artificial conditions, these results are in agreement with reports describing the expression of HSPs at normal growth temperature in several systems, including plant systems (reviewed by Winter and Sinibaldi, 1991). For instance, the constitutive forms of HSP70 and HSP60 appear to be involved in normal cellular processes such as protein folding and intracellular targeting.…”

Section: Expression Of Microspore Hsps At Non-heat-shock Temperaturesupporting

confidence: 91%

“…In addition to their implication in general cellular processes, a growing amount of evidence suggests more specific roles for certain HSPs during cell proliferation processes and development in a variety of eukaryotic organisms (Hightower and Nover, 1991;Winter and Sinibaldi, 1991;Lane et al, 1993). For instance, HSP70 expression has been reported during meiosis of mammalian spermatogenesis (Wolgemuth and Gruppi, 1991).…”

Section: Expression Of Microspore Hsps At Non-heat-shock Temperaturementioning

confidence: 99%

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Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

1996

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l h e expression of heat-shock proteins (HSPs) in isolated maize (Zea mays 1.) microspores has been investigated using highresolution two-dimensional electrophoresis coupled to immunodetection and fluorography of in vivo synthesized proteins. To this end, homogeneous and viable populations of microspores have been purified in sufficient amounts for molecular analysis from plants grown in controlled conditions. Appropriate conditions for thermal stress application have been defined. The analysis revealed that isolated microspores from maize display a classical heat-shock response characterized by the repression of the normal protein synthesis and the expression of a set of HSPs. A high complexity of the response was demonstrated, with numerous different HSPs being resolved in each known major H S P molecular weight class. However, the extent of this heat-shock response is limited in that some of these HSPs do not accumulate at high levels following temperature elevation. Comparative analysis of the heat-shock responses of microspores isolated from five genotypes demonstrated high levels of genetic variability. Furthermore, many HSPs were detected in microspores at control temperature, indicating a possible involvement of these proteins in pollen development at stages close to first pollen mitosis.

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Section: Expression Of Microspore Hsps At Non-heat-shock Temperaturesupporting

confidence: 91%

Section: Expression Of Microspore Hsps At Non-heat-shock Temperaturementioning

confidence: 99%

Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

1996

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“…Hsps belong to a large family that has been classified into several groups, including Hsp60, Hsp70, Hsp90, Hsp100, and the small Hsps (for review, see Vierling, 1991;Winter and Sinibaldi, 1991;Miernyk, 1999). It is well known that Hsps are significantly induced when cells or organisms are exposed to supraoptimal temperatures or other stresses.…”

Section: Genes That May Be Functionally Involved In Pg and Ptgmentioning

confidence: 99%

Transcriptome Analyses Show Changes in Gene Expression to Accompany Pollen Germination and Tube Growth in Arabidopsis

et al. 2008

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Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step toward a detailed understanding of this important process, the objective of this study was to comprehensively analyze the transcriptome changes during pollen germination and pollen tube growth. Using Affymetrix Arabidopsis (Arabidopsis thaliana) ATH1 Genome Arrays, this study is, to our knowledge, the first to show the changes in the transcriptome from desiccated mature pollen grains to hydrated pollen grains and then to pollen tubes of Arabidopsis. The number of expressed genes, either for total expressed genes or for specifically expressed genes, increased significantly from desiccated mature pollen to hydrated pollen and again to growing pollen tubes, which is consistent with the finding that pollen germination and tube growth were significantly inhibited in vitro by a transcriptional inhibitor. The results of Gene Ontology analyses showed that expression of genes related to cell rescue, transcription, signal transduction, and cellular transport was significantly changed, especially for up-regulation, during pollen germination and tube growth. In particular, genes of the calmodulin/calmodulin-like protein, cation/hydrogen exchanger, and heat shock protein families showed the most significant changes during pollen germination and tube growth. These results demonstrate that the overall transcription of genes, both in the number of expressed genes and in the levels of transcription, was increased. Furthermore, the appearance of many novel transcripts during pollen germination as well as tube growth indicates that these newly expressed genes may function in this complex process.

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“…Similar observations in plants are still scarce [reviews in 4,5], although there are examples showing expression of specific HSP genes during pollen development [6,7], fungal sporulation [8], and in zygotic [9][10][11] as well as somatic embryogenesis [12,13]. Some of these examples include LMW HSP genes [6,7,10,12,13], but in most cases only mRNA accumulation or in vitro translation data have been reported.…”

Section: Introductionmentioning

confidence: 96%

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

1994

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We isolated and sequenced Ha hsp17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hspl7.9, and the previously reported Ha hspl7.6, as cDNAs encoding proteins (HSP17.6 and H S P 17.9) which belong to different families of cytoplasmic LMW H S Ps. Using specific antibodies we observed differential expression of both proteins during zygotic embryogenesis under controlled environment, and a remarkable persistence of these LMW HSPs during germination. Immuno-blot analysis of HSP17.9 proteins in two-dimensional gels revealed that the polypeptides expressed in embryos were indistinguishable from LMW HSPs expressed in vegetative tissues in response to water deficit; but they appeared different from homologeous proteins expressed in response to thermal-stress. Tissue-print immunolocalization experiments showed that HSP17.9 and HSP17.6 were homogeneously distributed in every tissue of desiccation-tolerant dry seeds and young seedlings under non-stress conditions. These results demonstrate developmental regulation of specific, cytoplasmic, plant LMW HSPs, suggesting also their involvement in water-stress tolerance.

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The Expression of Heat Shock Protein and Cognate Genes During Plant Development

Cited by 20 publications

References 119 publications

Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

Transcriptome Analyses Show Changes in Gene Expression to Accompany Pollen Germination and Tube Growth in Arabidopsis

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

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