Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

Magnard, Jean‐Louis; Vergne, Philippe; Dumas, Christian

doi:10.1104/pp.111.4.1085

Cited by 31 publications

(21 citation statements)

References 36 publications

(60 reference statements)

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“…5, N and O). This observation is consistent with previous findings in maize (Zea mays; Marrs et al, 1993;Magnard et al, 1996), indicating the significance of chaperones and in particular a prominent role of the AtHsp90-1 in Arabidopsis pollen development. After heat shock, GUS staining was prominent in all parts of the developing flower (Fig.…”

Section: Discussionsupporting

confidence: 93%

Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1Gene

et al. 2002

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The promoter region of the Arabidopsis AtHsp90-1 gene is congested with heat shock elements and stress response elements, as well as with other potential transcriptional binding sites (activating protein 1, CCAAT/enhancer-binding protein element, and metal regulatory element). To determine how the expression of this bona fide AtHsp90-1 gene is regulated, a comprehensive quantitative and qualitative promoter deletion analysis was conducted under various environmental conditions and during development. The promoter induces gene expression at high levels after heat shock and arsenite treatment. However, our results show that the two stress responses may involve common but not necessarily the same regulatory elements. Whereas for heat induction, heat shock elements and stress response elements act cooperatively to promote high levels of gene expression, arsenite induction seems to require the involvement of activating protein 1 regulatory sequences. In stressed transgenic plants harboring the full-length promoter, ␤-glucuronidase activity was prominent in all tissues. Nevertheless, progressive deletion of the promoter decreases the level of expression under heat shock and restricts it predominantly in the two meristems of the plant. In contrast, under arsenite induction, proximal sequences induce AtHsp90-1 gene expression only in the shoot meristem. Distally located elements negatively regulate AtHsp90-1 gene expression under unstressed conditions, whereas flower-specific regulated expression in mature pollen grains suggests the prominent role of the AtHsp90-1 in pollen development. The results show that the regulation of developmental expression, suppression, or stress induction is mainly due to combinatorial contribution of the cis elements in the promoter region of the AtHsp90-1 gene.During their lifetime, plant species can be subjected to various stressful environments to which they respond and adapt by means of physiological, developmental, and biochemical changes. One of the most thoroughly characterized is the induction of heat shock proteins (HSPs) when cells or organisms are exposed to supraoptimal temperatures and other types of stresses (for review, see Lindquist and Craig, 1988;Vierling, 1991;Miernyk, 1999). The heat shock response is a universal (Schlessinger et al., 1982;Morimoto and Santoro, 1998) and evolutionarily conserved phenomenon (Schlessinger et al., 1982). However, it is now recognized that the same or closely related proteins are frequently essential components of cells under normal physiological conditions (Boston et al., 1996).Accumulating evidence reveals that all of the major HSPs serve as molecular chaperones (Georgopoulos and Welch, 1993;Bukau and Horwich, 1998;Pratt et al., 2001). Although the structure and the mechanism of some chaperones such as HSP70, HSP60, and sHSPs have been investigated extensively (Waters et al., 1996;Bukau and Horwich, 1998), the function of HSP90s as molecular chaperones is still controversial. The HSP90s are among the most highly conserved proteins known, w...

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Section: Discussionsupporting

confidence: 93%

Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1Gene

et al. 2002

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“…It is well known that different HSPs are abundantly expressed during pollen development in maize (27,35,36). Indeed, as seen in Fig.…”

Section: Constitutive Expression Of Hsp Genes In Maize Mitochondrialmentioning

confidence: 90%

Mitochondrial Respiratory Deficiencies Signal Up-regulation of Genes for Heat Shock Proteins

Kuzmin

Карпова

Elthon

et al. 2004

Journal of Biological Chemistry

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The consequences of mitochondrial dysfunction are not limited to the development of oxidative stress or initiation of apoptosis but can result in the establishment of stress tolerance. Using maize mitochondrial mutants, we show that permanent mitochondrial deficiencies trigger novel Ca 2؉ -independent signaling pathways, leading to constitutive expression of genes for molecular chaperones, heat shock proteins (HSPs) of different classes. The signaling to activate hsp genes appears to originate from a reduced mitochondrial transmembrane potential. Upon depolarization of mitochondrial membranes in transient assays, gene induction for mitochondrial HSPs occurred more rapidly than that for cytosolic HSPs. We also demonstrate that in the nematode Caenorhabditis elegans transcription of hsp genes can be induced by RNA interference of nuclear respiratory genes. In both organisms, activation of hsp genes in response to mitochondrial impairment is distinct from their responses to heat shock and is not associated with oxidative stress. Thus, mitochondria-to-nucleus signaling to express a hsp gene network is apparently a widespread retrograde mechanism to facilitate cell defense and survival.

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“…It is likely that some of these new proteins (40, 29 and 26 kDa) might have helped in H. antidysenterica to encounter stressful environments. In the earlier work, several proteins such as (LEA, HSP, RAB) isolated from higher plants have already been shown to play a very significant role in abiotic stresses (Singla and Grover 1994, Magnard et al 1996, Hvoslef-Eide and Croke 1997, Sundar et al 2003. In more recent study, boiling stable protein of 66 kDa in Sesbania sesban var.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 96%

Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica

Agrawal¹,

Sharma²

2006

Biologia plant.

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The nodal explants of in vitro shoots of Holarrhena antidysenterica L. were cultured on Murashige and Skoog's (MS) medium augmented with 15 µM N 6 -benzyladenine (BA) alone (control) or supplemented with different concentrations (1, 5, 10 and 20 mg dm -3 ) of CdCl 2 , CuSO 4, Pb(NO 3 ) 2 and ZnSO 4 . The maximum morphogenic response in terms of average shoot number (4.95 ± 0.17) was seen in control. ZnSO 4 proved to be less inhibitory in comparison to CuSO 4 , Pb(NO 3 ) 2 and CdCl 2 . None of the explants cultured on CdCl 2 containing medium induced multiple shoots. Maximum protein content [3.80 ± 0.04 mg g -1 (f.m.)] was observed in control and slightly less [3.50 ± 0.02 mg g -1 (f.m.)] in tissues exposed to 1 mg dm -3 of CuSO 4 and minimum [1.00 ± 0.02 mg g -1 (f.m.)] in Zn treated (20 mg dm -3 ) explants. SDS-PAGE analysis of the treated tissues revealed that two new polypeptides (29 and 20 kDa) in response to Cu and Zn treatment, respectively, have been synthesized.

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Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

Cited by 31 publications

References 36 publications

Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1Gene

Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1Gene

Mitochondrial Respiratory Deficiencies Signal Up-regulation of Genes for Heat Shock Proteins

Phytotoxic effects of Cu, Zn, Cd and Pb on in vitro regeneration and concomitant protein changes in Holarrhena antidysenterica

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